E.coli Lactose Metabolism Proteins Quiz
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Questions and Answers

What molecule represses the production of β-galactosidase in the presence of glucose in lactose metabolism?

  • CAP protein
  • Cyclic AMP
  • Glucose (correct)
  • Lactose

Which protein binds near the lac promoter to trigger transcription in the lac operon?

  • CAP protein (correct)
  • Cyclic AMP
  • β-galactosidase
  • Lactose

What activates expression of the lac operon through positive control?

  • Glucose
  • Lactose
  • Cyclic AMP (correct)
  • β-galactosidase

In the lac operon, which mutation would result in the inability to produce β-galactosidase?

<p>lacZ- mutants (C)</p> Signup and view all the answers

Which molecule regulates the activity of the CAP protein in the lac operon?

<p>Cyclic AMP (D)</p> Signup and view all the answers

What occurs when glucose is abundant in an E. coli cell?

<p>Positive control of the lac operon occurs (C)</p> Signup and view all the answers

What do lacZ- mutants lack?

<p>β-galactosidase (A)</p> Signup and view all the answers

Which protein is lacking in lacY- mutants?

<p>Lactose permease (D)</p> Signup and view all the answers

What is the characteristic of lacI- mutants?

<p>Produce β-galactosidase and galactoside permease even in the absence of lactose (D)</p> Signup and view all the answers

Which two proteins are essential for E. coli to utilize lactose?

<p>Lactose permease and β-galactosidase (B)</p> Signup and view all the answers

What is the regulatory function of the lacI gene product?

<p>Shutting down expression of lacZ and lacY in the presence of lactose (C)</p> Signup and view all the answers

What controls the expression of lacZ and lacY genes in E. coli?

<p>lacI gene product (D)</p> Signup and view all the answers

What is the function of lactose permease in E.coli?

<p>Transporting lactose into the cell (C)</p> Signup and view all the answers

Which type of mutant of E.coli would not be able to metabolize lactose?

<p>All of the above mutants (D)</p> Signup and view all the answers

When both glucose and lactose are present, what happens to β-galactosidase production in E.coli?

<p>It is produced at lower levels (B)</p> Signup and view all the answers

Which mutant strain of E.coli would have a defective β-galactosidase enzyme?

<p>lacZ- mutants (B)</p> Signup and view all the answers

What is the role of lacI gene in E.coli gene regulation?

<p>Repressing the expression of β-galactosidase (C)</p> Signup and view all the answers

What happens to lactose metabolism in lacY- mutants of E.coli?

<p>It stops completely (C)</p> Signup and view all the answers

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