Podcast
Questions and Answers
What is the key learning outcome of the topic 'Basics of transcription and bursting'?
What is the key learning outcome of the topic 'Basics of transcription and bursting'?
- Understanding the steps involved from DNA to mature mRNA (correct)
- Measuring transcription output using gel techniques
- Defining key transcription terms
- Understanding the dynamics of DNA replication
Which technique is used to measure transcription output and is based on reverse transcription?
Which technique is used to measure transcription output and is based on reverse transcription?
- Gel electrophoresis
- RT-PCR (correct)
- Northern blot
- RNA-seq
What do all these techniques (Northern blot, RT-PCR, RNA-seq) have in common?
What do all these techniques (Northern blot, RT-PCR, RNA-seq) have in common?
- They are based on gel electrophoresis
- They are population-based rather than single cell (correct)
- They provide single cell readouts of transcription
- They measure DNA replication
Which technique suffers from low sequencing depth due to low RNA amount per cell?
Which technique suffers from low sequencing depth due to low RNA amount per cell?
Which strand of DNA can be used for transcription depending on where the gene body is located?
Which strand of DNA can be used for transcription depending on where the gene body is located?
What term refers to the process by which viruses can transcribe their RNA back into DNA?
What term refers to the process by which viruses can transcribe their RNA back into DNA?
What removes intronic regions and enables a variety of protein isoforms to be made from a single gene?
What removes intronic regions and enables a variety of protein isoforms to be made from a single gene?
Which complex facilitates the process of splicing?
Which complex facilitates the process of splicing?
What is the function of U2 in the splicing process?
What is the function of U2 in the splicing process?
What is the cause of over 2,000,000 proteins in our body despite having only approximately 20,000 protein coding genes in our genome?
What is the cause of over 2,000,000 proteins in our body despite having only approximately 20,000 protein coding genes in our genome?
What can cause transcription deregulation, contributing to cancer?
What can cause transcription deregulation, contributing to cancer?
What can be targeted in cancer therapy to address deregulated splicing?
What can be targeted in cancer therapy to address deregulated splicing?
Which mode of targeting transcriptional regulators is mentioned as a potential approach for achieving comprehensive tumor control?
Which mode of targeting transcriptional regulators is mentioned as a potential approach for achieving comprehensive tumor control?
What function do antisense oligonucleotides (AONs) serve in cancer therapy?
What function do antisense oligonucleotides (AONs) serve in cancer therapy?
What is necessary to produce an in-frame coding mRNA?
What is necessary to produce an in-frame coding mRNA?
What can be targeted to achieve more comprehensive tumor control in cancer therapy?
What can be targeted to achieve more comprehensive tumor control in cancer therapy?
Which process removes intronic regions and stabilizes the pre-mRNA molecule?
Which process removes intronic regions and stabilizes the pre-mRNA molecule?
What contributes to the production of over 2,000,000 proteins from approximately 20,000 protein coding genes in our genome?
What contributes to the production of over 2,000,000 proteins from approximately 20,000 protein coding genes in our genome?
What is the role of Enhancers in transcription regulation?
What is the role of Enhancers in transcription regulation?
Which component is responsible for creating the transcription bubble during initiation of DNA transcription?
Which component is responsible for creating the transcription bubble during initiation of DNA transcription?
What is the function of PAF1 in transcription elongation?
What is the function of PAF1 in transcription elongation?
What happens when RNA polymerase II reaches the 3' UTR during transcription termination?
What happens when RNA polymerase II reaches the 3' UTR during transcription termination?
What is the purpose of polyadenylation in transcription termination?
What is the purpose of polyadenylation in transcription termination?
How does higher order genome organization into chromatin structure affect gene transcription?
How does higher order genome organization into chromatin structure affect gene transcription?
What is the function of NURF during transcription elongation?
What is the function of NURF during transcription elongation?
What is the role of TFIIH in transcription initiation?
What is the role of TFIIH in transcription initiation?
"Euchromatin vs heterochromatin" is associated with:
"Euchromatin vs heterochromatin" is associated with:
"In vivo regulation of each gene is tightly controlled by CREs and TREs". This statement implies that:
"In vivo regulation of each gene is tightly controlled by CREs and TREs". This statement implies that:
What does P-TEFb do during transcription elongation?
What does P-TEFb do during transcription elongation?
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