Podcast
Questions and Answers
Which of the following is a key principle of Pyrosequencing?
Which of the following is a key principle of Pyrosequencing?
- Detection of DNA fragments through electrophoresis
- Detection of pyrophosphate (β-γ phosphates) released during DNA synthesis (correct)
- Immobilization and amplification of single-stranded DNA on beads
- Reversible termination of DNA synthesis using fluorescent-labeled nucleotides
Which of the following is a characteristic of Next Generation Sequencing (NGS) technologies?
Which of the following is a characteristic of Next Generation Sequencing (NGS) technologies?
- Requires electrophoresis and fragment separation
- Produces reads of less than 100 base pairs
- Can sequence up to 1 billion base pairs in a single run (correct)
- Requires PCR amplification before sequencing
Which of the following NGS technologies uses a semiconductor-based sequencing approach?
Which of the following NGS technologies uses a semiconductor-based sequencing approach?
- Roche/454 Pyrosequencing
- SOLiD Enzymatic Method using DNA Ligase
- Illumina Reversible Terminator Sequencing
- Compact Personal Genome Machine (PGM) Sequencer (correct)
What is a key characteristic of 3rd Generation Sequencing technologies?
What is a key characteristic of 3rd Generation Sequencing technologies?
Which of the following is a 3rd Generation Sequencing technology?
Which of the following is a 3rd Generation Sequencing technology?
What is a key advantage of the Compact Personal Genome Machine (PGM) Sequencer?
What is a key advantage of the Compact Personal Genome Machine (PGM) Sequencer?
What is the defining feature of the Chain-Termination (Sanger) DNA sequencing method?
What is the defining feature of the Chain-Termination (Sanger) DNA sequencing method?
Which of the following is NOT a main ingredient used in the Chain-Termination (Sanger) DNA sequencing method?
Which of the following is NOT a main ingredient used in the Chain-Termination (Sanger) DNA sequencing method?
What is the principle behind the Pyrosequencing technique?
What is the principle behind the Pyrosequencing technique?
What is the main advantage of Next-Generation Sequencing (NGS) techniques?
What is the main advantage of Next-Generation Sequencing (NGS) techniques?
What is the key difference between dideoxynucleotides (ddNTPs) and deoxynucleotides (dNTPs)?
What is the key difference between dideoxynucleotides (ddNTPs) and deoxynucleotides (dNTPs)?
Which of the following sequencing methods involves chemical modification of the DNA backbone?
Which of the following sequencing methods involves chemical modification of the DNA backbone?
