DNA Technology and Genomics Quiz
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Questions and Answers

What role does human erythropoietin (EPO) play in the body?

  • Stimulates the production of red blood cells. (correct)
  • Stimulates blood clotting.
  • Promotes insulin release.
  • Increases heart rate.
  • Which of the following is NOT a therapeutic hormone produced by DNA technology?

  • Human erythropoietin
  • Factor VIII (correct)
  • Human growth hormone
  • Insulin
  • What are the benefits of genetically modified (GM) organisms?

  • Resistance to herbicides and pests. (correct)
  • Higher levels of pesticides.
  • Reduced nutrient content.
  • Longer shelf life of products.
  • Which organism is commonly used as a vector for introducing new genes into plant cells?

    <p>Agrobacterium tumefaciens</p> Signup and view all the answers

    What is a major concern regarding genetically modified organisms?

    <p>Potential introduction of allergens into the food supply.</p> Signup and view all the answers

    Recombinant animals must be cloned to produce more animals with what?

    <p>The same traits.</p> Signup and view all the answers

    What technology is used for producing vaccines and harmless variants of pathogens?

    <p>Recombinant DNA technology.</p> Signup and view all the answers

    What type of organisms contain one or more genes introduced by artificial means?

    <p>Genetically modified organisms.</p> Signup and view all the answers

    What is the primary role of restriction enzymes in cloning?

    <p>To cut DNA at specific sequences</p> Signup and view all the answers

    Which type of vector is commonly used for cloning genes?

    <p>Plasmids</p> Signup and view all the answers

    What are sticky ends in the context of DNA cloning?

    <p>Single-stranded ends that facilitate the joining of DNA fragments</p> Signup and view all the answers

    What is the result of using DNA ligase in the cloning process?

    <p>It joins DNA fragments together</p> Signup and view all the answers

    What is a genomic library?

    <p>A collection of cloned DNA fragments from a target genome</p> Signup and view all the answers

    What occurs during the step of isolating plasmid DNA?

    <p>Plasmid DNA is separated from the bacterial genomic DNA</p> Signup and view all the answers

    What is the primary purpose of DNA profiling?

    <p>To compare genetic markers to determine if they are from the same individual</p> Signup and view all the answers

    What type of genomic library incorporates genomic DNA into bacteriophage DNA?

    <p>Bacteriophage library</p> Signup and view all the answers

    What is the purpose of treating plasmid DNA with a restriction enzyme?

    <p>To cut the plasmid, allowing for the insertion of the target gene</p> Signup and view all the answers

    What is a key feature of the Polymerase Chain Reaction (PCR) method?

    <p>It employs a three-step cycle to double the DNA amount</p> Signup and view all the answers

    During gel electrophoresis, how do DNA fragments behave in the gel?

    <p>Shorter fragments move more quickly through the gel matrix</p> Signup and view all the answers

    What role do primers play in the PCR process?

    <p>They provide a starting point for DNA synthesis</p> Signup and view all the answers

    Which of the following is NOT an advantage of PCR?

    <p>Ability to analyze all genetic markers in a sample</p> Signup and view all the answers

    What occurs when an electric current is applied during gel electrophoresis?

    <p>DNA molecules move toward the positive electrode from a negative start</p> Signup and view all the answers

    Why is it important to analyze noncoding regions when conducting DNA profiling?

    <p>They often show variation that helps distinguish individuals</p> Signup and view all the answers

    What is the primary goal when inserting a gene into a plasmid in genetic engineering?

    <p>To produce a desired trait or protein</p> Signup and view all the answers

    What role do plasmids play in gene cloning?

    <p>They serve as carriers for transporting DNA into cells.</p> Signup and view all the answers

    How do restriction enzymes contribute to DNA technology?

    <p>They cut DNA at specific sequences to allow for gene insertion.</p> Signup and view all the answers

    What is a key difference between cDNA libraries and genomic libraries?

    <p>cDNA libraries are made from RNA templates, while genomic libraries are made from DNA.</p> Signup and view all the answers

    What is one application of genetically modified organisms (GMOs) in agriculture?

    <p>Enhancing the nutritional value of crops.</p> Signup and view all the answers

    Which statement accurately describes the DNA profiling process?

    <p>DNA profiling includes amplification of DNA and separation of fragments by gel electrophoresis.</p> Signup and view all the answers

    How has DNA technology contributed to the production of insulin?

    <p>By inserting the human insulin gene into bacteria for mass production.</p> Signup and view all the answers

    What is one of the risks associated with genetically modified organisms?

    <p>Potential ecological impacts from unintentional gene transfer.</p> Signup and view all the answers

    What is the primary advantage of using BACs in genomic libraries?

    <p>They can accommodate large fragments of DNA for cloning.</p> Signup and view all the answers

    What is the primary purpose of the polymerase chain reaction (PCR)?

    <p>To amplify specific DNA sequences</p> Signup and view all the answers

    What is the function of DNA ligase in molecular biology?

    <p>To join DNA fragments together</p> Signup and view all the answers

    Which term refers to an organism that contains genes from a different species?

    <p>Transgenic organism</p> Signup and view all the answers

    What does the term genomics specifically refer to?

    <p>The study of whole sets of genes</p> Signup and view all the answers

    Which technique is used to separate and purify DNA fragments?

    <p>Gel electrophoresis</p> Signup and view all the answers

    What is the role of primers in the polymerase chain reaction?

    <p>To initiate the synthesis of new DNA strands</p> Signup and view all the answers

    Which term specifically describes repetitive DNA at the ends of eukaryotic chromosomes?

    <p>Telomeres</p> Signup and view all the answers

    Which is a function of a nucleic acid probe in molecular biology?

    <p>To hybridize with complementary DNA sequences</p> Signup and view all the answers

    What is the primary function of restriction enzymes in nature?

    <p>To protect bacterial cells against foreign DNA</p> Signup and view all the answers

    How is a genomic library analogous to editing a sentence?

    <p>Both require adding spaces between specific letters</p> Signup and view all the answers

    What does a cDNA library indicate about a cell's genome?

    <p>It indicates which portions of the genome are active at a given time</p> Signup and view all the answers

    In what way is a nucleic acid probe's functionality distinctive compared to Internet search methods?

    <p>It relies solely on matching the exact sequence without variations</p> Signup and view all the answers

    Which statement accurately reflects a common misconception about genetic engineering?

    <p>All genetically modified organisms are harmful to health</p> Signup and view all the answers

    What best describes the process of studying a cDNA library?

    <p>It focuses on proteins that are synthesized by RNA</p> Signup and view all the answers

    How can the public perception of genetic engineering be characterized?

    <p>It is influenced by varying degrees of unease and misinformation</p> Signup and view all the answers

    Why might someone use a phrase search when looking for song lyrics online?

    <p>Because song titles are often not reliable keywords</p> Signup and view all the answers

    Study Notes

    DNA Technology and Genomics

    • DNA technology is important for forensics, producing medical/industrial products, investigating evolutionary relationships, and biological research.
    • Biotechnology involves manipulating organisms to make useful products. DNA technology uses modern techniques to manipulate genetic material.
    • Gene cloning produces identical copies of a gene. Recombinant DNA is formed by joining nucleotide sequences from different sources.
    • Plasmids, phages, and BACs are used to construct genomic libraries. A cDNA library is different from a genomic library.
    • Restriction enzymes cut DNA at specific sequences, creating sticky ends for joining with other fragments.
    • DNA ligase joins DNA fragments together.
    • Nucleic acid probes identify specific genes in libraries.
    • Genetically modified organisms (GMOs) are used to mass-produce proteins.
    • Bacteria, yeast, and mammalian cells are used for mass production of proteins.
    • GMOs transform agriculture by creating crops resistant to herbicides and pests.
    • Benefits include increased nutrition and disease resistance in plants. Risks need safeguarding against pathogens.
    • DNA profiling analyzes genetic markers to determine if DNA comes from the same individual.
    • Polymerase Chain Reaction (PCR) rapidly amplifies DNA sequences.
    • Gel electrophoresis separates DNA and proteins by size and charge.

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    Description

    Test your knowledge on DNA technology and genomics with this quiz. Explore concepts such as gene cloning, recombinant DNA, and the use of GMOs in biotechnology. Understand the significance of techniques like restriction enzymes and genomic libraries.

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