Podcast
Questions and Answers
What is the principle of SDS-PAGE (SDS-polyacrylamide gel electrophoresis)?
What is the principle of SDS-PAGE (SDS-polyacrylamide gel electrophoresis)?
- Separation based on protein shape
- Separation based on protein charge
- Separation based on protein size
- Separation based on protein molecular weight (correct)
What does the electrophoretic mobility of a protein depend on?
What does the electrophoretic mobility of a protein depend on?
- Molecular weight and charge (correct)
- Shape and charge
- Size and shape
- Charge and size
What factor primarily determines the speed of protein movement in electrophoresis?
What factor primarily determines the speed of protein movement in electrophoresis?
- Protein size
- Protein molecular weight (correct)
- Protein shape
- Protein charge
The electrophoretic mobility of a protein is influenced by its shape, charge, and size
The electrophoretic mobility of a protein is influenced by its shape, charge, and size
What treatment renders all proteins negatively charged in SDS-PAGE?
What treatment renders all proteins negatively charged in SDS-PAGE?
Electrophoresis separates mixtures of amino acids or fragments of DNA based on charge rather than molecular weight
Electrophoresis separates mixtures of amino acids or fragments of DNA based on charge rather than molecular weight
In SDS-PAGE, why do smaller polypeptides move faster than larger ones?
In SDS-PAGE, why do smaller polypeptides move faster than larger ones?
What is the function of ethidium bromide in agarose gel electrophoresis?
What is the function of ethidium bromide in agarose gel electrophoresis?
In SDS-PAGE, smaller polypeptides move faster because they are positively charged
In SDS-PAGE, smaller polypeptides move faster because they are positively charged
The principle of SDS-PAGE is to separate proteins based on their molecular weight
The principle of SDS-PAGE is to separate proteins based on their molecular weight
Electrophoretic mobility of a protein depends on its molecular weight and charge, but not its shape
Electrophoretic mobility of a protein depends on its molecular weight and charge, but not its shape
What determines the mobility of nucleic acids in agarose gels?
What determines the mobility of nucleic acids in agarose gels?
Which DNA conformations are most frequently encountered in agarose gel electrophoresis?
Which DNA conformations are most frequently encountered in agarose gel electrophoresis?
What is the support medium used in agarose gel electrophoresis?
What is the support medium used in agarose gel electrophoresis?
At what temperature is the agarose solution poured between glass plates in agarose gel electrophoresis?
At what temperature is the agarose solution poured between glass plates in agarose gel electrophoresis?
Flashcards
SDS-PAGE Principle
SDS-PAGE Principle
Separation of proteins based on their molecular weight.
Electrophoretic Mobility
Electrophoretic Mobility
Molecular weight and charge.
Protein Movement Speed
Protein Movement Speed
Protein molecular weight.
Electrophoretic Mobility Factors
Electrophoretic Mobility Factors
Signup and view all the flashcards
Negative Charge in SDS-PAGE
Negative Charge in SDS-PAGE
Signup and view all the flashcards
Polypeptide Movement in SDS-PAGE
Polypeptide Movement in SDS-PAGE
Signup and view all the flashcards
Ethidium Bromide Function
Ethidium Bromide Function
Signup and view all the flashcards
Nucleic Acid Mobility
Nucleic Acid Mobility
Signup and view all the flashcards
DNA Conformations
DNA Conformations
Signup and view all the flashcards
Support Medium
Support Medium
Signup and view all the flashcards
Pouring Temperature
Pouring Temperature
Signup and view all the flashcards
