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DNA Sequencing Methods

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Questions and Answers

What temperature is used during the denaturation step of the cycle sequencing reaction?

95°C

How is the clean-up step performed in the cycle sequencing reaction?

By removing non-DNA materials through EtOH/EDTA precipitation

What is the purpose of the cycle sequencing reaction?

To create a population of DNA molecules, each terminating at a different site

What is the purpose of the DNA sequencing technique?

To determine the order of four bases: adenine (A), guanine (G), cytosine (C) and thymine (T) in a DNA molecule Signup and view all the answers

What are the four bases that the DNA sequencing technique aims to determine?

Adenine (A), guanine (G), cytosine (C) and thymine (T) Signup and view all the answers

What does the DNA sequencing technique involve?

Determining the order of the four bases in a DNA molecule Signup and view all the answers

What are the four bases used in DNA sequencing?

adenine (A), guanine (G), cytosine (C), and thymine (T) Signup and view all the answers

What is the purpose of DNA sequencing?

To determine the order of bases in a DNA molecule Signup and view all the answers

How does DNA sequencing help in determining an individual's genetic traits?

By analyzing the sequence of bases in their DNA Signup and view all the answers

Why is DNA sequencing important in the context of the story mentioned?

It determines an individual's genetic suitability for specific roles or opportunities Signup and view all the answers