Environmental Regulations Quiz

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Questions and Answers

What is the primary responsibility of the Environmental Protection Agency (EPA)?

  • Regulating the quality of drinking water
  • Managing financial aspects of water services
  • Protecting and improving the environment (correct)
  • Ensuring water is provided to every household

Which organization is responsible for public health in relation to drinking water standards?

  • Uisce Éireann
  • Health Service Executive (HSE) (correct)
  • Environmental Protection Agency (EPA)
  • Commission for Regulation of Utilities (CRU)

What do the Drinking Water Regulations aim to ensure?

  • Water suppliers do not need to comply with standards
  • Drinking water quality meets international standards (correct)
  • Public water is provided at no charge
  • Public water supplies are only for agricultural use

Who oversees the drinking water quality for private water suppliers?

<p>Local Authorities (A)</p> Signup and view all the answers

What is one of the main areas of focus for the EPA?

<p>Providing high-quality environmental data and information (B)</p> Signup and view all the answers

Which organization is primarily responsible for enforcing drinking water regulations for public water supplies?

<p>Uisce Éireann (D)</p> Signup and view all the answers

What role does the Commission for Regulation of Utilities (CRU) play?

<p>Ensuring economical and efficient operation of Uisce Éireann (C)</p> Signup and view all the answers

What is a consequence of failing to meet drinking water standards?

<p>Increased public health risks (D)</p> Signup and view all the answers

What is the main purpose of the European Union (Drinking Water) Regulations 2023?

<p>To ensure water is wholesome and clean for human consumption (D)</p> Signup and view all the answers

Which parameter must drinking water be free from according to the regulations?

<p>Escherichia coli (E.coli) (D)</p> Signup and view all the answers

What is the parametric value for acrylamide in drinking water as per the new regulations?

<p>0.10 μg/l (D)</p> Signup and view all the answers

What is indicated by the Chemical Oxygen Demand (COD) test?

<p>The amount of oxygen required to chemically oxidize organic material (C)</p> Signup and view all the answers

What is the typical concentration range for Biochemical Oxygen Demand (BOD) in domestic wastewater?

<p>150-500 mg/L (A)</p> Signup and view all the answers

Which process is NOT part of primary wastewater treatment?

<p>Aeration (B)</p> Signup and view all the answers

What is the primary function of sedimentation in wastewater treatment?

<p>To separate solid and particulate materials from wastewater (B)</p> Signup and view all the answers

Which of the following is a characteristic of the secondary treatment process?

<p>It relies on aerobic and anaerobic processes (A)</p> Signup and view all the answers

Which parameter indicates the organic strength of wastewater?

<p>Biochemical Oxygen Demand (BOD) (C)</p> Signup and view all the answers

How does flocculation aid in wastewater treatment?

<p>By binding suspended particles together (A)</p> Signup and view all the answers

Which statement about dissolved oxygen (DO) measurements is correct?

<p>DO should be measured at the same time each day (B)</p> Signup and view all the answers

What role do grit chambers play in wastewater treatment?

<p>They slow down wastewater flow to allow solids to settle (B)</p> Signup and view all the answers

What is a potential danger of untreated wastewater?

<p>It can support harmful microbial growth (D)</p> Signup and view all the answers

Which of the following is NOT a microbial benefit in wastewater treatment?

<p>Production of drinking water (B)</p> Signup and view all the answers

What is a major consequence of excessive phosphorus in water bodies?

<p>Corrosion of water pipes (C)</p> Signup and view all the answers

Which method is most commonly used to remove phosphorus from water?

<p>Chemical precipitation (D)</p> Signup and view all the answers

What role do Polyphosphate-accumulating organisms (PAOs) play in water treatment?

<p>They help in removing excess phosphorus (A)</p> Signup and view all the answers

Which step follows aeration in the drinking water purification process?

<p>Settling (D)</p> Signup and view all the answers

What is the purpose of coagulation in drinking water purification?

<p>To form heavier particles from light solids (B)</p> Signup and view all the answers

What does the filtration process primarily remove from water?

<p>Microorganisms and impurities (C)</p> Signup and view all the answers

Which disinfectant is commonly used in the final purification of drinking water?

<p>Chlorine gas (B)</p> Signup and view all the answers

Which of the following is a disadvantage of phosphorus in water bodies?

<p>Promotes excessive algae growth (A)</p> Signup and view all the answers

What happens to pathogens in water during the storage phase?

<p>Their numbers decrease significantly (A)</p> Signup and view all the answers

What occurs during flocculation in water treatment?

<p>Coagulated particles settle out (B)</p> Signup and view all the answers

Which of these processes involves using calcium hydroxide or lime?

<p>Chemical precipitation (B)</p> Signup and view all the answers

What is the purpose of sedimentation in drinking water treatment?

<p>To allow solids to settle out (D)</p> Signup and view all the answers

Why is it necessary to reduce turbidity in drinking water?

<p>It makes the water safer by removing harmful particles (D)</p> Signup and view all the answers

What is one of the effects of algal blooms caused by excessive phosphorus?

<p>Skin irritation upon contact (A)</p> Signup and view all the answers

What is the primary function of a trickling filter in wastewater treatment?

<p>To oxidize organic load in wastewater (B)</p> Signup and view all the answers

Which component is essential for the formation of flocs in activated sludge processes?

<p>Zoogloea ranigera (C)</p> Signup and view all the answers

What percentage of secondary sludge is typically recirculated back into the aeration tank?

<p>30% (D)</p> Signup and view all the answers

Which biological treatment stage involves the breakdown of large organic matter into smaller monomers?

<p>Hydrolysis (C)</p> Signup and view all the answers

What is a major disadvantage of tertiary treatment processes in wastewater management?

<p>They are often costly to implement (A)</p> Signup and view all the answers

Which of the following is NOT a role played by Zoogloea ranigera in wastewater treatment?

<p>Decomposing heavy metals (A)</p> Signup and view all the answers

What is the final product of the methanogenesis stage in anaerobic treatment?

<p>Methane gas (C)</p> Signup and view all the answers

What is the primary goal of aerobic wastewater treatment?

<p>To reduce oxygen demand in the effluent (B)</p> Signup and view all the answers

In anaerobic treatment systems, what is primarily broken down during acidogenesis?

<p>Larger molecules into ammonia and fatty acids (A)</p> Signup and view all the answers

What can excessive phosphorus in water bodies lead to?

<p>Oxygen depletion and algal blooms (B)</p> Signup and view all the answers

During the activated sludge process, the primary role of recirculated sludge is to:

<p>Introduce acclimated microorganisms (B)</p> Signup and view all the answers

What happens to the clear water after secondary treatment?

<p>It is skimmed, disinfected, and discharged (B)</p> Signup and view all the answers

What type of organisms are mainly responsible for treating wastewater in anaerobic systems?

<p>Anaerobic microorganisms (A)</p> Signup and view all the answers

Why is high specific surface area important in trickling filters?

<p>To promote biofilm formation (A)</p> Signup and view all the answers

What is the main disinfection process used to treat public water schemes in Ireland?

<p>Chlorine disinfection (A)</p> Signup and view all the answers

What condition is mono-chloramination particularly used for?

<p>Elevated levels of Trihalomethanes (B)</p> Signup and view all the answers

Which factor does NOT influence the formation of Trihalomethanes (THMs)?

<p>Water temperature in winter (A)</p> Signup and view all the answers

What are Freshwater pearl mussels sensitive to?

<p>Pollution from improperly treated wastewater (B)</p> Signup and view all the answers

What percentage of treated water is lost due to leaks before reaching taps?

<p>37% (B)</p> Signup and view all the answers

Which harmful consequence is related to the presence of Giardia intestinalis in drinking water?

<p>Acute gastroenteritis (A)</p> Signup and view all the answers

What main health risk is associated with elevated levels of Trihalomethanes (THMs)?

<p>Long-term chronic diseases (A)</p> Signup and view all the answers

What is a primary source of waterborne diseases?

<p>Improperly treated potable water (B)</p> Signup and view all the answers

Which condition does NOT lead to increased formation of Trihalomethanes?

<p>Low levels of chlorine (D)</p> Signup and view all the answers

What action must Uisce Éireann undertake after completing improvements in wastewater treatment?

<p>Conduct environmental monitoring (B)</p> Signup and view all the answers

What effect does the presence of Giardia cysts in water have on human health?

<p>Transmission of gastrointestinal infection (D)</p> Signup and view all the answers

Which of the following statements about treatment facility adequacy is true?

<p>Lack of facilities contributes to disease spread (D)</p> Signup and view all the answers

What is a common issue that leads to leaks in treated water systems?

<p>Old and damaged pipes (A)</p> Signup and view all the answers

How is Giardia intestinalis primarily spread?

<p>Contamination of food and water (A)</p> Signup and view all the answers

What product is released when MUG is hydrolyzed by β-glucuronidase?

<p>4-methylumbelliferone (D)</p> Signup and view all the answers

Which test is used to determine the ability of an organism to utilize sodium citrate as its only carbon source?

<p>Citrate utilization test (C)</p> Signup and view all the answers

Which of the following bacteria is typically methyl red positive?

<p>Escherichia coli (B)</p> Signup and view all the answers

What does a positive Voges-Proskauer test indicate about an organism?

<p>It produces acetylmethylcarbinol from glucose fermentation. (B)</p> Signup and view all the answers

Which type of microorganism is tested alongside E.coli in the Colilert test?

<p>Faecal enterococci (A)</p> Signup and view all the answers

What is the outcome of a citrate positive test?

<p>Visibility of growth and an intense Prussian blue color (D)</p> Signup and view all the answers

Which part of IMViC tests stands for the ability to split the amino acid tryptophan?

<p>Indole (B)</p> Signup and view all the answers

What does a yellow color indicate in the Colilert test?

<p>Growth of total coliforms (D)</p> Signup and view all the answers

Which bacteria is typically negative for the Voges-Proskauer test?

<p>Escherichia coli (A)</p> Signup and view all the answers

Which enzyme do coliforms use to metabolize ONPG in the Colilert test?

<p>β-galactosidase (B)</p> Signup and view all the answers

What is the primary method of cholera infection transmission?

<p>Drinking contaminated water (C)</p> Signup and view all the answers

Which treatment is commonly used for cholera?

<p>Antibiotics like fluoroquinolones (C)</p> Signup and view all the answers

What do coliform bacteria indicate in water quality?

<p>Presence of human fecal contamination (A)</p> Signup and view all the answers

Which part of the coliform group is considered a strong indicator for pathogenic bacteria?

<p>Fecal coliforms (B)</p> Signup and view all the answers

What characteristic distinguishes Eosin Methylene Blue (EMB) agar?

<p>Differentiates lactose fermenters by color (D)</p> Signup and view all the answers

What color do total coliform colonies appear on m-Endo medium?

<p>Pink to dark red with metallic sheen (D)</p> Signup and view all the answers

What factors contribute to the presence of pathogenic bacteria in recreational waters?

<p>Urban runoff and sewage systems (A)</p> Signup and view all the answers

How long does it generally take to complete the coliform testing process?

<p>At least 4 days (D)</p> Signup and view all the answers

What is a major characteristic of coliform bacteria?

<p>They ferment lactose with gas production (D)</p> Signup and view all the answers

What does the presence of E. coli in water primarily indicate?

<p>Potential fecal contamination (D)</p> Signup and view all the answers

What type of medium is recommended for the fecal coliform test?

<p>m-FC agar (A)</p> Signup and view all the answers

Which of the following is NOT part of the original coliform testing process?

<p>Quantitative test (A)</p> Signup and view all the answers

Which of the following is true about total coliforms?

<p>They are always present in the digestive tract of animals (D)</p> Signup and view all the answers

What type of bacteria does EMB agar specifically inhibit?

<p>Gram-positive bacteria (B)</p> Signup and view all the answers

What is the primary method of transmission for Cryptosporidium parvum?

<p>Fecally contaminated water (D)</p> Signup and view all the answers

Which treatment method is effective in inactivating Cryptosporidium?

<p>Ultraviolet light (A)</p> Signup and view all the answers

What percentage of the developed world's population is estimated to have giardiasis?

<p>7% (A)</p> Signup and view all the answers

What is a significant factor for Legionella bacteria growth in water?

<p>Water temperature between 20 and 45 degrees Celsius (A)</p> Signup and view all the answers

Which bacteria are commonly associated with Legionnaires' disease?

<p>Legionella (B)</p> Signup and view all the answers

What environment is Vibrio cholerae most commonly found in?

<p>Locations with poor sanitary conditions (C)</p> Signup and view all the answers

What is the primary diagnostic method for giardiasis?

<p>Stool tests (B)</p> Signup and view all the answers

How long can Cryptosporidium oocysts survive in cold water?

<p>3 months (D)</p> Signup and view all the answers

Which of the following is a likely route of infection for Cryptosporidiosis?

<p>Ingestion of contaminated water (D)</p> Signup and view all the answers

What is the main health risk associated with inadequate water supply maintenance?

<p>Increased risk of Legionnaires' disease (C)</p> Signup and view all the answers

Which water treatment method is NOT effective against Cryptosporidium?

<p>Chlorine disinfection (C)</p> Signup and view all the answers

The increase in Cryptosporidiosis cases in Ireland was reported to be what percentage?

<p>65% (D)</p> Signup and view all the answers

What aids in preventing the spread of Legionnaires' disease?

<p>Running water through taps weekly (A)</p> Signup and view all the answers

Flashcards

Water Quality's Impact on Health

The quality of drinking water is a significant factor in determining public health. Access to clean drinking water is crucial for survival and well-being.

Uisce Éireann's Role

Uisce Éireann is responsible for providing public water services and ensuring that drinking water meets quality standards.

EPA's Role in Drinking Water

The EPA, or Environmental Protection Agency, monitors and enforces drinking water regulations for public water supplies.

Local Authorities' Role in Drinking Water

Local authorities are responsible for regulating drinking water for private supplies, including group water schemes and businesses.

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HSE's Role in Drinking Water

The HSE, or Health Service Executive, is involved when there are potential public health risks related to water quality.

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CRU's Role in Drinking Water

The CRU, or Commission for Regulation of Utilities, oversees the financial aspects of water provision, ensuring efficiency and cost-effectiveness.

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EPA's Role in Environmental Protection

The EPA plays a vital role in ensuring a clean and healthy environment by implementing regulations, providing environmental information, and advocating for sustainable practices.

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New Drinking Water Regulations

The new drinking water regulations, passed in 2023, are aimed at water suppliers to enforce stricter quality standards.

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Nutrient pollution

Excessive nutrient pollution, primarily phosphorus, can cause harmful algal blooms in water bodies.

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Why is phosphorus removal difficult?

Phosphorus, a key nutrient, can easily dissolve in water and is difficult to remove once it's there.

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Chemical precipitation

This treatment method involves using iron and aluminum coagulants to remove phosphorus from water.

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Polyphosphate-accumulating organisms (PAOs)

Bacteria that can help remove excess phosphorus from water.

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Biological removal

This method uses bacteria to remove phosphorus from water.

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Physical removal of phosphorus

Physical removal methods, such as sand filtration and membrane technologies, can be used independently or alongside other phosphorus removal methods.

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What does tertiary treatment do?

Wastewater treated by secondary methods is not yet safe for drinking. Several additional treatments are needed.

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What is drinking water purification?

Raw water undergoes purification to remove contaminants and make it safe for drinking.

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Sedimentation

The process of removing solids and other impurities from water by allowing them to settle.

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Coagulation

Adding chemicals like alum to water to form sticky flocs that trap impurities and settle.

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Flocculation

The gentle movement of water to promote the gathering of small flocs into larger masses.

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Filtration

A process of passing water through a sand bed to remove remaining microorganisms and impurities.

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Disinfection

Using disinfectants like chlorine or UV radiation to kill bacteria and other harmful microorganisms in water.

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Aeration

Aeration is the process of introducing air into water to remove dissolved gases and oxidize dissolved metals.

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Storage or settling

Storage tanks allow time for suspended particles to settle and microorganisms to die off.

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European Union (Drinking Water) Regulations 2023

The European Union (Drinking Water) Regulations 2023 are a set of rules enhancing existing requirements to protect human health by setting clear guidelines for safe drinking water management.

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Safe Drinking Water Standards

The regulations ensure that water intended for human consumption is wholesome, clean, and free from harmful substances or micro-organisms.

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Tackling Water Leakages

The regulations address the issue of water leakages during distribution, which can lead to significant waste of treated water, by setting new standards to reduce losses.

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Biochemical Oxygen Demand (BOD)

A measure of the amount of dissolved oxygen consumed by microorganisms in the breakdown of organic matter in wastewater under aerobic conditions. It indicates the organic strength of a wastewater sample.

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Chemical Oxygen Demand (COD)

A test that measures the amount of oxygen required to chemically oxidize the organic and inorganic materials present in water.

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Biological Wastewater Treatment

The biological treatment of wastewater involves the use of microorganisms to break down organic matter.

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Primary Wastewater Treatment

The removal of solid and particulate organic and inorganic materials from wastewater using physical separation methods.

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Secondary Wastewater Treatment

The removal of microorganisms from wastewater through processes like aeration and settling.

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Physical Separation Methods

Physical methods used in primary treatment to remove large, insoluble objects from wastewater. These methods include screening and grit chambers.

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Screens

Long, closely spaced, narrow metal bars used in primary wastewater treatment to block floating debris, preventing clogging of pipes or pumps.

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Grit Chambers

Long, narrow tanks designed to slow down wastewater flow, allowing heavier solids to settle at the bottom for removal.

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Sludge

The settled solids removed from wastewater in primary treatment that undergoes further treatment.

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Flocculants

Chemicals used in wastewater treatment to bind suspended particles together, allowing them to settle more easily.

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Fixed-Film Systems

Wastewater treatment techniques that involve microbial growth on fixed media, like stones or reeds.

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Suspended-growth Systems

Wastewater treatment techniques that involve microbial growth suspended in the water, like in the activated sludge process.

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Secondary treatment- aerobic

A wastewater treatment process where microorganisms break down organic contaminants using oxygen.

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Activated sludge process

A wastewater treatment process where bacteria thrive in a well-oxygenated mixture of sewage and sludge.

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Anaerobic treatment

A biological process in wastewater treatment that removes organic matter using anaerobic microorganisms. These organisms survive and thrive without oxygen.

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Hydrolysis (Anaerobic)

A type of anaerobic treatment where large molecules are broken down into smaller molecules like amino acids and fatty acids.

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Acidoegnesis

A stage in anaerobic treatment where acidogenic bacteria break down molecules, producing acids like ammonia and carbonic acid.

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Acetogenesis

The stage in anaerobic treatment where acetate and acetic acid are formed.

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Methanogenesis

The final stage of anaerobic treatment where methane gas is produced.

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Tertiary treatment

A type of wastewater treatment that focuses on reducing inorganic nutrients like phosphates and nitrates.

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Zoogloea ranigera

A common bacterium used in activated sludge treatment, known for its ability to form flocs and degrade organic matter.

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EPS (Extracellular Polymeric Substances)

Sticky, slimy substances produced by bacteria that help them clump together in flocs.

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Trickling Filter

A type of wastewater treatment that involves trickling pre-treated wastewater over a media bed. This allows microorganisms to break down organic matter.

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Trickling Filter

Large structures used in wastewater treatment that contain media like rocks or plastic to support the growth of microorganisms.

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Biofilm Formation

A biological process involving the breakdown of organic matter by aerobic bacteria, resulting in the formation of carbon dioxide and water.

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Organic Matter Degradation

A type of treatment where bacteria break down organic matter, reducing the biochemical oxygen demand (BOD) and chemical oxygen demand (COD).

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Eutrophication

Excessive phosphorus can lead to algal blooms, which deplete oxygen in water bodies and create dead zones.

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Chlorination in Irish water treatment

Chlorination is a primary water purification method in Ireland, aimed at eliminating harmful microorganisms.

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Mono-chloramination

Mono-chloramination is a water treatment technique that combines chlorine and ammonia to disinfect drinking water. It's especially effective for longer water networks.

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Trihalomethanes (THMs)

Trihalomethanes (THMs) are chemicals produced when chlorine reacts with organic matter in water. They are a potential health concern, albeit managed with careful treatment.

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Organic matter and THM formation

Organic matter in untreated water can lead to the formation of THMs during chlorination.

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Chlorine concentration and THMs

Higher chlorine levels increase the likelihood of THM formation. Balancing disinfection and minimizing THMs is crucial.

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Time and THM formation

The longer water sits in pipes, the greater the chance for THMs to form. Prompt delivery helps minimize their formation.

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Seasonal variation in THMs

Autumn often sees increased THM formation due to decaying leaves entering water sources. This is linked to higher water temperatures.

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Urban Waste Water Treatment Directive

The Urban Waste Water Treatment Directive sets standards for wastewater treatment in large urban areas to protect the environment.

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Compliance with Waste Water Standards

In 2023, out of 177 large urban areas in Ireland, 166 complied with the standards set by the Urban Waste Water Treatment Directive.

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Raw sewage discharge

In 2023, 26 towns and villages in Ireland were discharging raw sewage, posing a significant risk to the environment.

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Wastewater treatment and pearl mussels

The EPA identified 12 towns and villages where improved wastewater treatment is needed to protect freshwater pearl mussels.

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Freshwater pearl mussels

Freshwater pearl mussels are endangered, requiring clean, well-oxygenated rivers. Pollution from wastewater harms their survival.

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Uisce Éireann's responsibilities

Uisce Éireann needs to prioritize improvement works in areas with endangered freshwater pearl mussels.

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Water leakage in Ireland

Leakage in Ireland's water network is a major issue. About 37% of treated water is lost before reaching taps.

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Addressing water leakage

Ongoing efforts are being made to reduce water leakage in Ireland. The national target is to achieve a leakage rate of 25% by 2030.

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Colilert Test

A test that uses the substrate MUG (4-methylumbelliferyl-β-D-glucuronide) to detect the presence of coliforms. Coliforms produce β-glucuronidase, which hydrolyzes MUG, releasing a fluorescent product detectable under UV light.

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Coliforms

A group of bacteria commonly found in the intestines of humans and animals. Their presence in water can indicate fecal contamination.

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E. coli

A specific type of coliform bacteria, commonly associated with fecal contamination and potentially harmful to human health.

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IMViC Tests

A series of biochemical tests used to differentiate between different species of Enterobacteriaceae. It stands for Indole, Methyl Red, Voges-Proskauer, and Citrate.

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Indole Test

A test that measures the production of indole from the amino acid tryptophan. Used to differentiate between Enterobacteriaceae.

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Methyl Red (MR) Test

A test that determines if a bacterial species produces stable acids during mixed acid fermentation. Methyl red is the pH indicator used for this test.

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Voges-Proskauer (VP) Test

A test that assesses if a bacterial species produces acetoin (acetylmethylcarbinol) from glucose fermentation.

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Citrate Utilization Test

A test that determines the ability of bacteria to utilize citrate as their sole carbon source and ammonium dihydrogen phosphate as their nitrogen source.

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Enterobacteriaceae

A large family of gram-negative, rod-shaped bacteria, including common bacteria like E. coli, Salmonella, and Shigella.

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Water Purification

The process of removing or reducing harmful substances or microorganisms from water to make it safe for drinking.

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Giardia

A parasitic protist that causes giardiasis, a common intestinal infection. It's spread through contaminated water or food.

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Giardiasis

An infection caused by Giardia parasites, leading to diarrhea, abdominal cramps, bloating, and fatigue.

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Cryptosporidium parvum

A protist that causes cryptosporidiosis, an intestinal infection. It's highly resistant to chlorine, making it difficult to treat.

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Cryptosporidiosis

An infection caused by Cryptosporidium parvum, resulting in diarrhea, stomach cramps, and fever. It can be more severe in immunocompromised individuals.

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Cryptosporidium Removal

The most effective methods to remove Cryptosporidium oocysts from water are sedimentation and filtration.

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Legionnaires' disease

A serious, sometimes fatal pneumonia caused by Legionella bacteria. It's usually contracted by inhaling contaminated water droplets.

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Pontiac fever

A mild, flu-like illness caused by Legionella bacteria, usually self-limiting.

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Preventing Legionnaires' Disease

The primary way to prevent Legionnaires' disease is by ensuring proper care of water systems where Legionella bacteria can grow.

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Cholera

A highly contagious diarrheal disease caused by Vibrio cholerae bacteria. It's a major public health problem in areas with poor sanitation.

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Vibrio cholerae

The bacteria responsible for causing cholera, a severe diarrheal disease affecting millions globally.

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Buffered Charcoal-Yeast Extract (BCYE) Medium

A specialized medium used for growing Legionella bacteria, containing yeast extract, iron, L-cysteine, and activated charcoal.

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Facultative intracellular parasite

A specific type of bacteria that infects alveolar macrophages during Legionnaires' disease.

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Complex Nutritional Requirements

A unique characteristic of Legionella bacteria, benefiting from L-cysteine for growth and using amino acids for energy.

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L-cysteine

The primary growth factor required for Legionella bacteria, an amino acid important for its survival.

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Ferric iron

One of the essential nutrients required for Legionella bacteria growth, along with L-cysteine.

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What are coliforms?

A group of bacteria that are commonly found in the digestive tract of animals, including humans. They are usually harmless, but can indicate fecal contamination in water.

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Coliforms are...

A group of bacteria that are commonly found in the digestive tract of animals, including humans. They are usually harmless, but can indicate fecal contamination in water. They are:

  • Relatively easy to identify.
  • Present in larger numbers than more dangerous pathogens.
  • Respond to the environment, wastewater treatment and water treatment similarly to pathogens.
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Indicator Organisms

Bacteria that are used to assess the quality of water and other environments. Their presence or absence can indicate the presence of harmful pathogens that could cause illness.

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EMB Agar

A type of agar that is both selective and differential, meaning it inhibits the growth of certain bacteria and distinguishes between others based on their characteristics. Commonly used for isolating faecal coliforms.

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Eosin Methylene Blue (EMB) agar

A type of agar that is selective because it inhibits the growth of gram-positive bacteria, and differential because it distinguishes between organisms that ferment lactose (e.g., E.coli) and those that do not (e.g., Salmonella, Shigella).

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Membrane Filtration

A technique used to evaluate the microbiological characteristics of water. The water sample is passed through a membrane filter, which traps bacteria on its surface. The filter is then transferred to a solid medium or liquid medium to detect the presence of coliforms, fecal coliforms, or enterococci.

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m-Endo media

A type of media used in membrane filtration for the detection of total coliforms. Lactose fermenting organisms form red coloured zones around colonies with a metallic sheen.

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mFC Agar

A type of agar used in membrane filtration for the detection of faecal coliforms. Methyl blue suppresses gram-positive bacteria growth and coliforms form blue colonies.

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Membrane Filtration

A common and preferred method of evaluating the microbiological characteristics of water. The water sample is passed through a membrane filter, which traps bacteria that are larger than 0.45μm in diameter.

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Defined Substrate tests

A type of test that uses defined substrates (chemicals) that are broken down by specific enzymes produced by bacteria. This allows for the detection and quantification of coliforms and E.coli.

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IDEXX Colilert

A commercial product that uses defined substrates for the detection and quantification of coliforms and E.coli. It uses two nutrient indicators, ONPG and MUG, which are metabolised by specific enzymes.

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Beta-glucuronidase

An enzyme produced by E.coli and other bacteria, including Salmonella, Shigella, and Yersinia. It is used in defined substrate tests to detect the presence of fecal coliforms.

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Study Notes

Water Quality and Waterborne Diseases

  • Drinking water quality is a crucial environmental factor affecting health, supporting life and socio-economic development.
  • Uisce Éireann manages public water supplies; the EPA regulates drinking water quality for public schemes; Local Authorities regulate private supplies.
  • The HSE handles public health concerns related to drinking water and must be consulted in cases of non-compliance or public health risks; CRU regulates Uisce Éireann economically.
  • The EPA regulates and monitors the environment, providing data and knowledge for decision-making.

New Drinking Water Regulations (2023)

  • The European Union (Drinking Water) Regulations 2023 (S.I. No. 99/2023) mandate water intended for human consumption to be wholesome, clean, and risk-free, meeting regulation requirements.
  • The regulations outline detailed directions for managing water for human consumption, particularly for marginalized groups.
  • These regulations target water suppliers (Uisce Éireann and group water schemes) and are enforced by relevant authorities.
  • Water must be free of harmful microorganisms and substances, and meeting quality standards outlined in Schedules A, B, and D is crucial.

Drinking Water Quality Standards (Tables A and B)

  • Table A (Microbiological):
    • Intestinal enterococci and Escherichia coli (E.coli) levels must be zero (or zero per 250 ml for bottled water).
  • Table B (Chemical):
    • Specific parametric values are set for chemical substances like Acrylamide (0.10 µg/L), Antimony (10 µg/L), Arsenic (10 µg/L), Benzene (1.0 µg/L), and Benzo(a)pyrene (0.010 µg/L).

Biochemical Oxygen Demand (BOD) and Chemical Oxygen Demand (COD)

  • BOD measures the rate at which microorganisms break down organic matter in wastewater.
  • BOD5 measures the dissolved oxygen reduction after 5 days of incubation at 20°C.
  • COD measures the oxygen needed to oxidize organic matter and other nutrients in water.
  • Key wastewater characteristics like COD, BOD5, Suspended Solids, Ammonia, and Phosphorus are quantified, typically with ranges presented in mg/L.

Wastewater and Sewage Treatment

  • Wastewater treatment aims to reduce organic and inorganic materials to non-microbial levels.
  • Measures the amount of dissolved oxygen used by microbes to completely oxidize matter in a water sample to assess efficiency.
  • Wastewater treatment uses physical and biological methods for treatment.

Primary Treatment

  • Screening: Removes large floating solids.
  • Grit Chamber: Slows flow to settle out sand and gravel.
  • Sedimentation: Removes solids that sink by gravity.
  • Flocculation: Uses chemicals to aggregate suspended particles.

Secondary Treatment

  • Aerobic Processes: Adding air oxidizes organic matter through aerobic biological degradation, using activated sludge or trickling filters.
  • Trickling Filters: Wastewater trickles through media (e.g., rock), supporting biofilm growth and organic matter oxidation.
  • Activated Sludge Process: Wastewater mixes with microorganisms for organic breakdown, followed by sludge separation and reuse.
  • Zoogloea ranigera: A bacterium producing EPS, which aids in floc formation and efficient organic matter degradation in wastewater.

Tertiary Treatment

  • Tertiary treatment further purifies water for drinking standards.
  • Different methods for reducing levels of inorganic elements like phosphorus (e.g. chemical precipitation, biological removal, physical removal).

Drinking Water Purification

  • Multi-step process for making raw water safe to drink.
  • Aeration: Removes gases, oxidizes metals (e.g., iron).
  • Storage/Settling: Allows suspended solids and certain chemicals to settle for removal.
  • Coagulation: Uses chemical precipitating agents to form heavier flocs.
  • Flocculation: Promotes aggregate formation of fine flocs for efficient removal.
  • Filtration: Removes microorganisms and impurities.
  • Disinfection: Kills pathogens using disinfectants (chlorine, chlorine dioxide, ozone, etc.).

THMs (Trihalomethanes)

  • THMs are formed when chlorine reacts with organic matter in water, and levels can be a public health concern, although the HSE finds benefits exceed potential health risks.
  • Formation is affected by source water's organic matter, chlorine levels, time, pipe network length, and weather conditions.

Urban Wastewater Treatment

  • The Urban Waste Water Treatment Directive aims to protect the environment from untreated wastewater from 177 large urban areas.
  • In 2023, 166 areas complied with the standards.
  • 12 towns and villages require Uisce Éireann improvements to safeguard freshwater pearl mussels.

Waterborne Disease Sources

  • Waterborne illnesses can come from contaminated drinking water, non-regulated sources (wells), recreational water, and inadequate hygiene.

Giardiasis and Cryptosporidiosis

  • Giardiasis: caused by Giardia intestinalis, transmitted in fecally contaminated water.
  • Cryptosporidiosis: caused by Cryptosporidium parvum, transmitted in fecally contaminated water. Both are highly resistant to common disinfectants.

Legionnaires' Disease

  • Caused by Legionella bacteria, transmitted by inhaling water droplets.
  • Growth best in water temperatures between 20 and 45°C.

Cholera

  • Caused by Vibrio cholerae, transmitted mainly via contaminated water.

Indicator Organisms

  • Indicator organisms like total coliforms, fecal coliforms, E. coli, and enterococci are used to monitor water quality.
  • Detection for coliforms uses tests like a presumptive, confirmed, and complete steps, needing several days for full results

Water Testing Methods

  • Membrane Filtration: Traps bacteria on a filter for subsequent analysis.
  • Defined Substrate Tests (e.g., Colilert): Quickly detect coliforms and E. coli using color changes or fluorescent reactions.
  • EMB (Eosin Methylene Blue) agar: Selective for Gram-negative bacteria and differentiates between lactose-fermenting and non-fermenting organisms. Different media, like mENDO and mFC, aid in specific bacterium identification based on color (e.g., red with sheen for coliforms, blue for faecal coliforms).
  • IMViC tests: further testing (e.g., Indole, Methyl Red, Voges-Proskauer, Citrate tests) differentiate between bacteria in the Enterobacteriaceae family (e.g. Escherichia coli and others).

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