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Enzymatic Differences in DNA Replication

10 Questions

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Enzymatic Differences in DNA Replication

Created by

@CommendableSard7063

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Questions and Answers

What primary factor distinguishes the sensitivity of RNA polymerase during DNA replication?

Resistance to specific DNA inhibitors

Sensitivity to temperature changes

Dependency on magnesium ions

Inhibition by rifampicin (correct)

How many primers does the leading strand require for synthesis?

One primer synthesized at the ori site (correct)

Two primers for continuous synthesis

Multiple primers at different sites

No primers are needed at all

What characterizes the synthesis of the lagging strand during DNA replication?

It does not require any primers

It initiates with Okazaki fragments (correct)

It uses a single primer for all fragments

Synthesis occurs in a continuous manner

What is the nature of the primer used on the lagging strand?

It is synthesized multiple times for each Okazaki fragment Signup and view all the answers

Which of the following statements about DNA replication in E. coli is true?

The lagging strand consists of discrete Okazaki fragments initiated by primers. Signup and view all the answers

What is the characteristic of the leading strand during DNA replication?

It only requires priming once. Signup and view all the answers

Which of the following statements accurately describes the lagging strand?

It consists of Okazaki fragments. Signup and view all the answers

How does the initiation of leading-strand synthesis get affected by rifampicin in E. coli?

It blocks RNA polymerase, halting the synthesis. Signup and view all the answers

What is the role of primers in synthesizing the lagging strand?

Each Okazaki fragment must begin on a primer. Signup and view all the answers

What distinguishes the leading strand from the lagging strand during DNA replication?

The leading strand is continuous and requires only one primer. Signup and view all the answers

Study Notes

Enzymatic Differences in DNA Replication

Antibiotics can be used to differentiate between enzymes.
Rifampicin inhibits RNA polymerase, but not primase.
Leading strand initiation is sensitive to rifampicin, suggesting RNA polymerase is involved.
Leading and lagging strand primer synthesis differs.
The leading strand needs only one primer at the ori site.
The lagging strand is synthesized in short fragments (Okazaki fragments) that each require a primer.

Enzymatic Differences in DNA Replication

Rifampicin inhibits RNA polymerase, but not primase.
Leading strand synthesis is sensitive to rifampicin because its initiation requires RNA polymerase.
Leading strand only needs to be primed once at the ori site for continuous synthesis.
Lagging strand synthesis occurs in fragments known as Okazaki fragments.
Each Okazaki fragment requires a separate primer.

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Description

This quiz explores the enzymatic mechanisms involved in DNA replication, highlighting the differences between leading and lagging strand synthesis. It examines the role of various antibiotics, such as rifampicin, in distinguishing between different polymerases and their impact on primer synthesis. Test your understanding of these critical biological processes.