Electrophoresis Techniques Quiz
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Questions and Answers

What is the principle of SDS-PAGE (SDS-polyacrylamide gel electrophoresis)?

  • Separation based on protein shape
  • Separation based on protein charge
  • Separation based on protein size
  • Separation based on protein molecular weight (correct)
  • What does the electrophoretic mobility of a protein depend on?

  • Molecular weight and charge (correct)
  • Shape and charge
  • Size and shape
  • Charge and size
  • What factor primarily determines the speed of protein movement in electrophoresis?

  • Protein size
  • Protein molecular weight (correct)
  • Protein shape
  • Protein charge
  • The electrophoretic mobility of a protein is influenced by its shape, charge, and size

    <p>True</p> Signup and view all the answers

    What treatment renders all proteins negatively charged in SDS-PAGE?

    <p>Treatment with a reducing agent</p> Signup and view all the answers

    Electrophoresis separates mixtures of amino acids or fragments of DNA based on charge rather than molecular weight

    <p>False</p> Signup and view all the answers

    In SDS-PAGE, why do smaller polypeptides move faster than larger ones?

    <p>They face less hindrance</p> Signup and view all the answers

    What is the function of ethidium bromide in agarose gel electrophoresis?

    <p>It enhances the fluorescence of stacked nucleic acid bases</p> Signup and view all the answers

    In SDS-PAGE, smaller polypeptides move faster because they are positively charged

    <p>False</p> Signup and view all the answers

    The principle of SDS-PAGE is to separate proteins based on their molecular weight

    <p>True</p> Signup and view all the answers

    Electrophoretic mobility of a protein depends on its molecular weight and charge, but not its shape

    <p>False</p> Signup and view all the answers

    What determines the mobility of nucleic acids in agarose gels?

    <p>Agarose concentration and nucleic acid molecular size and conformation</p> Signup and view all the answers

    Which DNA conformations are most frequently encountered in agarose gel electrophoresis?

    <p>Superhelical circular (form I), nicked circular (form II), and linear (form III)</p> Signup and view all the answers

    What is the support medium used in agarose gel electrophoresis?

    <p>Agarose</p> Signup and view all the answers

    At what temperature is the agarose solution poured between glass plates in agarose gel electrophoresis?

    <p>50-60°C</p> Signup and view all the answers

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