DNA Sequencing Methods

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Questions and Answers

What temperature is used during the denaturation step of the cycle sequencing reaction?

95°C

How is the clean-up step performed in the cycle sequencing reaction?

By removing non-DNA materials through EtOH/EDTA precipitation

What is the purpose of the cycle sequencing reaction?

To create a population of DNA molecules, each terminating at a different site

What is the purpose of the DNA sequencing technique?

<p>To determine the order of four bases: adenine (A), guanine (G), cytosine (C) and thymine (T) in a DNA molecule</p> Signup and view all the answers

What are the four bases that the DNA sequencing technique aims to determine?

<p>Adenine (A), guanine (G), cytosine (C) and thymine (T)</p> Signup and view all the answers

What does the DNA sequencing technique involve?

<p>Determining the order of the four bases in a DNA molecule</p> Signup and view all the answers

What are the four bases used in DNA sequencing?

<p>adenine (A), guanine (G), cytosine (C), and thymine (T)</p> Signup and view all the answers

What is the purpose of DNA sequencing?

<p>To determine the order of bases in a DNA molecule</p> Signup and view all the answers

How does DNA sequencing help in determining an individual's genetic traits?

<p>By analyzing the sequence of bases in their DNA</p> Signup and view all the answers

Why is DNA sequencing important in the context of the story mentioned?

<p>It determines an individual's genetic suitability for specific roles or opportunities</p> Signup and view all the answers

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