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DNA Sequencing Techniques Quiz

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Questions and Answers

Which of the following is a key principle of Pyrosequencing?

Detection of DNA fragments through electrophoresis

Detection of pyrophosphate (β-γ phosphates) released during DNA synthesis (correct)

Immobilization and amplification of single-stranded DNA on beads

Reversible termination of DNA synthesis using fluorescent-labeled nucleotides

Which of the following is a characteristic of Next Generation Sequencing (NGS) technologies?

Requires electrophoresis and fragment separation

Produces reads of less than 100 base pairs

Can sequence up to 1 billion base pairs in a single run (correct)

Requires PCR amplification before sequencing

Which of the following NGS technologies uses a semiconductor-based sequencing approach?

Roche/454 Pyrosequencing

SOLiD Enzymatic Method using DNA Ligase

Illumina Reversible Terminator Sequencing

Compact Personal Genome Machine (PGM) Sequencer (correct)

What is a key characteristic of 3rd Generation Sequencing technologies?

Longer read lengths compared to previous NGS technologies Signup and view all the answers

Which of the following is a 3rd Generation Sequencing technology?

HELICOS Single-Molecule Sequencing Device (HeliScope) Signup and view all the answers

What is a key advantage of the Compact Personal Genome Machine (PGM) Sequencer?

Faster turnaround time for sequencing runs Signup and view all the answers

What is the defining feature of the Chain-Termination (Sanger) DNA sequencing method?

Termination of DNA synthesis Signup and view all the answers

Which of the following is NOT a main ingredient used in the Chain-Termination (Sanger) DNA sequencing method?

Radioactive labeling with $^{32}$P Signup and view all the answers

What is the principle behind the Pyrosequencing technique?

Generation of light during nucleotide addition Signup and view all the answers

What is the main advantage of Next-Generation Sequencing (NGS) techniques?

Ability to sequence large numbers of templates simultaneously Signup and view all the answers

What is the key difference between dideoxynucleotides (ddNTPs) and deoxynucleotides (dNTPs)?

ddNTPs lack the 3' OH group, preventing further elongation Signup and view all the answers

Which of the following sequencing methods involves chemical modification of the DNA backbone?

Maxam-Gilbert sequencing Signup and view all the answers