Podcast
Questions and Answers
What is the primary purpose of an ELISA assay?
What is the primary purpose of an ELISA assay?
- To measure the concentration of a specific protein or antigen (correct)
- To analyze the pharmacodynamics of a drug
- To determine the toxicity levels of a drug
- To test the stability of a drug over time
Which of the following is NOT a type of ELISA mentioned in the text?
Which of the following is NOT a type of ELISA mentioned in the text?
- Indirect ELISA
- Sandwich ELISA (correct)
- Competitive ELISA
- Direct ELISA
What is the primary function of the enzyme in an ELISA assay?
What is the primary function of the enzyme in an ELISA assay?
- To provide a colorimetric reaction for visualization purposes (correct)
- To amplify the concentration of the antigen being measured
- To catalyze the binding of antibodies to antigens
- To increase the specificity of the antibody-antigen interaction
Which of the following is NOT a type of assay mentioned in the text?
Which of the following is NOT a type of assay mentioned in the text?
What is the primary difference between a direct and an indirect ELISA?
What is the primary difference between a direct and an indirect ELISA?
What is the purpose of an Investigational New Drug (IND) application?
What is the purpose of an Investigational New Drug (IND) application?
Which technique is used to separate proteins based on their size?
Which technique is used to separate proteins based on their size?
What is the primary purpose of a stability assay?
What is the primary purpose of a stability assay?
Which technique separates molecules based on their overall charge at a given pH?
Which technique separates molecules based on their overall charge at a given pH?
What is the purpose of a pharmacodynamic (PD) assay?
What is the purpose of a pharmacodynamic (PD) assay?
Which technique is used to separate proteins based on their shape or attraction to certain types of chromatography resin?
Which technique is used to separate proteins based on their shape or attraction to certain types of chromatography resin?
Which technique is used to separate molecules based on their hydrophobicity (aversion to water)?
Which technique is used to separate molecules based on their hydrophobicity (aversion to water)?
What is the purpose of a potency assay?
What is the purpose of a potency assay?
What is the primary difference between cation exchange and anion exchange chromatography?
What is the primary difference between cation exchange and anion exchange chromatography?
What is the primary purpose of an activity assay?
What is the primary purpose of an activity assay?
What is the purpose of an Investigational New Drug (IND) application?
What is the purpose of an Investigational New Drug (IND) application?
