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Questions and Answers
What is the role of DNA polymerase in replication?
What is the role of DNA polymerase in replication?
- It replicates DNA (correct)
- It synthesizes a short stretch of RNA
- It initiates replication
- It synthesizes a RNA primer
What is the function of Primase in replication?
What is the function of Primase in replication?
- It synthesizes a RNA primer (correct)
- It proofreads DNA
- It replicates DNA
- It initiates replication
What is the length of the RNA primer synthesized by Primase?
What is the length of the RNA primer synthesized by Primase?
- 20 nts long
- 10 nts long (correct)
- 40 nts long
- 30 nts long
Why is RNA polymerase used to synthesize a RNA primer?
Why is RNA polymerase used to synthesize a RNA primer?
What is the role of the RNA primer in DNA replication?
What is the role of the RNA primer in DNA replication?
What happens to the RNA primer after DNA replication is complete?
What happens to the RNA primer after DNA replication is complete?
What is the difference between DNA polymerase and RNA polymerase?
What is the difference between DNA polymerase and RNA polymerase?
What is the function of nucleotides in DNA replication?
What is the function of nucleotides in DNA replication?
What is the function of DNA polymerase in the replication process?
What is the function of DNA polymerase in the replication process?
What is the role of Primase in DNA replication?
What is the role of Primase in DNA replication?
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Study Notes
DNA Replication enzymes and their roles
- DNA Polymerase: Responsible for synthesizing new DNA strands by adding nucleotides to a template strand during DNA replication.
Primase and RNA Primer
- Primase: An enzyme that synthesizes short RNA primers, which are complementary to the template DNA strand, providing a starting point for DNA synthesis.
- Length of RNA primer: Typically 10-12 nucleotides long.
- RNA primer function: The RNA primer provides a 3' hydroxyl group for DNA synthesis to initiate, and acts as a starting point for DNA polymerase to begin synthesizing new DNA.
RNA Polymerase vs. DNA Polymerase
- RNA polymerase: Not used to synthesize RNA primers in DNA replication; instead, Primase is responsible for this.
- Difference between DNA polymerase and RNA polymerase: RNA polymerase is used for transcription, synthesizing RNA from a DNA template, whereas DNA polymerase is used for DNA replication, synthesizing new DNA strands from a template strand.
Nucleotides and DNA Replication
- Nucleotides function: Building blocks of DNA, providing the raw material for DNA synthesis during replication.
Post-Replication
- RNA primer removal: After DNA replication is complete, the RNA primer is removed and replaced with DNA nucleotides, ensuring a continuous DNA strand.
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