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Entamoeba histolytica Classification Quiz

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Entamoeba histolytica Classification Quiz

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Questions and Answers

Which characteristic distinguishes the species of Entamoeba except E.histolytica, E.dispar, and E.moshkovskii?

Presence of a vesicular nucleus

Morphologically identical appearance (correct)

Varying numbers of chromatin granules adhering to the nuclear membrane

Centrally located small karyosome

How were the three morphologically identical species of Entamoeba differentiated?

Size comparison under electron microscope

PCR restriction fragment length polymorphism (RFLP) (correct)

Microscopic examination of nuclear membrane

Testing for flagella presence

What is the primary basis for differentiating E.hartmanni from other species like the 'small race' of E.histolytica?

Isoenzyme analysis

Small size (correct)

Thermostable DNA polymerase

DNA primers designed specifically for the region of interest

Which technique involves making many copies of a specific DNA region in a test tube?

PCR (C) Signup and view all the answers

What type of parasite is Entamoeba histolytica?

Non-flagellated protozoan (B) Signup and view all the answers

Which component is required for PCR to make many copies of a specific DNA region?

DNA primers (B) Signup and view all the answers

How does Entamoeba histolytica multiply?

By binary fission (C) Signup and view all the answers

What is the size range of E.histolytica trophozoites?

12 to 60 μm (B) Signup and view all the answers

Where are cysts typically found?

In diarrheal stool (B) Signup and view all the answers

What is the main mode of transmission of E.histolytica?

Through ingestion of mature cysts (D) Signup and view all the answers

What happens during excystation of E.histolytica?

Formation of trophozoites (C) Signup and view all the answers

What are the characteristics of E.histolytica cysts?

Highly refractile hyaline cyst wall, quadranucleate, and rod-shaped chromatoidal bars (C) Signup and view all the answers