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Questions and Answers
What is the principle of SDS-PAGE (SDS-polyacrylamide gel electrophoresis)?
What is the principle of SDS-PAGE (SDS-polyacrylamide gel electrophoresis)?
What does the electrophoretic mobility of a protein depend on?
What does the electrophoretic mobility of a protein depend on?
What factor primarily determines the speed of protein movement in electrophoresis?
What factor primarily determines the speed of protein movement in electrophoresis?
The electrophoretic mobility of a protein is influenced by its shape, charge, and size
The electrophoretic mobility of a protein is influenced by its shape, charge, and size
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What treatment renders all proteins negatively charged in SDS-PAGE?
What treatment renders all proteins negatively charged in SDS-PAGE?
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Electrophoresis separates mixtures of amino acids or fragments of DNA based on charge rather than molecular weight
Electrophoresis separates mixtures of amino acids or fragments of DNA based on charge rather than molecular weight
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In SDS-PAGE, why do smaller polypeptides move faster than larger ones?
In SDS-PAGE, why do smaller polypeptides move faster than larger ones?
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What is the function of ethidium bromide in agarose gel electrophoresis?
What is the function of ethidium bromide in agarose gel electrophoresis?
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In SDS-PAGE, smaller polypeptides move faster because they are positively charged
In SDS-PAGE, smaller polypeptides move faster because they are positively charged
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The principle of SDS-PAGE is to separate proteins based on their molecular weight
The principle of SDS-PAGE is to separate proteins based on their molecular weight
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Electrophoretic mobility of a protein depends on its molecular weight and charge, but not its shape
Electrophoretic mobility of a protein depends on its molecular weight and charge, but not its shape
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What determines the mobility of nucleic acids in agarose gels?
What determines the mobility of nucleic acids in agarose gels?
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Which DNA conformations are most frequently encountered in agarose gel electrophoresis?
Which DNA conformations are most frequently encountered in agarose gel electrophoresis?
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What is the support medium used in agarose gel electrophoresis?
What is the support medium used in agarose gel electrophoresis?
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At what temperature is the agarose solution poured between glass plates in agarose gel electrophoresis?
At what temperature is the agarose solution poured between glass plates in agarose gel electrophoresis?
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