Enzymatic Reaction Kinetics and Regulation Quiz
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Questions and Answers

What type of molecules are enzymes?

  • Protein molecules (correct)
  • Lipid molecules
  • RNA molecules
  • Carbohydrate molecules

What is the function of enzymes in cellular activity?

  • Maintaining temperature
  • Speeding up chemical reactions (correct)
  • Regulation of pH
  • Stabilizing hormones

What is the main characteristic of enzymes in relation to their substrates?

  • They have no effect on substrates
  • They have high specificity (correct)
  • They have low specificity
  • They form covalent bonds with substrates

Under what conditions do enzymes function?

<p>Aqueous solutions under mild conditions of temperature and pH (B)</p> Signup and view all the answers

What is the main role of an enzyme in a chemical reaction?

<p>To increase the speed of the reaction without suffering changes (A)</p> Signup and view all the answers

What is the specific molecular environment provided by enzymes for chemical reactions?

<p>An energetically favorable environment (D)</p> Signup and view all the answers

What is the main factor that the Gibbs Free Energy (∆Go) depends on?

<p>The final state (A)</p> Signup and view all the answers

In which type of reaction does the free-energy change (∆G) have a negative value?

<p>Exergonic reaction (B)</p> Signup and view all the answers

In which type of reaction do the products contain more free energy than the reactants?

<p>Endergonic reaction (A)</p> Signup and view all the answers

What happens to the reaction when ∆G is large and negative?

<p>The reaction tends to go in the forward direction (C)</p> Signup and view all the answers

In competitive inhibition, how does the intercept on the plot of 1/V versus 1/[S] change in the presence of inhibitor?

<p>It remains the same (C)</p> Signup and view all the answers

What is the energy that cells can and must use, described by the Gibbs free-energy function G?

<p>Free energy (C)</p> Signup and view all the answers

What is the main effect of noncompetitive inhibition on Vmax and the intercept on the vertical axis?

<p>Vmax decreases, intercept increases (D)</p> Signup and view all the answers

What is the main function of the Michaelis-Menten model in enzymatic reactions?

<p>To measure the rate of enzyme activity (B)</p> Signup and view all the answers

Which type of inhibition cannot be overcome by increasing the concentration of substrate?

<p>Reversible noncompetitive inhibition (D)</p> Signup and view all the answers

What does Km represent in the Michaelis-Menten equation?

<p>The enzyme's affinity for the substrate (C)</p> Signup and view all the answers

What is the characteristic feature of irreversible inhibitors?

<p>They chemically modify the enzyme and tightly bind to it (B)</p> Signup and view all the answers

What does Vmax indicate in enzymatic reactions?

<p>The concentration of active sites in fully saturated enzymes (B)</p> Signup and view all the answers

What is the role of modulators in allosteric enzymes?

<p>They regulate enzyme activity by binding to a different site than the active site (D)</p> Signup and view all the answers

What does a large and negative ∆G value indicate for a chemical reaction under standard conditions?

<p>The reaction proceeds spontaneously (A)</p> Signup and view all the answers

What is the effect of competitive inhibitors on Km and Vmax?

<p>$K_m$ increases, $V_{max}$ decreases (B)</p> Signup and view all the answers

What does a large and positive ∆G value indicate for a chemical reaction?

<p>The products contain more free energy than the reactants (A)</p> Signup and view all the answers

Which type of inhibition involves simultaneous binding of inhibitor and substrate to the enzyme?

<p>$Noncompetitive$ inhibition (C)</p> Signup and view all the answers

What is unique for each enzyme and inversely proportional to its affinity for the substrate?

<p>Km value (B)</p> Signup and view all the answers

What is a characteristic feature of reversible competitive inhibitors?

<p>$K_m$ increases, $V_{max}$ does not change (D)</p> Signup and view all the answers

In what conditions does Vmax occur during an enzymatically catalyzed reaction?

<p>When all active sites are occupied by substrate molecules (A)</p> Signup and view all the answers

What distinguishes allosteric enzymes from Michaelis-Menten enzymes?

<p>$Allosteric$ enzymes do not follow Michaelis-Menten kinetics (B)</p> Signup and view all the answers

In noncompetitive inhibition, what happens to Km and Vmax?

<p>Km decreases, Vmax increases (A)</p> Signup and view all the answers

Which type of inhibitors competitively bind to allosteric sites instead of active sites?

<p>Noncompetitive inhibitors (C)</p> Signup and view all the answers

Which type of NSAID inhibits only COX-2?

<p>Celecoxib (A)</p> Signup and view all the answers

What is the function of COX-1 according to the widely held belief?

<p>Produces homeostatic PGs (B)</p> Signup and view all the answers

Which type of pain directly results from a lesion in nerves and the somatosensory system?

<p>Neuropathic pain (C)</p> Signup and view all the answers

What is the primary function of NSAIDs?

<p>Analgesic and anti-inflammatory (B)</p> Signup and view all the answers

Which NSAID is classified as a non-selective inhibitor of COX?

<p>Diclofenac sodium (D)</p> Signup and view all the answers

What is the main effect of selective NSAIDs?

<p>Inhibiting only COX-2 (A)</p> Signup and view all the answers

Which type of NSAID is known for its antipyretic action?

<p>Ibuprofen (C)</p> Signup and view all the answers

What is the primary source of belief regarding the production of PGs by COX-1 and COX-2?

<p>&quot;Widely held&quot; belief (C)</p> Signup and view all the answers

Which type of pain affects structures in the mouth, such as gingival and subgingival tissue?

<p>&quot;Somatic&quot; pain (A)</p> Signup and view all the answers

"Arthrotec" is a combination of which two compounds known for inhibiting COX?

<p>&quot;Diclofenac sodium&quot; and &quot;Misoprostol&quot; (B)</p> Signup and view all the answers

Which type of amino acids in the active site directly participate in the chemical reaction?

<p>Catalytic amino acids (B)</p> Signup and view all the answers

What type of molecules are holoenzymes?

<p>Non-protein portion (C)</p> Signup and view all the answers

What is the region in the enzyme molecule where the enzymatic catalysis takes place?

<p>Active site (A)</p> Signup and view all the answers

What do coenzymes provide during the enzymatic process?

<p>Energy and electrons (A)</p> Signup and view all the answers

What is the essence of catalysis in enzymatic reactions?

<p>Decrease in activation energy (D)</p> Signup and view all the answers

What is the energy derived from enzyme-substrate interactions called?

<p>Binding energy (A)</p> Signup and view all the answers

What determines the speed of a chemical reaction according to the text?

<p>$\Delta G^\ddagger$ (Activation energy) (A)</p> Signup and view all the answers

What is the transition state in a chemical reaction?

<p>$S^\ddagger$ (D)</p> Signup and view all the answers

What does the ground state represent in a reaction coordination diagram?

<p>$S \rightarrow P$ (A)</p> Signup and view all the answers

What is the function of coenzymes in enzymatic reactions?

<p>To facilitate the catalysis by being non-covalently bound to the enzyme (A)</p> Signup and view all the answers

What is the main function of vitamin C (ascorbic acid) in enzymatic reactions?

<p>Avoids oxidation of other vitamins (B)</p> Signup and view all the answers

What do transferases do in enzymatic reactions?

<p>Transfer a chemical group to another molecule (A)</p> Signup and view all the answers

What is the characteristic of hydrolases in enzymatic reactions?

<p>Special type of transferases that transfer a OH from water to another substrate (B)</p> Signup and view all the answers

What is the role of cyclooxigenase (COX) in enzymatic reactions?

<p>Catalyze the first step in the synthesis of prostaglandins (PGs) from arachidonic acid (A)</p> Signup and view all the answers

What is the primary function of COX-1 according to its distribution and expression?

<p>Widely distributed and constitutively expressed in most tissues (B)</p> Signup and view all the answers

What is the effect of pH on enzymes in enzymatic reactions?

<p>Acts as a denaturing agent of proteins and thus to enzymes (B)</p> Signup and view all the answers

What is the role of ligases in enzymatic reactions?

<p>Catalyze the formation of carbon-carbon bonds, requiring ATP energy (B)</p> Signup and view all the answers

What do isomerases catalyze in enzymatic reactions?

<p>(Isomerases) Catalyze the movement of a group or double bond within a molecule producing a new isomer (B)</p> Signup and view all the answers

What do oxidoreductases catalyze in enzymatic reactions?

<p>Catalyze addition or removal of groups to double bonds; formation of double bonds by removal of groups (D)</p> Signup and view all the answers

What is the main function of metal ions as cofactors in enzymatic reactions?

<p>To participate in diverse functions such as oxidation-reduction and promoting hydrolysis (B)</p> Signup and view all the answers

What type of reaction do hydrolases catalyze in enzymatic reactions?

<p>Hydrolysis reactions (A)</p> Signup and view all the answers

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