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ELISA Molecular Assay Quiz

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Questions and Answers

What is the primary purpose of an ELISA assay?

To measure the concentration of a specific protein or antigen (correct)

To analyze the pharmacodynamics of a drug

To determine the toxicity levels of a drug

To test the stability of a drug over time

Which of the following is NOT a type of ELISA mentioned in the text?

Indirect ELISA

Sandwich ELISA (correct)

Competitive ELISA

Direct ELISA

What is the primary function of the enzyme in an ELISA assay?

To provide a colorimetric reaction for visualization purposes (correct)

To amplify the concentration of the antigen being measured

To catalyze the binding of antibodies to antigens

To increase the specificity of the antibody-antigen interaction

Which of the following is NOT a type of assay mentioned in the text?

Binding assay Signup and view all the answers

What is the primary difference between a direct and an indirect ELISA?

A direct ELISA uses a primary antibody linked to an enzyme, while an indirect ELISA uses a secondary antibody linked to an enzyme Signup and view all the answers

What is the purpose of an Investigational New Drug (IND) application?

To obtain approval for testing a new drug on humans Signup and view all the answers

Which technique is used to separate proteins based on their size?

Gel-filtration chromatography Signup and view all the answers

What is the primary purpose of a stability assay?

To determine the conditions that affect the shelf life of a drug Signup and view all the answers

Which technique separates molecules based on their overall charge at a given pH?

Ion-exchange chromatography Signup and view all the answers

What is the purpose of a pharmacodynamic (PD) assay?

To show how a drug is metabolized in the body Signup and view all the answers

Which technique is used to separate proteins based on their shape or attraction to certain types of chromatography resin?

Affinity chromatography Signup and view all the answers

Which technique is used to separate molecules based on their hydrophobicity (aversion to water)?

Hydrophobic-interaction chromatography Signup and view all the answers

What is the purpose of a potency assay?

To show the biochemical effect of a drug on the body Signup and view all the answers

What is the primary difference between cation exchange and anion exchange chromatography?

Cation exchange binds positively charged ions, while anion exchange binds negatively charged ions Signup and view all the answers

What is the primary purpose of an activity assay?

To show a molecule is conducting the expected reaction Signup and view all the answers

What is the purpose of an Investigational New Drug (IND) application?

To allow testing of a new drug or product on humans Signup and view all the answers

ELISA Molecular Assay Quiz

Choose a study mode

Podcast

Questions and Answers

What is the primary purpose of an ELISA assay?

Which of the following is NOT a type of ELISA mentioned in the text?

What is the primary function of the enzyme in an ELISA assay?

Which of the following is NOT a type of assay mentioned in the text?

What is the primary difference between a direct and an indirect ELISA?

What is the purpose of an Investigational New Drug (IND) application?

Which technique is used to separate proteins based on their size?

What is the primary purpose of a stability assay?

Which technique separates molecules based on their overall charge at a given pH?

What is the purpose of a pharmacodynamic (PD) assay?

Which technique is used to separate proteins based on their shape or attraction to certain types of chromatography resin?

Which technique is used to separate molecules based on their hydrophobicity (aversion to water)?

What is the purpose of a potency assay?

What is the primary difference between cation exchange and anion exchange chromatography?

What is the primary purpose of an activity assay?

What is the purpose of an Investigational New Drug (IND) application?

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