Effects of κ-Casein Splitting on Milk Curdling
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Questions and Answers

What effect does the acid-rich κ-casein end have on the α-casein and β-casein within the micelles?

  • It enhances their solubility in water.
  • It reduces the negative charges in the micelles.
  • It promotes the disintegration of micelles.
  • It protects them from precipitation by milk calcium. (correct)

What keeps the integrity of the casein micelles?

  • Magnesium sulfate and acid-base interactions between sub-micelles.
  • Sodium chloride and hydrophilic interactions between sub-micelles.
  • Calcium phosphate and hydrophobic interactions between sub-micelles. (correct)
  • Potassium nitrate and covalent bonds between sub-micelles.

At what pH do the micelles repel each other due to a surplus of negative charges?

  • 6.6 (correct)
  • 8.0
  • 7.5
  • 4.0

What happens to micelles if Ca++ leaves them?

<p>Micelles disintegrate into submicelles. (C)</p> Signup and view all the answers

How is water involved in maintaining the balance within micelles?

<p>Water held by κ-casein helps to keep a balance of negative charges between micelles. (B)</p> Signup and view all the answers

What role does rennin play in the coagulation process of casein micelles?

<p>Rennin aids in the aggregation of casein micelles. (D)</p> Signup and view all the answers

What causes curt (coagulated protein) formation in κ-casein when the hydrophilic CMP tail is removed?

<p>Loss of hydrophilic character and disturbance in balance (A)</p> Signup and view all the answers

What happens to the stability of micellar complex when the pH of milk is lowered?

<p>It is disrupted (A)</p> Signup and view all the answers

At which pH does casein start to form a gel due to the same number of positive and negative charges on the casein molecule?

<p>4.6 (D)</p> Signup and view all the answers

Why do sub-micelles and micelles repel each other at pH 6.6?

<p>The protein molecule has a slightly negative charge (C)</p> Signup and view all the answers

What effect does rennin have on casein micelles?

<p>Destabilizes them (A)</p> Signup and view all the answers

What happens to casein molecules at pH 4.6?

<p>They have an equal number of positive and negative charges (B)</p> Signup and view all the answers

What is the function of rennin in cheese making?

<p>To attack the bond between AA 105 and 106 in κ-casein (C)</p> Signup and view all the answers

What is the outcome of splitting the phenylalanine-methionine peptide bond by rennin?

<p>Formation of a tough and rubbery gel (A)</p> Signup and view all the answers

What occurs during the coagulation process of milk micelles?

<p>Formation of a web by para-κ-caseins with calcium and phosphate (A)</p> Signup and view all the answers

What effect does acidification have on casein in dairy products like yogurt?

<p>Serves as the only thickener without using rennet (B)</p> Signup and view all the answers

What leads to the formation of a tough and rubbery gel during cheese making?

<p>Development of calcium bonds when water leaves the structure (A)</p> Signup and view all the answers

What is significant about the iso-electric point (IEP) for casein molecules?

<p>It is where casein molecules carry no charge resulting in precipitation (B)</p> Signup and view all the answers

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