Podcast
Questions and Answers
What is the primary advantage of using a confocal pinhole in laser scanning confocal microscopy?
What is the primary advantage of using a confocal pinhole in laser scanning confocal microscopy?
- To ensure that the laser only excites one spot at a time
- To eliminate out-of-focus fluorescence coming from the sample (correct)
- To prevent damage to the objective lenses due to the high-intensity light of the laser
- To increase the energy of excitation and decrease the limit of resolution
What is the relationship between the emission wavelength and excitation wavelength of a fluorophore?
What is the relationship between the emission wavelength and excitation wavelength of a fluorophore?
- The emission wavelength is always shorter than the excitation wavelength
- The emission wavelength is always longer than the excitation wavelength (correct)
- The emission wavelength is always equal to the excitation wavelength
- The emission wavelength is irrelevant to the excitation wavelength
Which of the following fluorophores would provide the highest quality images in fluorescence microscopy?
Which of the following fluorophores would provide the highest quality images in fluorescence microscopy?
- Wavelength is irrelevant to the quality of images
- Hoecsht 33342 with an emission wavelength of 461 nm (correct)
- eGFP with an emission wavelength of 509 nm
- Flutax with an emission wavelength of 520 nm
What is the purpose of the pinhole aperture in laser scanning confocal microscopy?
What is the purpose of the pinhole aperture in laser scanning confocal microscopy?
What is the effect of the confocal pinhole on the resolution of images in laser scanning confocal microscopy?
What is the effect of the confocal pinhole on the resolution of images in laser scanning confocal microscopy?
Why is eGFP often used as a fluorescent tag in cells?
Why is eGFP often used as a fluorescent tag in cells?
What is the relationship between the emission energy and excitation energy of a fluorophore?
What is the relationship between the emission energy and excitation energy of a fluorophore?
What is the primary advantage of using fluorescence microscopy over other forms of microscopy?
What is the primary advantage of using fluorescence microscopy over other forms of microscopy?
What is the purpose of the objective lens in laser scanning confocal microscopy?
What is the purpose of the objective lens in laser scanning confocal microscopy?
What is the effect of the emission wavelength of a fluorophore on the quality of images in fluorescence microscopy?
What is the effect of the emission wavelength of a fluorophore on the quality of images in fluorescence microscopy?
