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Questions and Answers

What role do sigma factors play in transcription?

  • They help RNA polymerase recognize specific promoter sequences. (correct)
  • They inhibit the binding of RNA polymerase to the promoter.
  • They degrade mRNA to facilitate transcription.
  • They enhance DNA replication speed.

What is the wobble hypothesis primarily concerned with?

  • The role of ribosomal RNA in translation.
  • The complete fidelity of tRNA codon pairing.
  • The flexibility of the third position of a codon allowing tRNA to recognize multiple codons. (correct)
  • The process of DNA replication.

Which option best describes an inducer in gene expression?

  • It is a signal that turns off gene expression.
  • It is something that continuously activates a gene.
  • It permanently alters the gene's coding sequence.
  • It is a specific substrate that induces gene expression. (correct)

Which event occurs first in initiating translation in prokaryotes?

<p>The mRNA binds to the small ribosomal subunit. (C)</p> Signup and view all the answers

How do type III secretion systems function in Gram-negative bacteria?

<p>They inject proteins directly into host cells. (C)</p> Signup and view all the answers

What describes the role of small RNAs (sRNAs) in bacterial translation?

<p>They block translation by binding to the ribosome binding site of mRNA. (B)</p> Signup and view all the answers

In negative transcriptional control, what is the role of a repressor protein?

<p>It inhibits transcription by binding to the operator. (B)</p> Signup and view all the answers

What mechanism allows transcription and translation to occur simultaneously in bacteria?

<p>The lack of nuclear membranes in prokaryotes. (A)</p> Signup and view all the answers

Why is the term 'prokaryotic' considered outdated?

<p>It does not account for differences between Bacteria and Archaea. (D)</p> Signup and view all the answers

Which of the following is NOT a characteristic of bacterial cells when compared to plant or animal cells?

<p>Bacterial cells contain membrane-bound organelles like mitochondria. (D)</p> Signup and view all the answers

What is a distinctive feature of gram-positive bacteria compared to gram-negative bacteria?

<p>Gram-positive bacteria have thick peptidoglycan layers. (A)</p> Signup and view all the answers

Which transport mechanism chemically modifies the molecule being transported?

<p>Group translocation (C)</p> Signup and view all the answers

What structure serves as a storage compartment for nutrients in bacterial cells?

<p>Inclusions (B)</p> Signup and view all the answers

How does the archaeal cell envelope primarily differ from bacterial cell walls?

<p>It is often made of protein or glycoprotein. (D)</p> Signup and view all the answers

What describes the function of the prokaryotic plasma membrane?

<p>It serves as a selectively permeable barrier for nutrient transport. (D)</p> Signup and view all the answers

What is a key feature of endospores in bacteria?

<p>They are highly resistant structures for survival under harsh conditions. (C)</p> Signup and view all the answers

What is the role of polyribosomes in bacteria?

<p>They enable multiple ribosomes to translate a single mRNA simultaneously. (A)</p> Signup and view all the answers

Which statement accurately matches the secretion systems in bacteria?

<p>Secretion moves proteins from the cytoplasm to external environments. (A), Secretion moves proteins across or into the plasma membrane. (B)</p> Signup and view all the answers

Which type of microbe is characterized by having chitin in its cell walls, forming multicellular filaments, and producing spores?

<p>Fungus (A)</p> Signup and view all the answers

Which feature is unique to bacterial cells compared to plant and animal cells?

<p>Presence of a nucleoid (A)</p> Signup and view all the answers

What determines the unique function of the Sec and Tat systems in bacterial protein secretion?

<p>Sec moves unfolded proteins, while Tat transports folded proteins. (D)</p> Signup and view all the answers

Which characteristic was originally used to classify prokaryotic cells?

<p>The lack of membrane-bound organelles. (D)</p> Signup and view all the answers

Which secretion system is crucial for the pathogenicity of bacteria by exporting effector proteins?

<p>Type III secretion system (A)</p> Signup and view all the answers

What is a key difference between gram-positive and gram-negative bacterial cell walls?

<p>Gram-positive bacteria have a thin peptidoglycan layer. (D)</p> Signup and view all the answers

Flashcards

Polyribosome in bacteria

A complex of multiple ribosomes translating a single mRNA molecule simultaneously; enables rapid protein synthesis.

Translocation

The process of moving proteins across or into the plasma membrane.

Secretion

The process of moving proteins from the cytoplasm to external environments.

Sec and Tat systems

Systems common to both Gram-positive and Gram-negative bacteria, responsible for protein secretion and translocation.

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Effector proteins

Bacterial proteins contributing to the pathogenicity of a bacteria by causing host injury.

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Prokaryotic cells characteristic

Lack a nucleus and membrane-bound organelles; have a nucleoid.

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Gram-positive vs Gram-negative cell walls

Gram-positive walls have thick peptidoglycan layers, while gram-negative walls have thin peptidoglycan layers and an outer membrane.

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Eukaryotic Microorganism example

Organism is a fungus that has chitin in its cell wall, forms filaments and spores.

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Bacterial transformation

A process where bacteria take up foreign DNA from their surroundings

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DNA replication, transcription, and translation

Processes crucial for gene expression and protein synthesis

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Sigma factors

Proteins that facilitate RNA polymerase binding to specific promoter regions in transcription initiation

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Wobble hypothesis

The third position in a codon can tolerate mismatches, enhancing the recognition ability of mRNA and tRNA. The wobble position can accommodate variations.

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Translation initiation complex (bacteria)

mRNA binds to the small ribosomal subunit to initiate protein synthesis

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Attenuation

Transcriptional control mechanism where translation of a leader peptide regulates the transcription of an operon.

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Type III secretion systems

Molecular syringes in Gram-negative bacteria that inject proteins directly into host cells.

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Inducible gene

Gene expressed only when a specific substrate is present; otherwise, it's not expressed.

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Prokaryotic cell outdated?

The term "prokaryotic" is outdated because it doesn't distinguish between Bacteria and Archaea, implying a lack of complexity in bacterial cells.

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Bacterial cell vs. plant/animal cell

Bacterial cells are typically smaller, have a nucleoid instead of a nucleus, lack membrane-bound organelles like mitochondria, and have a variety of shapes (cocci, bacilli, spirilla).

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Gram-positive cell wall

Gram-positive bacteria have a thick peptidoglycan layer, are purple after staining, have a smaller periplasmic space, are more porous and susceptible to antibiotics, and contain teichoic acids in their cell walls.

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Gram-negative cell wall

Gram-negative bacteria have a thin peptidoglycan layer, an outer membrane (LPS), are pink after staining, a larger periplasmic space, less porous, and less susceptible to antibiotics.

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Prokaryotic plasma membrane

A phospholipid bilayer with embedded proteins, serving as a selectively permeable barrier for nutrient transport, waste removal, and energy production (respiration/photosynthesis).

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Group translocation

A transport mechanism that chemically modifies the transported molecule during transport.

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Endospore

A dormant, highly resistant structure for survival under harsh conditions (heat, UV, starvation).

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Archaeal cell envelope

Often consists of a protein or glycoprotein S-layer, without peptidoglycan.

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Study Notes

General Study Notes

  • Use concise and engaging language, avoiding repetitive information.
  • Focus on key facts, figures, and entities.
  • Ensure factual accuracy and originality.
  • Employ markdown formatting.
  • Each point should be independent and self-explanatory.

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