Podcast
Questions and Answers
What is the purpose of using a Corning during the preparation of electrophoresis?
What is the purpose of using a Corning during the preparation of electrophoresis?
- To maintain pH balance
- To fix the gel
- To remove water
- To polymerize the gel (correct)
Which of the following is a common use of gel electrophoresis in medical diagnostics?
Which of the following is a common use of gel electrophoresis in medical diagnostics?
- Polymerase chain reaction
- Chromatography
- Cloning
- DNA sequencing (correct)
Which of the following are required for electrophoresis?
Which of the following are required for electrophoresis?
- Electric field and gel matrix (correct)
- Stationary phase and mobile phase
- Adsorbent and mobile phase
- Buffer solution and chromatography paper
What is the light energy absorbed and emitted called?
What is the light energy absorbed and emitted called?
What is the purpose of using a comb during the preparation of a gel for electrophoresis?
What is the purpose of using a comb during the preparation of a gel for electrophoresis?
Which of the following is a common use of gel electrophoresis in medical diagnostics?
Which of the following is a common use of gel electrophoresis in medical diagnostics?
Which of the following is an example of adsorption chromatography?
Which of the following is an example of adsorption chromatography?
What are the two main components required for all chromatographic methods?
What are the two main components required for all chromatographic methods?
Which technique is commonly used to compare unknown samples against standards in electrophoresis?
Which technique is commonly used to compare unknown samples against standards in electrophoresis?
Which of the following is an essential step to achieve better separation during gel electrophoresis?
Which of the following is an essential step to achieve better separation during gel electrophoresis?
Study Notes
Gel Electrophoresis
- The purpose of using Corning in the preparation of electrophoresis is to polymerize the gel.
- Gel electrophoresis is commonly used in medical diagnostics for DNA sequencing and detecting genetic mutations.
- A comb is used during the preparation of a gel for electrophoresis to create wells for loading samples.
Chromatography
- The two main components required for all chromatographic methods are the stationary phase and mobile phase.
- An example of adsorption chromatography is chromatography using a column containing silica gel or alumina.
- The components required for electrophoresis are not adsorbent and mobile phase, but rather stationary phase and mobile phase.
Spectrophotometry
- The light energy absorbed and emitted is called transmittance.
Electrophoresis Techniques
- The voltage can be increased to improve separation in electrophoresis.
- Using a different type of gel or comparing with known standards can also improve separation in electrophoresis.
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Description
Test your understanding of electrophoresis concepts, including preparation, applications, and components. Assess your knowledge of this laboratory technique used in molecular biology.
