Electron Transport and Spectrophotometry in Bacteria
10 Questions
0 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the primary advantage of using a confocal pinhole in laser scanning confocal microscopy?

  • To ensure that the laser only excites one spot at a time
  • To eliminate out-of-focus fluorescence coming from the sample (correct)
  • To prevent damage to the objective lenses due to the high-intensity light of the laser
  • To increase the energy of excitation and decrease the limit of resolution

    • What is the relationship between the emission wavelength and excitation wavelength of a fluorophore?

  • The emission wavelength is always shorter than the excitation wavelength
  • The emission wavelength is always longer than the excitation wavelength (correct)
  • The emission wavelength is always equal to the excitation wavelength
  • The emission wavelength is irrelevant to the excitation wavelength

    • Which of the following fluorophores would provide the highest quality images in fluorescence microscopy?

  • Wavelength is irrelevant to the quality of images
  • Hoecsht 33342 with an emission wavelength of 461 nm (correct)
  • eGFP with an emission wavelength of 509 nm
  • Flutax with an emission wavelength of 520 nm

    • What is the purpose of the pinhole aperture in laser scanning confocal microscopy?

    <p>To eliminate out-of-focus fluorescence coming from the sample</p> Signup and view all the answers

    What is the effect of the confocal pinhole on the resolution of images in laser scanning confocal microscopy?

    <p>It decreases the limit of resolution</p> Signup and view all the answers

    Why is eGFP often used as a fluorescent tag in cells?

    <p>Because it can be easily expressed in cells</p> Signup and view all the answers

    What is the relationship between the emission energy and excitation energy of a fluorophore?

    <p>The emission energy is always lower than the excitation energy</p> Signup and view all the answers

    What is the primary advantage of using fluorescence microscopy over other forms of microscopy?

    <p>It allows for specific labeling of structures or proteins</p> Signup and view all the answers

    What is the purpose of the objective lens in laser scanning confocal microscopy?

    <p>To collect the fluorescence signal from the sample</p> Signup and view all the answers

    What is the effect of the emission wavelength of a fluorophore on the quality of images in fluorescence microscopy?

    <p>It increases the resolution of images</p> Signup and view all the answers

    More Like This

    Use Quizgecko on...
    Browser
    Open
    Browser
    Browser