DNA Structure and History Quiz
21 Questions
2 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the percentage composition of thymine (T) in the DNA sample from species X?

  • 18%
  • 64%
  • 32% (correct)
  • 36%
  • What is the total percentage of guanine (G) in the DNA sample from species Y?

  • 66%
  • 33% (correct)
  • 34%
  • 17%
  • Which species is more likely to be a thermophilic bacterium based on DNA stability?

  • Both species are equally thermophilic
  • Neither species can be thermophilic
  • Species X due to higher adenine content
  • Species Y due to higher G+C content (correct)
  • Which assumption is made about the DNA of both species?

    <p>They are double-stranded DNA (B)</p> Signup and view all the answers

    Why does species Y’s DNA have a higher melting temperature?

    <p>It has a higher G+C content. (C)</p> Signup and view all the answers

    What is the significance of base-stacking interactions in DNA stability?

    <p>They are stronger than those between successive G≡C pairs. (C)</p> Signup and view all the answers

    Which form of DNA is considered the most stable under physiological conditions?

    <p>B-form DNA (B)</p> Signup and view all the answers

    What structural feature distinguishes palindromic DNA sequences?

    <p>They are identical when read backward. (C)</p> Signup and view all the answers

    What type of structure can be formed by palindromic DNA sequences due to intrastrand base pairing?

    <p>Hairpin structures (B)</p> Signup and view all the answers

    Which of the following statements about mirror repeats is true?

    <p>They cannot form cruciform structures. (A)</p> Signup and view all the answers

    Which base pair combination exhibits a triple bond?

    <p>G and C (A)</p> Signup and view all the answers

    What is characteristic of the A-form DNA structure compared to B-form DNA?

    <p>It typically forms under dehydrated conditions. (C)</p> Signup and view all the answers

    What type of interactions primarily hold together the DNA double helix?

    <p>Hydrogen bonds (B)</p> Signup and view all the answers

    Who first isolated and characterized DNA?

    <p>Friedrich Miescher (C)</p> Signup and view all the answers

    What experiment demonstrated that DNA is the genetic material?

    <p>Avery, MacLeod, and McCarty experiments (D)</p> Signup and view all the answers

    According to Chargaff's rules, which relationship is correct?

    <p>A = T and G = C (A), A + G = T + C (C)</p> Signup and view all the answers

    What did Watson and Crick propose in 1953 regarding DNA?

    <p>DNA has a right-handed double helix structure (D)</p> Signup and view all the answers

    Chargaff's research indicated that the base composition of DNA is:

    <p>Different among different organisms (B)</p> Signup and view all the answers

    What feature of DNA strands is described as 'antiparallel'?

    <p>Strands run in opposite directions (D)</p> Signup and view all the answers

    Which of the following statements about the discovery of DNA's role in genetics is true?

    <p>DNA was confirmed to be the genetic material after 1952 (C)</p> Signup and view all the answers

    The transformation of which bacterium by DNA was demonstrated by Avery and colleagues?

    <p>Streptococcus pneumoniae (B)</p> Signup and view all the answers

    Flashcards

    Chargaff's Rule

    In a double-stranded DNA molecule, adenine (A) always pairs with thymine (T) and guanine (G) always pairs with cytosine (C).

    Base Pairing Ratios

    The percentage of adenine (A) in a DNA molecule is equal to the percentage of thymine (T), and the percentage of guanine (G) is equal to the percentage of cytosine (C).

    Melting Temperature (Tm)

    The temperature at which a double-stranded DNA molecule separates into single strands.

    G-C Content and Tm

    A DNA molecule with a higher proportion of guanine (G) and cytosine (C) base pairs will have a higher melting temperature.

    Signup and view all the flashcards

    Thermophilic Bacteria

    Organisms that thrive in high temperatures (like hot springs).

    Signup and view all the flashcards

    Base Pairing in DNA

    Adenine (A) always pairs with thymine (T), and guanine (G) always pairs with cytosine (C) in a DNA molecule. These pairs are held together by hydrogen bonds.

    Signup and view all the flashcards

    DNA Double Helix Stability

    The double helix structure of DNA is stabilized by hydrogen bonds between complementary base pairs (A-T and G-C) and interactions between stacked bases.

    Signup and view all the flashcards

    G≡C vs A═T Stability

    DNA duplexes with a higher G≡C content are more stable than those with a higher A═T content. This is due to the stronger base-stacking interactions between G≡C pairs.

    Signup and view all the flashcards

    B-form DNA

    The B-form is the most common and physiologically relevant form of DNA. It's a right-handed helix with a smooth, uniform appearance.

    Signup and view all the flashcards

    A-form DNA

    A-DNA is another form of DNA, often found in dehydrated conditions or when DNA is bound to proteins. It's a wider and more compact helix.

    Signup and view all the flashcards

    Z-form DNA

    Z-DNA is a left-handed helix with a zig-zag appearance. It's found in certain sequences, often with alternating purines and pyrimidines.

    Signup and view all the flashcards

    Palindrome in DNA

    A palindrome is a sequence of nucleotides that reads the same forward and backward on both strands. These sequences can form hairpin or cruciform structures.

    Signup and view all the flashcards

    Mirror Repeat in DNA

    A mirror repeat is a sequence of nucleotides that has an inverted, self-complementary sequence within the same strand, but not in the opposite orientation. It cannot form hairpin or cruciform structures.

    Signup and view all the flashcards

    What is DNA?

    The substance that carries genetic information in living organisms. It is a long molecule made up of two strands of nucleotides twisted around each other to form a double helix.

    Signup and view all the flashcards

    Who first discovered DNA?

    Friedrich Miescher was a Swiss physician who first isolated and characterized DNA in 1869. He initially called it "nuclein" due to its phosphorus-containing nature.

    Signup and view all the flashcards

    Who proved DNA is the genetic material?

    Oswald Avery, Colin MacLeod, and Maclyn McCarty conducted groundbreaking experiments in the 1940s to prove that DNA, not protein, was the genetic material. They demonstrated that DNA from a virulent strain of bacteria could transform a nonvirulent strain into a virulent one.

    Signup and view all the flashcards

    Who definitively proved DNA carries genetic information?

    Alfred Hershey and Martha Chase conducted experiments in 1952 that definitively confirmed DNA as the carrier of genetic information. They used radioactive isotopes to label DNA and protein in viruses, showing that only DNA was transferred to the host cells during infection.

    Signup and view all the flashcards

    What are Chargaff's rules?

    Erwin Chargaff's rules state that in DNA, the amount of adenine (A) always equals the amount of thymine (T), and the amount of guanine (G) always equals the amount of cytosine (C). This led to the understanding that the two strands of DNA are complementary.

    Signup and view all the flashcards

    Who proposed the double helix model of DNA?

    James Watson and Francis Crick proposed the double helix model of DNA in 1953. This model describes DNA as two strands of nucleotides wound around each other, forming a twisted ladder-like structure.

    Signup and view all the flashcards

    What does antiparallel mean in DNA?

    In the double helix structure of DNA, the two strands run in opposite directions, termed antiparallel. This means that one strand runs from 5' to 3', while the other runs from 3' to 5'.

    Signup and view all the flashcards

    How are the two strands of DNA held together?

    The two strands of DNA are held together by hydrogen bonds between the bases. Adenine pairs with thymine (A-T) through two hydrogen bonds, while guanine pairs with cytosine (G-C) through three hydrogen bonds.

    Signup and view all the flashcards

    Study Notes

    DNA Structure and History

    • DNA was first isolated and characterized by Friedrich Miescher in 1869. He called the phosphorus-containing substance "nuclein."
    • In the 1940s, Oswald Avery, Colin MacLeod, and Maclyn McCarty provided compelling evidence that DNA is the genetic material.
    • They demonstrated that DNA from a virulent strain of bacteria could transform a non-virulent strain.
    • Experiments by Hershey and Chase in 1952 further confirmed that DNA, not protein, carries genetic information.

    Chargaff's Rule

    • Erwin Chargaff's research revealed consistent ratios of DNA bases across different species and tissues.
    • The number of adenine residues equals the number of thymine residues (A=T), and the number of guanine residues equals the number of cytosine residues (G=C).
    • A + G = T + C
    • This principle is key to understanding DNA structure.

    DNA Structure

    • James Watson and Francis Crick developed a three-dimensional model of DNA in 1953, based on accumulated information about DNA.
    • DNA consists of two helical chains wound around the same axis to form a right-handed double helix.
    • The double helix is characterized by a major and minor groove.

    Complementary Strands

    • The two antiparallel polynucleotide chains of double-helical DNA have complementary base sequences.
    • Wherever adenine (A) is present in one strand, thymine (T) is found in the other.
    • Wherever guanine (G) is present, cytosine (C) occurs.

    Hydrogen Bonding

    • Hydrogen bonds between complementary base pairs hold the DNA double helix together, along with base-stacking interactions.
    • Base-stacking interactions between successive G≡C base pairs is stronger than between successive A=T.
    • DNA duplexes with higher G-C content are more stable.

    Different Three-Dimensional Forms

    • The Watson-Crick structure is also referred to as B-form DNA, which is the most common and stable form under normal conditions.
    • A and Z-forms are also possible three-dimensional configurations of DNA but are less common than the B-form.

    Palindromes

    • Palindromes are DNA sequences that read the same forward and backward.
    • Inverted repeats can form hairpin or cruciform structures.
    • Mirror repeats do not have complementary sequences within the same strand.

    Hairpins and Cruciforms

    • Palindromic sequences can form hairpin or cruciform structures within a single strand or involve both strands of a duplex.
    • Asymmetric sequences can pair with their complementary sequence within the same or the complementary strands

    DNA Base Pairs and Stability

    • In DNA samples of two different bacteria, species X and Y, the proportions of adenine bases vary.
    • The higher the G+C content of a DNA molecule, the higher the melting temperature, making it more stable at higher temperatures.
    • Species with a higher G+C content are more likely thermophilic bacteria.

    Studying That Suits You

    Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

    Quiz Team

    Related Documents

    Description

    Test your knowledge on the history and structure of DNA. This quiz covers significant milestones in DNA research, including the contributions of key scientists like Miescher, Chargaff, and Watson & Crick. Explore the fundamental principles that define the genetic material of life.

    More Like This

    DNA Structure and Components Quiz
    10 questions
    DNA Structure Review Flashcards
    15 questions

    DNA Structure Review Flashcards

    ManeuverableForgetMeNot2590 avatar
    ManeuverableForgetMeNot2590
    Nucleic Acids and Chargaff's Rule
    13 questions
    Use Quizgecko on...
    Browser
    Browser