E.coli Lactose Metabolism Proteins Quiz

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What molecule represses the production of β-galactosidase in the presence of glucose in lactose metabolism?

CAP protein

Cyclic AMP

Glucose (correct)

Lactose

Which protein binds near the lac promoter to trigger transcription in the lac operon?

CAP protein (correct)

Cyclic AMP

β-galactosidase

Lactose

What activates expression of the lac operon through positive control?

Glucose

Lactose

Cyclic AMP (correct)

β-galactosidase

In the lac operon, which mutation would result in the inability to produce β-galactosidase?

lacZ- mutants Signup and view all the answers

Which molecule regulates the activity of the CAP protein in the lac operon?

Cyclic AMP Signup and view all the answers

What occurs when glucose is abundant in an E. coli cell?

Positive control of the lac operon occurs Signup and view all the answers

What do lacZ- mutants lack?

β-galactosidase Signup and view all the answers

Which protein is lacking in lacY- mutants?

Lactose permease Signup and view all the answers

What is the characteristic of lacI- mutants?

Produce β-galactosidase and galactoside permease even in the absence of lactose Signup and view all the answers

Which two proteins are essential for E. coli to utilize lactose?

Lactose permease and β-galactosidase Signup and view all the answers

What is the regulatory function of the lacI gene product?

Shutting down expression of lacZ and lacY in the presence of lactose Signup and view all the answers

What controls the expression of lacZ and lacY genes in E. coli?

lacI gene product Signup and view all the answers

What is the function of lactose permease in E.coli?

Transporting lactose into the cell Signup and view all the answers

Which type of mutant of E.coli would not be able to metabolize lactose?

All of the above mutants Signup and view all the answers

When both glucose and lactose are present, what happens to β-galactosidase production in E.coli?

It is produced at lower levels Signup and view all the answers

Which mutant strain of E.coli would have a defective β-galactosidase enzyme?

lacZ- mutants Signup and view all the answers

What is the role of lacI gene in E.coli gene regulation?

Repressing the expression of β-galactosidase Signup and view all the answers

What happens to lactose metabolism in lacY- mutants of E.coli?

It stops completely Signup and view all the answers

E.coli Lactose Metabolism Proteins Quiz

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Questions and Answers

What molecule represses the production of β-galactosidase in the presence of glucose in lactose metabolism?

Which protein binds near the lac promoter to trigger transcription in the lac operon?

What activates expression of the lac operon through positive control?

In the lac operon, which mutation would result in the inability to produce β-galactosidase?

Which molecule regulates the activity of the CAP protein in the lac operon?

What occurs when glucose is abundant in an E. coli cell?

What do lacZ- mutants lack?

Which protein is lacking in lacY- mutants?

What is the characteristic of lacI- mutants?

Which two proteins are essential for E. coli to utilize lactose?

What is the regulatory function of the lacI gene product?

What controls the expression of lacZ and lacY genes in E. coli?

What is the function of lactose permease in E.coli?

Which type of mutant of E.coli would not be able to metabolize lactose?

When both glucose and lactose are present, what happens to β-galactosidase production in E.coli?

Which mutant strain of E.coli would have a defective β-galactosidase enzyme?

What is the role of lacI gene in E.coli gene regulation?

What happens to lactose metabolism in lacY- mutants of E.coli?

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