15 Questions
What is the principle of SDS-PAGE (SDS-polyacrylamide gel electrophoresis)?
Separation based on protein molecular weight
What does the electrophoretic mobility of a protein depend on?
Molecular weight and charge
What factor primarily determines the speed of protein movement in electrophoresis?
Protein molecular weight
The electrophoretic mobility of a protein is influenced by its shape, charge, and size
True
What treatment renders all proteins negatively charged in SDS-PAGE?
Treatment with a reducing agent
Electrophoresis separates mixtures of amino acids or fragments of DNA based on charge rather than molecular weight
False
In SDS-PAGE, why do smaller polypeptides move faster than larger ones?
They face less hindrance
What is the function of ethidium bromide in agarose gel electrophoresis?
It enhances the fluorescence of stacked nucleic acid bases
In SDS-PAGE, smaller polypeptides move faster because they are positively charged
False
The principle of SDS-PAGE is to separate proteins based on their molecular weight
True
Electrophoretic mobility of a protein depends on its molecular weight and charge, but not its shape
False
What determines the mobility of nucleic acids in agarose gels?
Agarose concentration and nucleic acid molecular size and conformation
Which DNA conformations are most frequently encountered in agarose gel electrophoresis?
Superhelical circular (form I), nicked circular (form II), and linear (form III)
What is the support medium used in agarose gel electrophoresis?
Agarose
At what temperature is the agarose solution poured between glass plates in agarose gel electrophoresis?
50-60°C
Test your knowledge of electrophoresis techniques with this quiz. Learn about the principles and processes of electrophoresis, including SDS-PAGE and the separation of amino acids and DNA fragments.
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