Podcast
Questions and Answers
What is the approximate size range of plasmids in terms of base pairs (bp)?
What is the approximate size range of plasmids in terms of base pairs (bp)?
- 10^3 - 10^5 bp (correct)
- 10^5 - 10^7 bp
- 10^2 - 10^4 bp
- 10^4 - 10^6 bp
Which type of enzyme cleaves DNA strands at specific DNA sequences?
Which type of enzyme cleaves DNA strands at specific DNA sequences?
- Ligase
- Endonuclease (correct)
- Helicase
- Polymerase
Which enzyme is responsible for hydrolyzing the phosphodiester bond within a polynucleotide chain?
Which enzyme is responsible for hydrolyzing the phosphodiester bond within a polynucleotide chain?
- Restriction enzyme
- Polymerase
- Endonuclease (correct)
- Exonuclease
What is the approximate length of the E.coli chromosome DNA?
What is the approximate length of the E.coli chromosome DNA?
Why is DNA supercoiling important in bacterial cells?
Why is DNA supercoiling important in bacterial cells?
What property makes poly-deoxyribonucleotides suitable for long-term storage of genetic information?
What property makes poly-deoxyribonucleotides suitable for long-term storage of genetic information?
What was the turning point in DNA development that gave rise to modern molecular biology?
What was the turning point in DNA development that gave rise to modern molecular biology?
In the structure of DNA, where are the phosphate and sugar groups located?
In the structure of DNA, where are the phosphate and sugar groups located?
What type of DNA structure twists in a clockwise direction?
What type of DNA structure twists in a clockwise direction?
Which model of DNA structure was proposed by Watson and Crick?
Which model of DNA structure was proposed by Watson and Crick?
What is the main function of DNA polymerases?
What is the main function of DNA polymerases?
What is the purpose of telomere maintenance in DNA?
What is the purpose of telomere maintenance in DNA?
What are the three families of LINE elements?
What are the three families of LINE elements?
Which retroelement is known for lacking autonomous transposition capability?
Which retroelement is known for lacking autonomous transposition capability?
What is the most common SINE element?
What is the most common SINE element?
Why can the insertion of LINE1, Alu, and SINE sequences into coding regions of genes lead to genetic diseases?
Why can the insertion of LINE1, Alu, and SINE sequences into coding regions of genes lead to genetic diseases?
Under what conditions has transposition of SINE and LINE elements been observed?
Under what conditions has transposition of SINE and LINE elements been observed?
What is the status of LINE1 sequences with respect to active transposition in the human genome?
What is the status of LINE1 sequences with respect to active transposition in the human genome?
What types of base-to-base interactions determine the stability of the DNA double helix?
What types of base-to-base interactions determine the stability of the DNA double helix?
What initiated a new era of biology according to Watson and Crick's 1953 paper in Nature?
What initiated a new era of biology according to Watson and Crick's 1953 paper in Nature?
Where do deep major grooves occur in the DNA double helix?
Where do deep major grooves occur in the DNA double helix?
What did Watson and Crick want to suggest in their 1953 paper?
What did Watson and Crick want to suggest in their 1953 paper?
What kind of pairing do complementary nucleotide strands exhibit in the DNA double helix?
What kind of pairing do complementary nucleotide strands exhibit in the DNA double helix?
Where do proteins that bind DNA interact specifically according to the text?
Where do proteins that bind DNA interact specifically according to the text?
What is the main function of DNA helicase in the replication process?
What is the main function of DNA helicase in the replication process?
Which enzyme is responsible for synthesizing a short RNA primer during replication?
Which enzyme is responsible for synthesizing a short RNA primer during replication?
What is the function of single-strand binding proteins (SSB) during replication?
What is the function of single-strand binding proteins (SSB) during replication?
Why is DNA Polymerase unable to initiate the synthesis of a new DNA strand from scratch?
Why is DNA Polymerase unable to initiate the synthesis of a new DNA strand from scratch?
What are the small DNA fragments on the lagging strand called?
What are the small DNA fragments on the lagging strand called?
Which enzyme initiates replication in eukaryotes by synthesizing a short primer?
Which enzyme initiates replication in eukaryotes by synthesizing a short primer?
