Electrophoresis Concepts
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Questions and Answers

What is the purpose of using a Corning during the preparation of electrophoresis?

  • To maintain pH balance
  • To fix the gel
  • To remove water
  • To polymerize the gel (correct)
  • Which of the following is a common use of gel electrophoresis in medical diagnostics?

  • Polymerase chain reaction
  • Chromatography
  • Cloning
  • DNA sequencing (correct)
  • Which of the following are required for electrophoresis?

  • Electric field and gel matrix (correct)
  • Stationary phase and mobile phase
  • Adsorbent and mobile phase
  • Buffer solution and chromatography paper
  • What is the light energy absorbed and emitted called?

    <p>Transmittance</p> Signup and view all the answers

    What is the purpose of using a comb during the preparation of a gel for electrophoresis?

    <p>To create wells for loading samples</p> Signup and view all the answers

    Which of the following is a common use of gel electrophoresis in medical diagnostics?

    <p>Detecting genetic mutations</p> Signup and view all the answers

    Which of the following is an example of adsorption chromatography?

    <p>Chromatography using a column containing silica gel or alumina</p> Signup and view all the answers

    What are the two main components required for all chromatographic methods?

    <p>Stationary phase and mobile phase</p> Signup and view all the answers

    Which technique is commonly used to compare unknown samples against standards in electrophoresis?

    <p>By comparing with known standards</p> Signup and view all the answers

    Which of the following is an essential step to achieve better separation during gel electrophoresis?

    <p>Increasing the voltage</p> Signup and view all the answers

    Study Notes

    Gel Electrophoresis

    • The purpose of using Corning in the preparation of electrophoresis is to polymerize the gel.
    • Gel electrophoresis is commonly used in medical diagnostics for DNA sequencing and detecting genetic mutations.
    • A comb is used during the preparation of a gel for electrophoresis to create wells for loading samples.

    Chromatography

    • The two main components required for all chromatographic methods are the stationary phase and mobile phase.
    • An example of adsorption chromatography is chromatography using a column containing silica gel or alumina.
    • The components required for electrophoresis are not adsorbent and mobile phase, but rather stationary phase and mobile phase.

    Spectrophotometry

    • The light energy absorbed and emitted is called transmittance.

    Electrophoresis Techniques

    • The voltage can be increased to improve separation in electrophoresis.
    • Using a different type of gel or comparing with known standards can also improve separation in electrophoresis.

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    Description

    Test your understanding of electrophoresis concepts, including preparation, applications, and components. Assess your knowledge of this laboratory technique used in molecular biology.

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