DNA Vectors and Restriction Enzymes Quiz 8

Which type of restriction enzyme requires two non-palindromic sequences with opposite orientation near each other on the same DNA molecule for recognition?

What is the typical length of fragments generated by restriction enzymes that recognize four nucleotide sequences in the human genome?

Which statement is TRUE about Type I restriction enzymes?

What is the significance of the Roman numeral in the name of a restriction enzyme?

What is the basis for the difference in fragment lengths generated by restriction enzymes recognizing different length sequences?

Which of the following is NOT a characteristic of type II restriction enzymes?

Why do various sequences digested by the same restriction enzyme yield fragments of various lengths?

What is the purpose of treating the vector-acceptor with phosphatase?

In the context of plasmid maps, what happens when the insert is oriented opposite to the CMV promoter?

What restriction enzyme is specifically mentioned as forming real clones through its restriction site?

Which of the following describes a potential product of self-ligation of a closed vector?

What does the term 'double symmetrically closed plasmid-acceptor' imply in plasmid cloning?

What is the purpose of adding a specific antibiotic to agar plates when growing bacteria?

How does restriction analysis confirm the proper cloning of a new gene?

What characteristic of DNA fragments affects their migration speed in an agarose gel?

What is the significance of using unique combinations of restriction enzymes in cloning?

What is the primary method used to analyze DNA fragment masses after enzymatic digestion?

What is the role of plasmids in bacterial cloning processes?

Why is it important to isolate plasmids from bacterial clones?

What outcome is expected from the transformation of bacteria using plasmids?

What can be concluded from a successful restriction analysis of a plasmid?

What does the burgundy color indicate on a plasmid map regarding restriction enzyme cleavage?

Which of the following statements about plasmid maps is correct?

When designing digestion for cloning a specific DNA fragment, which of the following is NOT a typical step?

In molecular cloning, what is the main purpose of using restriction enzymes?

What does analyzing electrophoresis results allow researchers to determine?

What can be inferred if a restriction enzyme has a 'double or multiple cleave' denoted in black on a plasmid map?

When planning a cloning strategy for a DNA fragment, what is the role of the acceptor plasmid?

What is the significance of the location marked with the enzyme name followed by a number on the plasmid map?

Which step is essential for recognizing the products of a cloning procedure?

In a molecular cloning experiment, what is often the first action taken with the donor plasmid?

To achieve the correct orientation for the OVA-GFP gene aligning with the CMV promoter (sub-point a), which restriction enzyme combinations can be considered?

To attain the opposite orientation of the OVA-GFP gene relative to the CMV promoter (sub-point b), which restriction enzyme combination is appropriate?

What cloning strategy is suggested if the restriction sites flanking the insert are unavailable?

Which restriction enzyme could be used for cloning the OVA-GFP insert using a single enzyme?

What type of cloning method is associated with using only one restriction enzyme?

When analyzing new plasmids constructed using a single enzyme cloning method, what aspect should be considered?

Which restriction enzyme combination(s) would result in the OVA-GFP gene being in the same orientation as the CMV promoter?

What is a potential limitation of using only one restriction enzyme for cloning?

What considerations are important when selecting restriction enzymes for cloning?

Which statement accurately reflects the relationship between the OVA-GFP gene and the CMV promoter in sub-point b?