Podcast
Questions and Answers
Allosteric enzymes display a _______ curve in contrast to the hyperbolic curve displayed by Michaelis-Menten Enzymes.
Allosteric enzymes display a _______ curve in contrast to the hyperbolic curve displayed by Michaelis-Menten Enzymes.
sigmoidal
Most allosteric enzymes contain multiple sub-units which can affect each other when the substrate binds to the _______.
Most allosteric enzymes contain multiple sub-units which can affect each other when the substrate binds to the _______.
enzyme
Inhibition can affect either K0.5, which is the substrate concentration for _______-saturation, Vmax or both.
Inhibition can affect either K0.5, which is the substrate concentration for _______-saturation, Vmax or both.
half
Allosteric enzymes have two states: a low-affinity state dubbed the “T” state and the high-affinity “R” state. Inhibitors work by preferentially binding to the ___ state of an allosteric enzyme.
Allosteric enzymes have two states: a low-affinity state dubbed the “T” state and the high-affinity “R” state. Inhibitors work by preferentially binding to the ___ state of an allosteric enzyme.
Signup and view all the answers
Phosphorylation provides another mechanism by which enzymes can be _______.
Phosphorylation provides another mechanism by which enzymes can be _______.
Signup and view all the answers
Enzymes can also be secreted in an inactive state, known as _______.
Enzymes can also be secreted in an inactive state, known as _______.
Signup and view all the answers
Enzymes catalyse a reaction by reducing the activation energy needed for the reaction to occur. However, enzymes need to be tightly regulated to ensure that levels of the product do not rise to ______ levels.
Enzymes catalyse a reaction by reducing the activation energy needed for the reaction to occur. However, enzymes need to be tightly regulated to ensure that levels of the product do not rise to ______ levels.
Signup and view all the answers
Types of Inhibition: Reversible and irreversible inhibitors are chemicals which bind to an enzyme to suppress its activity. One method to accomplish this is to almost permanently bind to an enzyme. These types of inhibitors are called ______.
Types of Inhibition: Reversible and irreversible inhibitors are chemicals which bind to an enzyme to suppress its activity. One method to accomplish this is to almost permanently bind to an enzyme. These types of inhibitors are called ______.
Signup and view all the answers
Reversible inhibitors either bind to an active site (competitive inhibitors), or to another site on the enzyme (non-competitive inhibitors). Competitive inhibitors compete with the substrate at the active site and therefore increase ______ (the Michaelis-Menten constant).
Reversible inhibitors either bind to an active site (competitive inhibitors), or to another site on the enzyme (non-competitive inhibitors). Competitive inhibitors compete with the substrate at the active site and therefore increase ______ (the Michaelis-Menten constant).
Signup and view all the answers
However, Vmax is unchanged because, with enough substrate concentration, the reaction can still ______.
However, Vmax is unchanged because, with enough substrate concentration, the reaction can still ______.
Signup and view all the answers
The graph plot of enzyme activity against substrate concentration would be shifted to the right due to the increase of the Km, whilst the Lineweaver-Burke plot would be ______ when compared with no inhibitor.
The graph plot of enzyme activity against substrate concentration would be shifted to the right due to the increase of the Km, whilst the Lineweaver-Burke plot would be ______ when compared with no inhibitor.
Signup and view all the answers
Non-competitive inhibitors bind to another location on the enzyme and as such decrease ______.
Non-competitive inhibitors bind to another location on the enzyme and as such decrease ______.
Signup and view all the answers
