10 Questions
What type of microscopy uses a beam of electrons to produce an image of the sample?
Electron Microscopy
Which type of electron microscopy involves scanning the surface of the sample with electrons?
Scanning Electron Microscopy (SEM)
What is the main difference between Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM)?
TEM passes electrons through the sample, while SEM scans the surface
Which type of microscopy is commonly used for 3D imaging and optical sectioning?
Confocal Microscopy
What is the function of the plasma membrane in a cell?
To regulate what enters and leaves the cell
What is the main function of the nucleus in a cell?
To contain genetic material
Which type of microscopy has a higher resolution and magnification than light microscopy?
All of the above
What is the main difference between Brightfield microscopy and Darkfield microscopy?
Brightfield shows the sample as bright, while Darkfield shows the sample as dark
Which type of microscopy uses a physical probe to scan the surface of the sample?
Scanning Probe Microscopy
What is the function of organelles in a cell?
To perform specialized functions
Study Notes
Electron Microscopy
- Uses a beam of electrons to produce an image of the sample
- Higher resolution and magnification than light microscopy
- Sample preparation involves fixation, dehydration, and staining with heavy metals
- Two main types:
- Transmission Electron Microscopy (TEM): electrons pass through the sample
- Scanning Electron Microscopy (SEM): electrons scan the surface of the sample
Microscope Types
-
Light Microscopy:
- Uses visible light to produce an image
- Limited resolution and magnification (up to 1000x)
- Types:
- Brightfield microscopy
- Darkfield microscopy
- Fluorescence microscopy
- Phase contrast microscopy
-
Confocal Microscopy:
- Uses laser light to produce a high-resolution image
- Higher resolution and magnification than light microscopy
- Allows for 3D imaging and optical sectioning
-
Scanning Probe Microscopy:
- Uses a physical probe to scan the surface of the sample
- High resolution and magnification (up to 100,000x)
- Types:
- Atomic Force Microscopy (AFM)
- Scanning Tunneling Microscopy (STM)
Cell Biology
-
Cell Structure:
- Plasma membrane: semi-permeable membrane surrounding the cell
- Cytoplasm: jelly-like substance inside the cell
- Nucleus: contains genetic material
- Organelles: specialized structures within the cell (e.g. mitochondria, ribosomes)
-
Cellular Processes:
- Cell division: mitosis and meiosis
- Cell signaling: communication between cells
- Cell adhesion: cells sticking together
- Cell migration: cells moving from one place to another
-
Cellular Components:
- Cytoskeleton: network of filaments providing structural support
- Vesicles: membrane-bound sacs for transporting materials
- Lysosomes: contain digestive enzymes for breaking down waste
- Peroxisomes: involved in cellular respiration and detoxification
Electron Microscopy
- Uses a beam of electrons to produce an image of the sample
- Offers higher resolution and magnification than light microscopy
- Sample preparation involves fixation, dehydration, and staining with heavy metals
- Two main types:
- Transmission Electron Microscopy (TEM): electrons pass through the sample
- Scanning Electron Microscopy (SEM): electrons scan the surface of the sample
Microscope Types
- Light Microscopy
- Uses visible light to produce an image
- Limited resolution and magnification (up to 1000x)
- Types:
- Brightfield microscopy
- Darkfield microscopy
- Fluorescence microscopy
- Phase contrast microscopy
- Confocal Microscopy
- Uses laser light to produce a high-resolution image
- Higher resolution and magnification than light microscopy
- Allows for 3D imaging and optical sectioning
- Scanning Probe Microscopy
- Uses a physical probe to scan the surface of the sample
- High resolution and magnification (up to 100,000x)
- Types:
- Atomic Force Microscopy (AFM)
- Scanning Tunneling Microscopy (STM)
Cell Biology
- Cell Structure
- Plasma membrane: semi-permeable membrane surrounding the cell
- Cytoplasm: jelly-like substance inside the cell
- Nucleus: contains genetic material
- Organelles: specialized structures within the cell (e.g.mitochondria, ribosomes)
- Cellular Processes
- Cell division: mitosis and meiosis
- Cell signaling: communication between cells
- Cell adhesion: cells sticking together
- Cell migration: cells moving from one place to another
- Cellular Components
- Cytoskeleton: network of filaments providing structural support
- Vesicles: membrane-bound sacs for transporting materials
- Lysosomes: contain digestive enzymes for breaking down waste
- Peroxisomes: involved in cellular respiration and detoxification
Understand the principles and types of electron microscopy, including transmission and scanning electron microscopy, and compare it with light microscopy.
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