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Questions and Answers

What role does ligase play during DNA replication?

  • It unwinds the double helix.
  • It synthesizes RNA primers.
  • It checks for errors in the newly synthesized DNA.
  • It joins Okazaki fragments. (correct)
  • Which nitrogenous bases form two hydrogen bonds when paired together?

  • Adenine and Cytosine
  • Guanine and Thymine
  • Adenine and Thymine (correct)
  • Cytosine and Guanine
  • What is the primary location of DNA in eukaryotic cells?

  • Cytoplasm
  • Ribosomes
  • Nucleus (correct)
  • Mitochondria
  • During which part of the cell cycle does DNA replication occur?

    <p>S phase</p> Signup and view all the answers

    What is the first step in transcription?

    <p>The DNA double helix unwinds.</p> Signup and view all the answers

    Which component is NOT part of the backbone of the DNA strand?

    <p>Nitrogenous bases</p> Signup and view all the answers

    What process follows DNA transcription to produce proteins?

    <p>Translation</p> Signup and view all the answers

    What is the function of RNA polymerase during transcription?

    <p>It synthesizes mRNA.</p> Signup and view all the answers

    Which bases form three hydrogen bonds when paired together?

    <p>Cytosine and Guanine</p> Signup and view all the answers

    Which molecule is primarily responsible for proofreading during DNA replication?

    <p>DNA polymerase</p> Signup and view all the answers

    What is the primary purpose of the polymerase chain reaction (PCR) in DNA profiling?

    <p>To create millions of copies of specific DNA regions</p> Signup and view all the answers

    Which enzyme is primarily responsible for transcription in eukaryotic cells?

    <p>RNA polymerase</p> Signup and view all the answers

    In the context of protein synthesis, what occurs during the termination phase?

    <p>A stop codon signals the end of translation</p> Signup and view all the answers

    What distinguishes point mutations from frameshift mutations?

    <p>Point mutations involve changes in single nucleotides</p> Signup and view all the answers

    How does gel electrophoresis contribute to DNA profiling?

    <p>By creating unique patterns of DNA based on size</p> Signup and view all the answers

    What is a common application of DNA profiling in forensic science?

    <p>Matching DNA from crime scenes to suspects</p> Signup and view all the answers

    Which of the following correctly contrasts DNA replication with transcription?

    <p>DNA replication results in two identical DNA molecules</p> Signup and view all the answers

    What is the effect of silent mutations on protein synthesis?

    <p>They do not change the amino acid sequence</p> Signup and view all the answers

    What role does biotechnology, such as CRISPR, have in relation to genetic mutations?

    <p>It aims to correct mutations and treat genetic diseases</p> Signup and view all the answers

    Which characteristic of DNA profiling allows it to identify individuals uniquely?

    <p>Examining specific regions that vary greatly among individuals</p> Signup and view all the answers

    What structural feature makes DNA stable during the replication process?

    <p>Hydrogen bonds between complementary bases</p> Signup and view all the answers

    Which enzyme is involved in adding nucleotides during DNA elongation?

    <p>DNA polymerase</p> Signup and view all the answers

    What is the primary function of helicase during DNA replication?

    <p>To unwind the double helix</p> Signup and view all the answers

    During which process is mRNA modified to enhance stability?

    <p>Processing</p> Signup and view all the answers

    What type of bond exists between adenine and thymine in DNA?

    <p>Hydrogen bonds</p> Signup and view all the answers

    Which phase of the cell cycle is specifically designated for DNA replication?

    <p>S phase</p> Signup and view all the answers

    What is the role of ligase in DNA replication?

    <p>Joins Okazaki fragments</p> Signup and view all the answers

    Which type of mutation results in a shift of the entire reading frame during protein synthesis?

    <p>Frameshift mutation</p> Signup and view all the answers

    What is the main consequence of errors during DNA replication that are not corrected?

    <p>Mutations</p> Signup and view all the answers

    What component is removed from mRNA during its processing phase?

    <p>Introns</p> Signup and view all the answers

    What happens during the initiation phase of translation?

    <p>The ribosome binds to the mRNA, signaling the start of protein synthesis</p> Signup and view all the answers

    Which step in DNA profiling involves cutting DNA into fragments?

    <p>Fragmentation</p> Signup and view all the answers

    In which scenario would a frameshift mutation most likely occur?

    <p>A nucleotide is deleted from the sequence</p> Signup and view all the answers

    Which type of mutation introduces a premature stop codon?

    <p>Nonsense Mutations</p> Signup and view all the answers

    What is the first step in DNA profiling?

    <p>Sample Collection</p> Signup and view all the answers

    What distinguishes the end products of DNA replication and transcription?

    <p>Transcription results in a single mRNA strand, while replication creates two identical DNA molecules</p> Signup and view all the answers

    Which enzyme is primarily responsible for linking amino acids during protein synthesis?

    <p>Ribosome</p> Signup and view all the answers

    What is the primary application of DNA profiling in forensic science?

    <p>Matching crime scene DNA to suspects</p> Signup and view all the answers

    What feature characterizes the process of gel electrophoresis in DNA profiling?

    <p>It separates DNA fragments based on size</p> Signup and view all the answers

    Which of the following mutations does NOT affect the amino acid sequence?

    <p>Silent Mutations</p> Signup and view all the answers

    What is the role of deoxyribose sugar in DNA?

    <p>It forms part of the backbone of the DNA strand.</p> Signup and view all the answers

    Which step in DNA replication involves adding complementary nucleotides?

    <p>Elongation</p> Signup and view all the answers

    During which process does the DNA double helix unwind?

    <p>Initiation</p> Signup and view all the answers

    What type of bond connects adenine to thymine?

    <p>Hydrogen bond</p> Signup and view all the answers

    What is the primary function of RNA polymerase in protein synthesis?

    <p>To synthesize mRNA from a DNA template.</p> Signup and view all the answers

    Which description best defines the role of ligase in DNA replication?

    <p>Joining Okazaki fragments on the leading strand.</p> Signup and view all the answers

    Which step of transcription is primarily responsible for the stability of mRNA?

    <p>Processing</p> Signup and view all the answers

    What is the key difference between DNA replication and transcription?

    <p>Replication creates a complete set of DNA, while transcription creates RNA from DNA.</p> Signup and view all the answers

    What is a significant characteristic of the polypeptide formation step in protein synthesis?

    <p>It links amino acids through covalent bonds.</p> Signup and view all the answers

    In the context of DNA profiling, which step is crucial for creating a unique banding pattern in gel electrophoresis?

    <p>Fragmentation</p> Signup and view all the answers

    How do nonsense mutations affect protein synthesis?

    <p>They introduce a premature stop codon.</p> Signup and view all the answers

    What distinguishes transcription from DNA replication?

    <p>DNA replication involves the copying of entire DNA molecules.</p> Signup and view all the answers

    Which type of mutation is characterized by a single nucleotide change?

    <p>Point Mutation</p> Signup and view all the answers

    What application of DNA profiling is typically used to establish biological relationships?

    <p>Paternity Testing</p> Signup and view all the answers

    What impact do silent mutations usually have on the amino acid sequence of a protein?

    <p>They do not change the amino acid sequence.</p> Signup and view all the answers

    What is the primary role of RNA polymerase during the transcription process?

    <p>To synthesize the mRNA strand.</p> Signup and view all the answers

    Which of the following best describes mutations that cause severe changes to the protein's function?

    <p>Missense Mutations</p> Signup and view all the answers

    During which phase does mRNA leave the nucleus and begin its role in protein synthesis?

    <p>Exit Phase</p> Signup and view all the answers

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