DNA Sequencing using Dideoxynucleotides
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Questions and Answers

What is the main purpose of gel electrophoresis in DNA sequencing?

  • To amplify specific DNA regions
  • To determine the DNA sequence based on the colored dye
  • To incorporate ddNTPs at every position of the DNA strand
  • To separate DNA strands based on length (correct)
  • In polymerase chain reaction (PCR), what is the role of Taq polymerase?

  • Separating DNA strands based on length
  • Determining the DNA sequence
  • Synthesizing new DNA strands from nucleotides (correct)
  • Adding ddNTPs to the DNA chain
  • Why are primers in PCR required to have high GC content and either a G or C at each end?

  • To incorporate ddNTPs at every position of the DNA strand
  • To separate DNA strands based on length
  • To amplify specific DNA regions efficiently (correct)
  • To determine the DNA sequence accurately
  • What is the purpose of ddNTPs in Sanger sequencing?

    <p>Determining the DNA sequence accurately</p> Signup and view all the answers

    Why is Taq polymerase preferred for PCR?

    <p>It is very heat-stable and most active around 70°C</p> Signup and view all the answers

    Which technique uses silica as the stationary phase and a nonpolar liquid as the mobile phase?

    <p><em>Liquid Chromatography</em></p> Signup and view all the answers

    What is the role of DNA Primase in DNA replication?

    <p>Catalyze the synthesis of the RNA primer</p> Signup and view all the answers

    Which enzyme removes the RNA primer at the end of the DNA strand during replication?

    <p>DNA Polymerase</p> Signup and view all the answers

    What is the function of Okazaki fragments in DNA replication?

    <p>Short, newly synthesized DNA fragments on the lagging strand</p> Signup and view all the answers

    In eukaryotes, which type of DNA polymerase is primarily used during DNA replication?

    <p>Pol d</p> Signup and view all the answers

    What is the function of DNA Ligase in DNA replication?

    <p>Join DNA strands together by forming phosphodiester bonds</p> Signup and view all the answers

    Which enzyme reads the template 3’ ® 5’ and synthesizes the new DNA strand 5’ ® 3’ during DNA replication?

    <p>DNA Polymerase</p> Signup and view all the answers

    In the context of molecular biology techniques, which process involves the separation of DNA fragments based on size and charge?

    <p>Gel electrophoresis</p> Signup and view all the answers

    Which technique is used to amplify a specific region of DNA through repeated cycles of denaturation, annealing, and extension?

    <p>Polymerase chain reaction</p> Signup and view all the answers

    What is the purpose of Sanger sequencing in molecular biology?

    <p>To determine the sequence of nucleotides in a DNA fragment</p> Signup and view all the answers

    Which step in the polymerase chain reaction (PCR) process involves the addition of short DNA sequences that are complementary to the target region?

    <p>Annealing</p> Signup and view all the answers

    What is the primary function of primers in the polymerase chain reaction (PCR) technique?

    <p>To initiate DNA synthesis at a specific region</p> Signup and view all the answers

    Which technique involves designing short pieces of single-stranded DNA that are complementary to the sequences flanking the target region in DNA amplification?

    <p>Primer design</p> Signup and view all the answers

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