16 Questions
What is the primary purpose of an ELISA assay?
To measure the concentration of a specific protein or antigen
Which of the following is NOT a type of ELISA mentioned in the text?
Sandwich ELISA
What is the primary function of the enzyme in an ELISA assay?
To provide a colorimetric reaction for visualization purposes
Which of the following is NOT a type of assay mentioned in the text?
Binding assay
What is the primary difference between a direct and an indirect ELISA?
A direct ELISA uses a primary antibody linked to an enzyme, while an indirect ELISA uses a secondary antibody linked to an enzyme
What is the purpose of an Investigational New Drug (IND) application?
To obtain approval for testing a new drug on humans
Which technique is used to separate proteins based on their size?
Gel-filtration chromatography
What is the primary purpose of a stability assay?
To determine the conditions that affect the shelf life of a drug
Which technique separates molecules based on their overall charge at a given pH?
Ion-exchange chromatography
What is the purpose of a pharmacodynamic (PD) assay?
To show how a drug is metabolized in the body
Which technique is used to separate proteins based on their shape or attraction to certain types of chromatography resin?
Affinity chromatography
Which technique is used to separate molecules based on their hydrophobicity (aversion to water)?
Hydrophobic-interaction chromatography
What is the purpose of a potency assay?
To show the biochemical effect of a drug on the body
What is the primary difference between cation exchange and anion exchange chromatography?
Cation exchange binds positively charged ions, while anion exchange binds negatively charged ions
What is the primary purpose of an activity assay?
To show a molecule is conducting the expected reaction
What is the purpose of an Investigational New Drug (IND) application?
To allow testing of a new drug or product on humans
Test your knowledge about the Enzyme-Linked Immunosorbent Assay (ELISA), an important molecular assay used for detecting specific proteins. Learn about the principles of ELISA, including antibody antigen specificity and enzyme activity for visualization. This quiz covers topics related to recognizing antigens and measuring their concentrations.
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