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Questions and Answers
What is the focus of the anatomical method of hormonal assay?
What is the focus of the anatomical method of hormonal assay?
- Examining the site and structure of the endocrine gland (correct)
- Extracting hormone from blood
- Determining the chemical structure of hormones
- Studying the effects of gland removal on physiology
Which method involves injecting a gland extract to observe the development of an atrophied organ?
Which method involves injecting a gland extract to observe the development of an atrophied organ?
- Replacement or substitution method (correct)
- Surgical removal method
- Injection of antisera of specific hormone method
- Isolation of active hormone method
What is the purpose of the isolation of active hormone method?
What is the purpose of the isolation of active hormone method?
- To analyze the physiological effects of hormone absence
- To replace removed glands with substitutes
- To extract and purify hormones for chemical analysis (correct)
- To determine hormone concentration in the body
How does the surgical removal method function in hormonal assays?
How does the surgical removal method function in hormonal assays?
Why is the injection of antisera of specific hormone method important?
Why is the injection of antisera of specific hormone method important?
What physiological change occurs in a cock after the removal of its testes, as studied in hormonal assays?
What physiological change occurs in a cock after the removal of its testes, as studied in hormonal assays?
Which method would likely involve histological examination of the endocrine gland?
Which method would likely involve histological examination of the endocrine gland?
What function does the replacement or substitution method serve in hormonal assays?
What function does the replacement or substitution method serve in hormonal assays?
What is the primary purpose of the injection of antisera of specific hormone method?
What is the primary purpose of the injection of antisera of specific hormone method?
In the replacement or substitution method, what occurs when gland extract is injected into an animal with a surgically removed gland?
In the replacement or substitution method, what occurs when gland extract is injected into an animal with a surgically removed gland?
What is the process involved in the isolation of active hormone method?
What is the process involved in the isolation of active hormone method?
What is the fundamental principle of immunological methods such as immunoassays?
What is the fundamental principle of immunological methods such as immunoassays?
Which of the following best describes an immunoassay?
Which of the following best describes an immunoassay?
What can be expected to happen if an animal has a gland surgically removed and receives no gland extract?
What can be expected to happen if an animal has a gland surgically removed and receives no gland extract?
What role do antibodies play in immunoassays?
What role do antibodies play in immunoassays?
How is the hormone concentration determined using antisera?
How is the hormone concentration determined using antisera?
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Study Notes
Anatomical Method
- Involves examining endocrine glands microscopically and macroscopically.
- Identifies the gland's location and structure within the animal's body.
Surgical Removal Method
- Involves removing the endocrine gland and observing the physiological effects of its absence.
- For instance, removing a rooster's testes leads to regression of its comb, wattles, and ear lobes.
Replacement or Substitution Method
- Involves injecting the gland extract into an animal where the gland has been surgically removed.
- Rejuvenates the atrophied organs, highlighting the importance of the extracted hormone in organ development.
Isolation of Active Hormone Method
- Relies on extracting and purifying the hormone from the gland or blood.
- The purified hormone is then analyzed to determine its chemical structure and confirm its physiological activity.
Injection of Antisera of Specific Hormone Method
- Crucial for determining hormone concentration.
Immunological Methods (Immunoassay)
- A test that utilizes antibody-antigen complexities to generate measurable results.
- An analytical method that employs antibodies to quantify specific analytes.
- Based on the principle of antigen-antibody binding.
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