Early Life Forms and Infectious Diseases

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Questions and Answers

What significant characteristic is presumed to have been present in the earliest forms of life on Earth?

  • Aerobic bacteria
  • Eukaryotic cells
  • Anaerobic bacteria (correct)
  • Multicellular organisms

What event is suggested to have contributed to microbial diversity following the evolution of prokaryotes?

  • The introduction of fermentation processes
  • The development of eukaryotic cells
  • The availability of oxygen due to photosynthesis (correct)
  • The emergence of multicellular organisms

Which historical figure first suggested the idea of animate agents causing disease?

  • Robert Koch
  • Louis Pasteur
  • Aristotle
  • Girolamo Fracastoro (correct)

Which advancement confirmed the existence of microorganisms that could not be seen without magnification?

<p>The invention of the microscope (B)</p> Signup and view all the answers

What traditional belief attributed the causes of diseases to supernatural events?

<p>Miasma theory (C)</p> Signup and view all the answers

How did Pasteur and Koch contribute to the understanding of infectious diseases?

<p>By confirming microbial aetiology of diseases (D)</p> Signup and view all the answers

Which significant change in the field of microbiology occurred in the middle of the nineteenth century?

<p>Confirmation of microorganisms as disease agents (A)</p> Signup and view all the answers

What subjects are significantly impacted by the study of microbiology?

<p>Human health and food preservation (C)</p> Signup and view all the answers

What significant discovery did Bawden and Pirie make regarding tobacco mosaic virus (TMV)?

<p>TMV contains nucleic acid and proteins. (B)</p> Signup and view all the answers

Which of the following criteria was NOT part of Lwoff's universal system for classifying viruses?

<p>The method of replication (C)</p> Signup and view all the answers

What was the role of reverse transcriptase as discovered by Temin and Baltimore?

<p>It helps elucidate retrovirus replication. (B)</p> Signup and view all the answers

What aspect of microbiology is emphasized within veterinary microbiology?

<p>Microorganisms involved in infectious diseases of animals. (A)</p> Signup and view all the answers

Which advancement significantly contributed to basic research on oncogenes and malignant tumours?

<p>Studies on retroviruses (D)</p> Signup and view all the answers

What suggestion did Watson and Crick make regarding viral nucleic acid structure?

<p>It is surrounded by identical protein subunits. (A)</p> Signup and view all the answers

Which of the following statements is correct regarding modern microbiology?

<p>It includes the study of bacteria, fungi, and viruses. (C)</p> Signup and view all the answers

How did the crystallization of TMV by Stanley in 1935 impact virology?

<p>It advanced the understanding of virus chemical composition. (D)</p> Signup and view all the answers

Who recognized the causative organisms of tuberculosis and typhoid fever?

<p>Robert Koch (C)</p> Signup and view all the answers

What is the main purpose of Koch's postulates?

<p>To determine the cause of infectious diseases (B)</p> Signup and view all the answers

Which of the following diseases was first linked to a filterable agent identified by Loeffler and Frosch?

<p>Foot-and-mouth disease (C)</p> Signup and view all the answers

What significant advancement in microbiology occurred in the early 1950s?

<p>Development of continuous cell cultures (D)</p> Signup and view all the answers

Which scientist introduced the smallpox vaccine?

<p>Edward Jenner (A)</p> Signup and view all the answers

Who first reported the filterability of the tobacco mosaic virus?

<p>Dmitri Ivanovsky (B)</p> Signup and view all the answers

Which method was key for recovering large quantities of the vaccinia virus in the early 20th century?

<p>Infecting susceptible animals (D)</p> Signup and view all the answers

What was the significance of Chamberland's porcelain filter?

<p>It facilitated the isolation of filterable agents. (B)</p> Signup and view all the answers

Which technique helped to enhance the study of virus structures in the 1950s?

<p>Negative staining methods (D)</p> Signup and view all the answers

What was the first disease for which a filterable agent was identified?

<p>Tobacco mosaic disease (D)</p> Signup and view all the answers

Which scientist worked with cultured cell systems in the 1930s?

<p>Woodruff and Goodpasture (D)</p> Signup and view all the answers

Which bacterial disease was described by Walter Reed's team in 1901 as caused by a filterable agent?

<p>Yellow fever (C)</p> Signup and view all the answers

Why were antibiotics important in the development of single cell cultures?

<p>They controlled bacterial contamination. (D)</p> Signup and view all the answers

What did Antonie van Leeuwenhoek observe in his studies of water and organic materials?

<p>Animalcules, likely bacteria and protozoa (A)</p> Signup and view all the answers

How did Francesco Reddi contribute to the debate on spontaneous generation?

<p>He demonstrated that maggots only appeared on meat after flies laid eggs. (A)</p> Signup and view all the answers

What was John Needham's claim regarding microorganisms in boiled broth?

<p>Microorganisms can appear days after broth is boiled. (C)</p> Signup and view all the answers

What conclusion did Lazzaro Spallanzani reach regarding Needham's findings?

<p>Boiling broth eliminates all microorganisms. (D)</p> Signup and view all the answers

What significant advancement did Louis Pasteur contribute to microbiology?

<p>Introduction of pasteurization. (D)</p> Signup and view all the answers

What did Pasteur demonstrate about nutrient broth exposed to air?

<p>It gets contaminated by microorganisms from dust particles. (B)</p> Signup and view all the answers

What role did Robert Koch play in modern microbiology?

<p>He established the relationship between microbe and disease. (A)</p> Signup and view all the answers

What did Koch use to prove the pathogenicity of bacilli in anthrax?

<p>Injecting them into healthy mice. (C)</p> Signup and view all the answers

What was a foundational aspect of Pasteur’s germ theory of disease?

<p>Specific microorganisms can cause specific diseases. (A)</p> Signup and view all the answers

Which experiment was pivotal in disproving spontaneous generation in the 19th century?

<p>Spallanzani's boiling broth experiment. (C)</p> Signup and view all the answers

How did Pasteur combat spoilage during fermentation?

<p>By pasteurizing raw materials. (D)</p> Signup and view all the answers

What was a common misconception regarding spontaneous generation by proponents like Needham?

<p>Life can emerge from non-living matter. (B)</p> Signup and view all the answers

Which organism did Koch focus on isolating in his experiments for tuberculosis?

<p>Mycobacterium tuberculosis (A)</p> Signup and view all the answers

Which of the following statements best reflects Pasteur’s impact on food safety?

<p>He established pasteurization for microbial reduction. (B)</p> Signup and view all the answers

Flashcards

Origin of microbial life

Early life forms on Earth likely resembled anaerobic bacteria, indicating that prokaryotes evolved from simple life and diversified due to the availability of oxygen from photosynthesis.

What is Microbiology?

The study of microorganisms, especially bacteria, viruses, and fungi.

Spontaneous Generation

The theory that living organisms can arise from non-living matter.

Microorganisms

Organisms too small to be seen with the naked eye, often causing infectious diseases.

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Infectious Disease

An illness caused by a pathogen, often a microbe.

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Pathogen

The cause of an infectious disease, like bacteria or viruses.

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Characterizing Microorganisms

The process of discovering, isolating, and characterizing microorganisms.

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What is etiology?

The study of the cause of disease. It's how we understand what causes illness.

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Koch's Postulates

A technique used to determine the cause of a disease. It involves isolating and growing a suspected microorganism, infecting healthy animals, and re-isolating the microorganism from the sick animals.

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Etiology

The study of the cause of disease.

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Electron Microscope

A specialized type of microscope that uses electrons to create highly magnified images, revealing intricate details of viruses and other microscopic structures.

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Metal Shadowing Technique

A method used to visualize viruses, where a heavy metal is used to coat the virus, creating a shadow-like effect for better observation under an electron microscope.

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Negative Staining

A method used to observe viruses under the electron microscope, where a dense solution is used to contrast viruses with the background, allowing for easier viewing.

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Viruses

Tiny filterable agents, distinct from bacteria, that can cause a wide range of diseases, including rabies, smallpox, foot-and-mouth disease, and many more.

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Bacteriophages

Viruses that infect bacteria, potentially controlling bacterial populations and serving as a source for studying viral replication and bacterial genetics.

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Virus Cultivation

The process of growing and multiplying viruses in a controlled environment, often using living cells or tissues.

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Single-Cell Cultures

A type of cell culture that allows for the study of cell behavior and interactions, often involving the growth of cells in a single layer on a surface.

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Continuous Cell Lines

Lines of cells that can multiply indefinitely in a laboratory environment, providing a stable source for virus cultivation.

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Oncogenic Potential

The ability of a virus to cause the development of cancer.

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X-Ray Diffraction

The use of X-rays to study the structure of viruses, often revealing the intricate arrangements of viral components.

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What is Immunology?

The study of how the body responds to infectious agents like bacteria and viruses.

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What is a viral capsid?

A protein coat surrounding the genetic material (DNA or RNA) of a virus.

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What is X-ray crystallography?

The process of analyzing the structure of a molecule using X-ray diffraction patterns.

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What is Reverse Transcription?

The technique used to create a DNA copy of RNA, essential for understanding retroviruses.

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What are retroviruses?

Viruses that use reverse transcriptase to create DNA from their RNA genome.

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What is a viral oncogene?

A viral infection that can contribute to the development of cancer.

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What is Veterinary Microbiology?

The study of microorganisms that cause infectious diseases in animals.

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Antonie van Leeuwenhoek

A Dutch scientist who observed microscopic organisms (animalcules) using a microscope, contributing to the understanding of microorganisms but not resolving the debate about spontaneous generation.

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Maggots on Meat

The occurrence of maggots on decaying meat was initially thought to be evidence of spontaneous generation.

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Francesco Reddi

An Italian physician who refuted the idea of spontaneous generation of maggots by demonstrating that they only appeared when flies laid eggs on meat.

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John Needham

An English naturalist who challenged the view that spontaneous generation can occur, but his experiments were later shown to be unreliable due to insufficient boiling time.

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Lazzaro Spallanzani

An Italian biologist who repeated Needham's experiments, demonstrating that no organisms survived in broth boiled for a longer duration, contradicting spontaneous generation.

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Needham's Argument against Spallanzani

Spallanzani believed that air was necessary for all life, and that it was essential for spontaneous generation, whereas Spallanzani's experiments showed otherwise.

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Pasteurization

The process of heating raw materials to a certain temperature to kill contaminating microorganisms, a technique developed by Pasteur while working on fermentation.

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Louis Pasteur

A French chemist who definitively disproved spontaneous generation through definitive confirmation of Spallanzani's experiments and by demonstrating that contamination originates from microorganisms entering the environment.

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Germ Theory of Disease

The idea that specific microorganisms, such as bacteria, are responsible for causing specific diseases, a cornerstone of modern medicine.

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Robert Koch

A German physician who contributed significantly to microbiology by demonstrating the pathogenicity of anthrax bacilli and developing solid media for isolating and growing bacterial colonies.

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Microbiology

The science of studying microorganisms, such as bacteria, viruses, fungi, and protozoa.

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Culturing Microorganisms

A process where a specific microorganism is isolated and grown in a controlled environment, often used for research or diagnosis.

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Solid Media

A type of solid medium used for growing and isolating bacterial colonies, developed by Robert Koch.

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Study Notes

Early Life Forms and Infectious Disease

  • Earliest life forms resemble anaerobic bacteria
  • Prokaryotes evolved from primitive life forms
  • Oxygen availability, from photosynthesis, increased microbial diversity
  • Evolutionary sequence exists, though evidence is limited, showing microbial life and later development of eukaryotic cells
  • Traditional views on infectious diseases were based on spontaneous generation (from Greek/Roman scholars) and 'miasmas'.

Spontaneous Generation & Microscopy

  • Spontaneous generation, that life arises from non-living matter, was a long-held belief
  • Evidence existed from the appearance of maggots on decaying meat.
  • Improved scientific methods and microscopes challenged spontaneous generation.
  • Antonie van Leeuwenhoek observed "animalcules" (microorganisms) in water/fluids around 1675
  • Francesco Redi's experiments proved maggots originate from fly eggs(rather than meat), challenging spontaneous generation.
  • John Needham's experiments, claiming microorganisms could generate from boiled, cooled broth, was unreliable and later disproven.
  • Lazzaro Spallanzani repeated experiments & demonstrated no organisms grew in boiled, sealed broth, contradicting Needham's claims.
  • Louis Pasteur conclusively disproved spontaneous generation. His careful experiments showed that microorganisms in the air contaminated the broth.

Germ Theory and Koch's Postulates

  • Pasteur's and Koch's work confirmed microbial causes of infectious diseases
  • Pasteur's fermentation studies led to understanding of contamination by yeast
  • Pasteur developed pasteurization technique for preserving milk & foods.
  • Koch demonstrated pathogenicity of anthrax bacteria.
  • Koch developed solid media for isolation of bacterial colonies
  • Koch's postulates describe the process for proving that a microorganism is responsible for a disease.

Identifying Microbial Agents

  • By the late 19th century, many infectious diseases were identified as bacterial.
  • Pasteur & Koch's work identified the causative agent of Anthrax.
  • Koch’s and associates identified tuberculosis and typhoid fever causing agents.
  • Scientists isolated other serious infections, including glanders, gas gangrene, diphtheria, and dysentery.
  • Earlier techniques failed in cases like rabies, smallpox, foot-and-mouth, and rinderpest.
  • Smallpox and rabies vaccines were developed despite unclear etiology.
  • Development of a filter (by Pasteur’s associate) allowed isolation of filterable agents (viruses)
  • Tobacco mosaic disease was the first plant viral disease identified

Viruses & Microbiological Techniques

  • Dmitri Ivanovsky, followed by Martinus Beijerinck, demonstrated that certain diseases, like tobacco mosaic disease, could be transmitted with filtered extracts. This proved that the disease agents were smaller than bacteria (filterable).
  • 1898 Loeffler and Frosch identified foot-and-mouth virus.
  • 1901 Yellow fever virus described by Walter Reed and his team.
  • 1908 Ellerman and Bang linked a filterable agent to avian leukosis.
  • The discovery of bacteriophages
  • 1913 Steinhardt and colleagues grew vaccinia virus
  • Development of cell cultures (early 1950s) provided reliable virus cultivation, using techniques like trypsin and antibiotics to overcome contamination issues.

Virus Structure and Classification

  • TMV crystallization allowed closer study of viruses and their chemical composition.
  • Discovery of DNA structure and limited viral coding capacity led to proposals about the nucleic acid being encased in a protein shell.
  • 1962 Lwoff proposed a virus classification system (based on nucleic acid type, symmetry, envelope, and dimensions)
  • 1970 reverse transcriptase discovery, and complementary DNA production had major impact on understanding retrovirus replication and molecular biology
  • Electron microscopy, in combination with metal shadowing, negative staining, and ultrathin sectioning, dramatically enhanced understanding of virus ultrastructure. X-ray diffraction helped to establish the molecular structure of viruses.

Modern Microbiology

  • Modern microbiology encompasses bacteria, fungi, viruses, and other microorganisms.
  • Veterinary microbiology focuses on animal infectious diseases
  • Immunology studies the host's reaction to infectious agents and is closely related to microbiology.

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