Podcast
Questions and Answers
What is the main purpose of gel electrophoresis in DNA sequencing?
What is the main purpose of gel electrophoresis in DNA sequencing?
- To amplify specific DNA regions
- To determine the DNA sequence based on the colored dye
- To incorporate ddNTPs at every position of the DNA strand
- To separate DNA strands based on length (correct)
In polymerase chain reaction (PCR), what is the role of Taq polymerase?
In polymerase chain reaction (PCR), what is the role of Taq polymerase?
- Separating DNA strands based on length
- Determining the DNA sequence
- Synthesizing new DNA strands from nucleotides (correct)
- Adding ddNTPs to the DNA chain
Why are primers in PCR required to have high GC content and either a G or C at each end?
Why are primers in PCR required to have high GC content and either a G or C at each end?
- To incorporate ddNTPs at every position of the DNA strand
- To separate DNA strands based on length
- To amplify specific DNA regions efficiently (correct)
- To determine the DNA sequence accurately
What is the purpose of ddNTPs in Sanger sequencing?
What is the purpose of ddNTPs in Sanger sequencing?
Why is Taq polymerase preferred for PCR?
Why is Taq polymerase preferred for PCR?
Which technique uses silica as the stationary phase and a nonpolar liquid as the mobile phase?
Which technique uses silica as the stationary phase and a nonpolar liquid as the mobile phase?
What is the role of DNA Primase in DNA replication?
What is the role of DNA Primase in DNA replication?
Which enzyme removes the RNA primer at the end of the DNA strand during replication?
Which enzyme removes the RNA primer at the end of the DNA strand during replication?
What is the function of Okazaki fragments in DNA replication?
What is the function of Okazaki fragments in DNA replication?
In eukaryotes, which type of DNA polymerase is primarily used during DNA replication?
In eukaryotes, which type of DNA polymerase is primarily used during DNA replication?
What is the function of DNA Ligase in DNA replication?
What is the function of DNA Ligase in DNA replication?
Which enzyme reads the template 3’ ® 5’ and synthesizes the new DNA strand 5’ ® 3’ during DNA replication?
Which enzyme reads the template 3’ ® 5’ and synthesizes the new DNA strand 5’ ® 3’ during DNA replication?
In the context of molecular biology techniques, which process involves the separation of DNA fragments based on size and charge?
In the context of molecular biology techniques, which process involves the separation of DNA fragments based on size and charge?
Which technique is used to amplify a specific region of DNA through repeated cycles of denaturation, annealing, and extension?
Which technique is used to amplify a specific region of DNA through repeated cycles of denaturation, annealing, and extension?
What is the purpose of Sanger sequencing in molecular biology?
What is the purpose of Sanger sequencing in molecular biology?
Which step in the polymerase chain reaction (PCR) process involves the addition of short DNA sequences that are complementary to the target region?
Which step in the polymerase chain reaction (PCR) process involves the addition of short DNA sequences that are complementary to the target region?
What is the primary function of primers in the polymerase chain reaction (PCR) technique?
What is the primary function of primers in the polymerase chain reaction (PCR) technique?
Which technique involves designing short pieces of single-stranded DNA that are complementary to the sequences flanking the target region in DNA amplification?
Which technique involves designing short pieces of single-stranded DNA that are complementary to the sequences flanking the target region in DNA amplification?
