Embryology Quiz on Germ Cells and Development
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Questions and Answers

What is the immediate precursor to the blastocyst that travels through the fallopian tube?

  • Zygote (correct)
  • Primordial germ cell
  • Primary oocyte
  • Oogonia
  • From which embryonic layer do the primordial germ cells originate?

  • Endoderm
  • Trophoblast
  • Mesoderm
  • Epiblast (correct)
  • Where do primordial germ cells migrate to after leaving the yolk sac?

  • Inner cell mass
  • Endometrium
  • Genital ridge (correct)
  • Fallopian tube
  • What is the ploidy of the primordial germ cells once they reach the genital ridge?

    <p>Diploid (C)</p> Signup and view all the answers

    What is the term for the cells that differentiate into mature oocytes?

    <p>Oogonia (C)</p> Signup and view all the answers

    When do the primary oocytes start prophase of meiosis I?

    <p>Before birth (C)</p> Signup and view all the answers

    What arrests the primary oocytes in prophase-1?

    <p>The presence of flat folicular epithelial cells (D)</p> Signup and view all the answers

    What is the approximate number of primary oocytes present at birth?

    <p>600,000 to 800,000 (B)</p> Signup and view all the answers

    Around which day does the cranial neuropore typically close during development?

    <p>Day 25 (B)</p> Signup and view all the answers

    What is the term for the developmental defect that occurs when the neural tube fails to close in the cranial region?

    <p>Anencephaly (A)</p> Signup and view all the answers

    What is the most common location for spina bifida to occur?

    <p>Lumbosacral region (D)</p> Signup and view all the answers

    What is the maternal component of the placenta called?

    <p>Decidua basalis (C)</p> Signup and view all the answers

    Which of the following is NOT considered a type of decidua?

    <p>Decidua spongiosa (D)</p> Signup and view all the answers

    What is the fetal portion of the placenta called?

    <p>Chorion frondosum (A)</p> Signup and view all the answers

    What role does the decidua capsularis play?

    <p>Separates embryo from the uterine lumen (B)</p> Signup and view all the answers

    Which of the following best describes the decidua parietalis?

    <p>The part of the decidua that lines the rest of the uterine cavity (D)</p> Signup and view all the answers

    What is the primary functional unit of the placenta?

    <p>Chorionic villi (C)</p> Signup and view all the answers

    The chorion frondosum is best described as:

    <p>The part of the chorion that forms the placenta due to villi proliferation related to decidua basalis. (C)</p> Signup and view all the answers

    What is the most common type of twins, accounting for approximately 90% of twin pregnancies?

    <p>Dizygotic twins (D)</p> Signup and view all the answers

    Which of the following is NOT true regarding dizygotic twins?

    <p>They always share a single placenta. (A)</p> Signup and view all the answers

    Monozygotic twins result from:

    <p>Fertilization of one secondary oocyte by one sperm. (B)</p> Signup and view all the answers

    If a zygote splits at the two-cell stage, how many placentas, chorionic sacs, and amniotic sacs are typically formed?

    <p>2 placentas, 2 chorionic sacs, 2 amniotic sacs (B)</p> Signup and view all the answers

    What is the primary role of the oocyte maturation inhibitor (OMI) during oogenesis?

    <p>To maintain oocytes in an arrested state (D)</p> Signup and view all the answers

    Approximately how many oocytes remain in the ovaries of a female by puberty?

    <p>40,000 (C)</p> Signup and view all the answers

    What is the term for a twin that dies in the first trimester and becomes compressed and mummified?

    <p>Fetus papyraceus (A)</p> Signup and view all the answers

    Which of the following best describes the antral stage of follicle development?

    <p>The longest stage in follicle maturation, characterized by fluid accumulation. (C)</p> Signup and view all the answers

    A vanishing twin is most often caused by:

    <p>Twin transfusion syndrome or resorption (D)</p> Signup and view all the answers

    What event triggers the completion of meiosis II in an oocyte?

    <p>The entry of the sperm's pronucleus into the oocyte (C)</p> Signup and view all the answers

    What is the main role of the gonadotropin-releasing hormone (GnRH) in the ovarian cycle?

    <p>To induce the release of FSH and LH from the pituitary gland. (A)</p> Signup and view all the answers

    How many viable mature oocytes and polar bodies are produced upon the completion of oogenesis?

    <p>One viable oocyte and three polar bodies (D)</p> Signup and view all the answers

    During the zygote formation process, what happens after the sperm nucleus enters the oocyte?

    <p>Both male and female pronuclei come together and merge to restore the diploid number of chromosomes. (A)</p> Signup and view all the answers

    Approximately how many hours before ovulation does an oocyte arrest in metaphase II?

    <p>3 hours (B)</p> Signup and view all the answers

    Which of the following is NOT a potential cause of clubfoot?

    <p>Amniotic band constrictions (D)</p> Signup and view all the answers

    What is a key characteristic that differentiates eclampsia from preeclampsia?

    <p>Seizures (A)</p> Signup and view all the answers

    Amniotic bands are theorized to originate from which of the following?

    <p>Tears in the amnion (D)</p> Signup and view all the answers

    Which of these conditions is NOT directly associated with preeclampsia?

    <p>Maternal seizures (D)</p> Signup and view all the answers

    Preeclampsia is typically observed starting around which gestational age?

    <p>20 weeks (C)</p> Signup and view all the answers

    Twin-to-twin transfusion syndrome is most likely to occur in which type of pregnancy?

    <p>Monozygotic twins with a shared placenta (C)</p> Signup and view all the answers

    The erector spinae muscles belong to which group of muscles based on developmental origin?

    <p>Epaxial muscles (B)</p> Signup and view all the answers

    Which of the following best describes the muscle group that includes the rectus abdominis?

    <p>Abaxial muscles (D)</p> Signup and view all the answers

    What is the term for the complete absence of a limb?

    <p>Amelia (B)</p> Signup and view all the answers

    Which limb defect is characterized by abnormally short limbs, but with all segments present?

    <p>Micromelia (C)</p> Signup and view all the answers

    Syndactyly is a condition involving which of the following?

    <p>Fusion of two or more digits (D)</p> Signup and view all the answers

    Polydactyly is associated with an abnormal duplication of which structure?

    <p>Zone of polarizing activity (ZPA) (B)</p> Signup and view all the answers

    Cleft hand and foot typically involve the absence of which metacarpal and phalangeal bones?

    <p>Third metacarpal and phalangeal bones (B)</p> Signup and view all the answers

    Flashcards

    Oogenesis

    The process in which oogonia develop into mature oocytes. It begins before birth and involves several mitotic divisions, resulting in a large pool of oogonia.

    Primordial Germ Cells (PGCs)

    Specialized cells that are destined to become germ cells. They originate in the epiblast and migrate to the yolk sac, waiting for a signal to activate.

    Mitosis

    The process of cell division that results in two identical daughter cells. It occurs in oogonia during the early stages of oogenesis.

    Prophase of Meiosis I

    A stage in the cell cycle where the cell prepares to divide. During oogenesis, the majority of oogonia enter this stage.

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    Follicle

    A structure consisting of a primary oocyte surrounded by a layer of flat epithelial cells. These structures are found in the ovary.

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    Puberty

    The time in a female's life when reproductive organs become functional, usually between ages 8 and 13. It is marked by the onset of menstruation. During puberty, primary oocytes complete their meiotic division and enter the next phase of oogenesis.

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    Primary Oocyte Arrest

    A primary oocyte is arrested in prophase I of meiosis until puberty, when hormones stimulate it to continue developing.

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    Number of Primary Oocytes at Birth

    Primary oocytes are estimated to number between 600,000 and 800,000 at birth, but many degenerate over time.

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    What is OMI?

    Oocyte maturation inhibitor (OMI) is a small peptide secreted by follicular cells. It arrests oocyte development at a specific stage.

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    What happens to most oocytes during childhood?

    During childhood, most oocytes undergo atresia - a process of degeneration. Only a small number (~40,000) remain by puberty.

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    Describe the process of follicle maturation at puberty.

    At puberty, each month, 15-20 follicles are selected to mature. Some die, while others enter the antral stage and accumulate fluid.

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    What is a Graafian follicle?

    The mature vesicular follicle (Graafian follicle) is the stage just before ovulation. It lasts about 37 hours.

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    Describe the oocyte's state before ovulation.

    The oocyte enters meiosis II and arrests in metaphase, approximately 3 hours before ovulation.

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    When is meiosis II completed?

    Meiosis II is completed only if fertilization occurs. Without fertilization, the oocyte degenerates within 24 hours after ovulation.

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    What is the final product of oogenesis?

    At the completion of oogenesis (meiosis II), a mature oocyte and one polar body are produced.

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    Describe the pronuclei during fertilization.

    The female pronucleus (23 chromosomes) is present in the definitive oocyte. The male pronucleus (23 chromosomes) enters the oocyte after sperm tail detachment.

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    What is Neurulation?

    The process by which the neural tube, the precursor to the central nervous system, closes. It begins with the formation of the neural plate, which folds inwards and eventually fuses to create the tube.

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    What are Neural Tube Defects?

    A birth defect that occurs when the neural tube fails to close completely during pregnancy. This can lead to various conditions affecting the brain, spinal cord, and other parts of the body.

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    What is Anencephaly?

    A type of neural tube defect where the brain doesn't fully develop. It's characterized by the absence of a portion of the skull and brain.

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    What is Spina Bifida?

    A neural tube defect where the spinal cord doesn't fully close, causing varying degrees of disability. This can affect the ability to walk, control bladder and bowel function, and other bodily functions.

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    What is the Placenta?

    A temporary organ that develops during pregnancy. It attaches to the mother's uterus and provides oxygen and nutrients to the growing fetus.

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    What is the Decidua Basalis?

    The part of the placenta contributed by the mother. It's a portion of the uterine lining where the placenta develops.

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    What is the Chorion Frondosum?

    The part of the placenta contributed by the developing fetus. It's characterized by finger-like projections that increase the surface area for exchange of nutrients and oxygen.

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    What is the Decidua?

    The layer of the uterus that lines the cavity. It is transformed into the decidua after implantation.

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    What is a chorionic villus in the placenta?

    The functional unit of the placenta, formed from the chorionic villi that develop from the chorion frondosum and interact with the decidua basalis.

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    What is chorion frondosum?

    The part of the chorion that develops into the placenta, containing chorionic villi.

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    What is chorion laeve?

    The smooth part of the chorion that doesn't contribute to the placenta.

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    What are dizygotic twins?

    Twins that result from the fertilization of two separate eggs by two different sperm.

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    What are monozygotic twins?

    Twins that result from the fertilization of one egg by one sperm, followed by the splitting of the resulting zygote.

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    What happens when a zygote splits during the two-cell stage?

    The process where a splitting zygote results in two placentas, two chorionic sacs, and two amniotic sacs.

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    What happens when a zygote splits during the early blastocyst stage?

    The process where a splitting zygote results in one placenta, two chorionic sacs, and two amniotic sacs.

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    What are vanishing twins?

    Condition where one twin dies and is absorbed or mummifies within the uterus.

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    Preeclampsia

    A condition during pregnancy characterized by high blood pressure and protein in the urine, caused by reduced blood flow to organs.

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    Eclampsia

    A potentially life-threatening complication of preeclampsia where seizures occur due to high blood pressure.

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    Limb Defects

    A group of birth defects characterized by missing or malformed limbs, which can be caused by various factors.

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    Amniotic Bands

    Abnormal bands of tissue that can wrap around a fetus during development, leading to limb constrictions or amputations.

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    Arthrogryposis

    A condition that can cause clubfoot and is characterized by multiple joint contractures, usually present at birth.

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    Twin Transfusion Syndrome

    A condition where identical twins share a placenta and blood vessels connect them, leading to an uneven distribution of blood flow. One twin may receive more blood than the other.

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    Developmental Origin of Muscles

    The process of muscles forming from specific regions of the somites during embryonic development.

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    Amelia

    A birth defect characterized by the complete absence of one or more limbs.

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    Meromelia

    A birth defect characterized by the partial absence of one or more limbs.

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    Micromelia

    A birth defect characterized by abnormally short limbs, with all segments present.

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    Brachydactyly

    A birth defect characterized by shortened digits (fingers and toes).

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    Syndactyly

    A birth defect characterized by fusion of two or more fingers or toes.

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    Polydactyly

    A birth defect characterized by the presence of extra fingers or toes.

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    Cleft Hand/Foot

    A birth defect characterized by an abnormal cleft between the second and fourth metacarpal/metatarsal bones and soft tissues, often with missing third metacarpal/metatarsal bones.

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    Study Notes

    Review Block-1 Lectures

    • Fall Semester 2024, taught by Dr. Ismail Memon, MBBS, PhD at Saba University School of Medicine.

    Important Syndromes Due to Numerical Chromosomal Abnormalities

    • Trisomy of Autosomes:
      • Trisomy 21 (Down syndrome)
      • Trisomy 18 (Edwards syndrome)
      • Trisomy 13 (Patau syndrome)
    • Trisomy of Sex Chromosomes:
      • Klinefelter syndrome (47, XXY)
      • Triple X syndrome (47, XXX)
    • Monosomy:
      • Turner syndrome (45, XO)

    Klinefelter Syndrome

    • A genetic condition affecting males.
    • Results from an extra X chromosome.
    • Usually diagnosed in adulthood.
    • Characterized by:
      • Sterility
      • Testicular atrophy
      • Hyalinization of seminiferous tubules
      • Longer limbs
      • Gynecomastia

    Triple X Syndrome

    • Affects approximately 1 in 1,000 females.
    • Caused by an extra X chromosome.
    • Often asymptomatic or has mild symptoms.
    • Associated with:
      • Infantile features
      • Scanty menses
      • Some degree of mental retardation

    Turner Syndrome

    • A monosomy condition affecting females.
    • Results from a missing X chromosome.
    • The only monosomy compatible with life.
    • High spontaneous abortion rate (98%).
    • Characterized by:
      • Female phenotype
      • Absence of ovaries
      • Short stature
      • Other associated abnormalities (webbed neck, lymphedema)

    Cri-du-chat Syndrome

    • Caused by a partial deletion of the short arm of chromosome 5.
    • Affected children exhibit:
      • A cat-like cry
      • Microcephaly
      • Mental retardation
      • Congenital heart disease

    Microdeletion Syndrome

    • Angelman Syndrome (maternal chromosome 15):
      • Mental retardation
      • Inability to speak
      • Poor motor development
      • Unprovoked laughter
    • Prader-Willi Syndrome (paternal chromosome 15):
      • Hypotonia
      • Obesity
      • Mental retardation

    Fragile X Syndrome

    • Caused by a mutation in the FMR1 gene (fragile X mental retardation 1).
    • Characterized by:
      • Mental retardation
      • Large ears
      • Prominent jaw
      • Pale blue irises

    Primordial Germ Cells (PGCs)

    • Precursors of sperm and eggs.
    • Generate new generations through germ cells.
    • Migrate and differentiate to form male and female gametes.

    Gametogenesis

    • Process of gamete development.
    • Sperm fertilizes ovum to form zygote in fallopian tube.
    • Zygote develops into blastocyst.
    • Blastocyst implants in endometrium.
    • Upper layer is called epiblast, lower layer is called hypoblast.

    Oogenesis

    • Process of ovum production.
    • Oocytes begin maturing before birth.
    • PGCs differentiate into oogonia.
    • After reaching the gonad, oogonia undergo mitotic divisions.
    • Majority of oogonia in prophase of meiosis I until puberty.
    • Most oogonia degenerate but some survive.
    • Primary oocytes remain arrested until puberty.

    Ovulation

    • Monthly cycles at puberty.
    • Controlled by GnRH from the hypothalamus.
    • Secretion of FSH and LH stimulates ovarian cycle.
    • Several primary follicles stimulated to grow under influence of FSH.

    Zygote Formation

    • Definitive oocyte contains female pronucleus with 23 chromosomes.
    • Sperm tail detaches, its nucleus enters oocyte as male pronucleus.
    • Both pronuclei unite, restoring diploid number.

    Cleavage and Blastocyst Formation

    • Zygote divides to form two cells, then morula (16 cells).
    • Morula differentiates into outer and inner cell masses.
    • Blastocyst forms a cavity within the morula.
    • Blastocyst enters the uterine cavity.
    • Blastocyst digests zona pellucida (hatching).

    Blastocyst Formation

    • Embryonic pole—side of blastocyst to which inner cell mass is attached.
    • Opposite side—abembryonic pole.

    At the End of the 1st Week

    • Embryo has inner cell mass (embryoblast) and outer cell mass (trophoblast).
    • Embryoblast forms embryo proper.
    • Trophoblast contributes to placenta formation.

    Further Development of Embryoblast

    • Epiblast cells adjacent to cytotrophoblast become amnioblasts.
    • Hypoblast forms exocoelomic membrane.
    • Blastocyst cavity becomes exocoelomic cavity or primary yolk sac.
    • Epiblast and hypoblast form bilaminar disc.

    Week 2 Summary

    • Key structures and processes of week 2 development.
    • Important concepts like deep implantation, hCG synthesis, formation of chorionic villi, and the development of the embryo proper.

    Human Chorionic Gonadotropin (hCG)

    • Glycoprotein produced by syncytiotrophoblast.
    • Stimulates progesterone production by corpus luteum.
    • Basis for pregnancy testing.
    • Low hCG level can indicate potential spontaneous abortion or ectopic pregnancy.
    • High hCG level can indicate multiple pregnancy, hydatidiform mole, or gestational trophoblastic neoplasia.

    Gestational Trophoblastic Disease

    • Hydatidiform mole—benign, enlarged chorionic villi, grape-like vesicles.
    • Choriocarcinoma—malignant tumor of trophoblast, often following pregnancy.

    Oncofetal Antigens

    • Cell surface proteins present during fetal development.
    • Can re-express in malignant cells.
    • Used as tumor markers for diagnosis and prognosis (e.g., alpha-fetoprotein, carcinoembryonic antigen).

    Gastrulation, Formation of the Trilaminar Embryonic Disc

    • Conversion of bilaminar disc to a trilaminar embryonic disc (ectoderm, mesoderm, and endoderm).
    • Formation of primitive streak initiates gastrulation.
    • Epiblast cells differentiate into endoderm and mesoderm.

    Formation of Primitive Streak

    • Thickened epiblast band migrating inwards.
    • FGF8 controls cell migration, invagination, and specification.
    • Primitive node forms at the cranial end of the streak.
    • Primitive streak becomes primitive groove and pit, signaling the start of gastrulation.

    Functions of the Primitive Streak

    • Determines the site of gastrulation.
    • Initiates the formation of germ layers.
    • Defines major body axes.
    • Primitive streak fades away by the 4th week.

    Tumors Associated with Gastrulation

    • Sacrococcygeal teratomas: arise from remnants of the primitive streak, containing tissues from all three germ layers.

    Teratogenesis Associated with Gastrulation

    • Third week is a highly sensitive period for teratogenic insults.
    • Teratogens can damage developing organ systems.
    • Examples include alcohol (affecting forebrain and eyes) and leading to holoprosencephaly.

    Neural Tube Formation

    • Neural plate develops into neural tube.
    • Neural folds approach, fuse, and form neural tube.
    • Closure occurs in the cervical region, then cranially and caudally.
    • Cranial and caudal neuropores close at specific times.

    Neural Tube Defects

    • Defects may occur if closure of neural tube fails.
    • Anencephaly—failure to close in the cranial region.
    • Spina bifida—closure failure in the cervical region or below.

    Placenta

    • Temporary organ during pregnancy.
    • Attaches to uterine wall, provides nutrients and oxygen to baby.
    • Two parts: maternal part (decidua basalis) and fetal part (chorion frondosum).

    Maternal Part of Placenta - Decidua

    • Uterine endometrium after implantation.
    • Three types—decidua basalis, capsularis, and parietalis.

    Fetal Part of Placenta - Chorion Frondosum

    • Functional unit is chorionic villi.
    • Villi grow from trophoblast surface.
    • Villi in contact with decidua basalis develop into chorion frondosum.
    • Villi in contact with decidua capsularis degenerate to chorion laeve.

    Twinning

    • Dizygotic (fraternal):
      • Fertilization of two different oocytes by different sperms.
      • Most common type of twins(90%)
      • Each has its own placenta, amnion, and chorion.
      • Genetics different.
      • Incidence increases with maternal age.
    • Monozygotic (identical):
      • Result from embryonic zygote splitting.
      • 10% of twin cases
      • Identical genetics
    • Differentiation times for developing splits dictate the formation of chorion and placenta in monozygotic twins. Different times result in different placentation structures.

    Vanishing Twins

    • One twin dies early during pregnancy and is resorbed or mummified (fetus papyraceus).
    • Twin transfusion syndrome is one of the possible contributing factors.

    Twin Transfusion Syndrome

    • Usually in monozygotic twins sharing a placenta.
    • Abnormal vascular connections, one twin receives more blood than the other.
    • One twin may receive too little blood.

    Striated Skeletal Muscle

    • Cells from VLL region migrate to lateral plate mesoderm to form infrahyoid(neck), abdominal, and limb muscles.
    • Remaining myotome cells form back, shoulder, and intercostal muscles.

    Limb Defects

    • Amelia - Complete absence of one or more limbs.
    • Meromelia - Partial absence of one or more limbs.
    • Micromelia - Abnormally short limbs.
    • Brachydactyly - Shortened digits.
    • Syndactyly - Fused digits.
    • Cleft hand and foot - Abnormal cleft between bones in the hand or foot.
    • Arthrogryposis- Congenital joint contractures.

    Limb Defects - Amniotic Bands

    • Can constrict limbs or digits in the womb.
    • Not precisely understood how these bands develop.

    Preeclampsia

    • Maternal hypertension and proteinuria during pregnancy.
    • Usually during second trimester(20 week mark)
    • Can result in fetal growth retardation or death; leading to maternal complications.
    • Can worsen to eclampsia, characterized by seizures.

    Polydactyly

    • Condition where babies have extra fingers.
    • Caused by duplication of zone of polarizing activity (ZPA).

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