Questions and Answers
What is the purpose of using a Corning during the preparation of electrophoresis?
Which of the following is a common use of gel electrophoresis in medical diagnostics?
Which of the following are required for electrophoresis?
What is the light energy absorbed and emitted called?
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What is the purpose of using a comb during the preparation of a gel for electrophoresis?
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Which of the following is a common use of gel electrophoresis in medical diagnostics?
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Which of the following is an example of adsorption chromatography?
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What are the two main components required for all chromatographic methods?
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Which technique is commonly used to compare unknown samples against standards in electrophoresis?
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Which of the following is an essential step to achieve better separation during gel electrophoresis?
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Study Notes
Gel Electrophoresis
- The purpose of using Corning in the preparation of electrophoresis is to polymerize the gel.
- Gel electrophoresis is commonly used in medical diagnostics for DNA sequencing and detecting genetic mutations.
- A comb is used during the preparation of a gel for electrophoresis to create wells for loading samples.
Chromatography
- The two main components required for all chromatographic methods are the stationary phase and mobile phase.
- An example of adsorption chromatography is chromatography using a column containing silica gel or alumina.
- The components required for electrophoresis are not adsorbent and mobile phase, but rather stationary phase and mobile phase.
Spectrophotometry
- The light energy absorbed and emitted is called transmittance.
Electrophoresis Techniques
- The voltage can be increased to improve separation in electrophoresis.
- Using a different type of gel or comparing with known standards can also improve separation in electrophoresis.
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Description
Test your understanding of electrophoresis concepts, including preparation, applications, and components. Assess your knowledge of this laboratory technique used in molecular biology.
