Enumeration of Microorganisms in Foods

False (B)

<p>Colony crowding and spreaders can make accurate enumeration difficult.</p> Signup and view all the answers

<p>False (B)</p> Signup and view all the answers

<p>Most probable number (MPN)</p> Signup and view all the answers

<p>DMC counts both viable and non-viable cells.</p> Signup and view all the answers

<p>Pour Plate Method = Suitable for heat-sensitive organisms Spread Plate Method = Provides visual features for presumptive identification Most Probable Number (MPN) Technique = Requires a large number of glassware</p> Signup and view all the answers

<p>low</p> Signup and view all the answers

<p>Direct Microscopic Count (DMC) = Rapid and easy enumeration Membrane filter method = Suitable for samples with low bacterial counts Culture-based method = Differentiates viable from non-viable microbes</p> Signup and view all the answers

<p>Provides accurate enumeration of only viable cells (A)</p> Signup and view all the answers

<p>True (A)</p> Signup and view all the answers

<p>Culturing microorganisms in a suitable media to allow them to multiply and form visible colonies, which are then counted to estimate the microbial load.</p> Signup and view all the answers

<p>Type of disinfectant used (B)</p> Signup and view all the answers

<p>True (A)</p> Signup and view all the answers

<p>The principle of SPC is that each viable bacterial cell multiplies and grows into a visible colony. Counting these colonies provides an estimate of the bacterial cells present in a sample.</p> Signup and view all the answers

<p>To determine the presence of specific microorganisms, the types of microorganisms, their number, and their products. (D)</p> Signup and view all the answers

<p>True (A)</p> Signup and view all the answers

<p>pour plate</p> Signup and view all the answers

<p>Direct counting methods and culture-based methods</p> Signup and view all the answers

<p>Baird Parker agar = Staphylococcus Bismuth sulphite agar = Salmonella TCBS = Vibrios Lactose broth = Escherichia coli</p> Signup and view all the answers

<p>Direct Microscopic Count (DMC)</p> Signup and view all the answers

<p>Plate count agar (B)</p> Signup and view all the answers

<p>False (B)</p> Signup and view all the answers

<p>Direct Microscopic Count (DMC) = Counting microorganisms directly by observing a smear under a microscope. Pour Plate = A culture-based method where microorganisms are mixed with melted agar and poured into Petri dishes. Spread Plate = A culture-based method where a diluted sample is spread onto the surface of an agar plate. Most Probable Number (MPN) = Estimating the number of microorganisms based on the probability of their presence in serial dilutions.</p> Signup and view all the answers

<p>Dye Reduction Test (A)</p> Signup and view all the answers

<p>30-300 CFU</p> Signup and view all the answers

<p>False (B)</p> Signup and view all the answers

<p>It can only be used when microorganisms are densely populated, typically at least 10^6 per ml.</p> Signup and view all the answers

Analyzing foods for the presence of microorganisms.

Determining how long food is safe to consume based on microbiological quality.

Counting microorganisms directly or their colonies in food samples.

Counting microorganisms by observing them under a microscope.

Counting microorganisms by allowing them to grow into colonies.

A method of estimating the number of viable microorganisms in a sample.

Estimating microorganism numbers based on their products rather than counting them directly.

Techniques for quick microbial enumeration, including immunological and DNA methodologies.

A technique where diluted sample is spread over the surface of solid agar plates for counting colonies.

It allows studying heat-sensitive psychrotrophs and provides colony features for identification.

Challenges include spreaders and colony crowding which hinder accurate enumeration.

Simple, standardized results, suitable for low numbers and specific organisms, often used for coliform detection.

The evaluation of microbial contamination in food, especially milk.

Dyes used to enhance detection of microbes under a microscope.

A technique using filters to concentrate and count bacteria from a sample.

Includes rapid enumeration and assessment of cell morphology.

Includes tedious counting and miscounting food particles as cells.

Involving growing microorganisms on media to estimate their quantity.

Uses fluorescent dyes to differentiate viable from non-viable cells.

Techniques to enumerate viable microorganisms using culture media.

Culture media designed to grow specific types of microorganisms while inhibiting others.

A method to estimate the number of viable cells in a sample by counting colonies on agar plates.

Units used to estimate the number of viable bacterial cells in a sample based on colony count.

A technique where a diluted sample is mixed with agar and poured into a plate to develop colonies.

Variables that can influence the accuracy of SPC results, such as media and temperature.

Culture media that supports the growth of a wide range of microorganisms.

Environmental settings like temperature and time that affect microbial growth in culture media.