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Questions and Answers
What is the function of RNA primers in lagging strand synthesis?
What is the function of RNA primers in lagging strand synthesis?
- To break the replication fork
- To stabilize the DNA molecule
- To provide a starting point for DNA polymerase III (correct)
- To synthesize DNA fragments
Why does DNA polymerase III need many RNA primers in lagging strand synthesis?
Why does DNA polymerase III need many RNA primers in lagging strand synthesis?
- To stabilize the DNA molecule
- To synthesize DNA fragments
- To provide a starting point for DNA polymerase III (correct)
- To break the replication fork
What is the size of the short DNA fragments synthesized by DNA polymerase III in lagging strand synthesis?
What is the size of the short DNA fragments synthesized by DNA polymerase III in lagging strand synthesis?
- 100 nts
- 500 nts
- 1000 nts (correct)
- 2000 nts
What is the name given to the short DNA fragments synthesized in the lagging strand synthesis?
What is the name given to the short DNA fragments synthesized in the lagging strand synthesis?
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What is the role of helicase in the lagging strand synthesis?
What is the role of helicase in the lagging strand synthesis?
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Study Notes
Lagging Strand Synthesis
- RNA primers provide a starting point for DNA synthesis in the lagging strand, allowing DNA polymerase III to synthesize short DNA fragments.
Function of RNA Primers
- Multiple RNA primers are needed in lagging strand synthesis because DNA polymerase III synthesizes short DNA fragments in a discontinuous manner.
Characteristics of Short DNA Fragments
- The short DNA fragments synthesized by DNA polymerase III in lagging strand synthesis are around 1000-2000 nucleotides in length.
Naming of Short DNA Fragments
- The short DNA fragments synthesized in lagging strand synthesis are called Okazaki fragments.
Role of Helicase
- Helicase unwinds the double-stranded DNA, creating a replication fork that allows access to the lagging strand, where synthesis can occur.
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