18 Questions
What is the main purpose of gel electrophoresis in DNA sequencing?
To separate DNA strands based on length
In polymerase chain reaction (PCR), what is the role of Taq polymerase?
Synthesizing new DNA strands from nucleotides
Why are primers in PCR required to have high GC content and either a G or C at each end?
To amplify specific DNA regions efficiently
What is the purpose of ddNTPs in Sanger sequencing?
Determining the DNA sequence accurately
Why is Taq polymerase preferred for PCR?
It is very heat-stable and most active around 70°C
Which technique uses silica as the stationary phase and a nonpolar liquid as the mobile phase?
Liquid Chromatography
What is the role of DNA Primase in DNA replication?
Catalyze the synthesis of the RNA primer
Which enzyme removes the RNA primer at the end of the DNA strand during replication?
DNA Polymerase
What is the function of Okazaki fragments in DNA replication?
Short, newly synthesized DNA fragments on the lagging strand
In eukaryotes, which type of DNA polymerase is primarily used during DNA replication?
Pol d
What is the function of DNA Ligase in DNA replication?
Join DNA strands together by forming phosphodiester bonds
Which enzyme reads the template 3’ ® 5’ and synthesizes the new DNA strand 5’ ® 3’ during DNA replication?
DNA Polymerase
In the context of molecular biology techniques, which process involves the separation of DNA fragments based on size and charge?
Gel electrophoresis
Which technique is used to amplify a specific region of DNA through repeated cycles of denaturation, annealing, and extension?
Polymerase chain reaction
What is the purpose of Sanger sequencing in molecular biology?
To determine the sequence of nucleotides in a DNA fragment
Which step in the polymerase chain reaction (PCR) process involves the addition of short DNA sequences that are complementary to the target region?
Annealing
What is the primary function of primers in the polymerase chain reaction (PCR) technique?
To initiate DNA synthesis at a specific region
Which technique involves designing short pieces of single-stranded DNA that are complementary to the sequences flanking the target region in DNA amplification?
Primer design
Learn about DNA sequencing using dideoxynucleotides (ddNTPs) and gel electrophoresis to determine the sequence of a DNA strand. Explore how the lack of a 3’ hydroxyl group in ddNTPs halts DNA synthesis and how different colored dyes are used to identify the terminating base. Understand the process of sequencing DNA by separating strands based on length.
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