DNA Replication Process
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Questions and Answers

What is the function of RNase H during DNA replication?

  • To degrade RNA primers and replace them with DNA nucleotides (correct)
  • To synthesize RNA primers
  • To unwind DNA and break H-bonds
  • To connect Okazaki fragments to one another

During the elongation step of DNA replication, which enzyme is responsible for synthesizing the matching strand in the 5’ to 3’ direction?

  • DNA polymerase (correct)
  • RNase H
  • DNA primase
  • Helicase

During the termination step of DNA replication, what is the role of the protein that binds to the termination site sequence?

  • To connect Okazaki fragments to one another
  • To unwind DNA and break H-bonds
  • To physically stop DNA replication (correct)
  • To synthesize the matching strand in the 5’ to 3’ direction

What is the function of DNA primase during DNA replication?

<p>To create RNA primers (C)</p> Signup and view all the answers

What is the characteristic of Okazaki fragments synthesized during DNA replication?

<p>Short, discontinuous fragments (C)</p> Signup and view all the answers

What is the purpose of the RNA primer during DNA replication?

<p>To provide a free 3’-OH group for DNA synthesis (A)</p> Signup and view all the answers

What is the role of sigma factor in RNA synthesis in E. coli?

<p>To recognize the promoter of a gene (C)</p> Signup and view all the answers

What is the function of the TATA-binding protein (TBP) in eukaryotic cells?

<p>To recognize the TATA box (D)</p> Signup and view all the answers

What happens when RNA polymerase encounters a terminator sequence in E. coli?

<p>It releases the DNA template and the RNA transcript (D)</p> Signup and view all the answers

What is the role of general transcription factors in eukaryotic cells?

<p>To assemble at the promoter (D)</p> Signup and view all the answers

What is the first step in messenger RNA synthesis in both E. coli and eukaryotes?

<p>Initiation (B)</p> Signup and view all the answers

What is the result of the termination step in E. coli?

<p>The RNA polymerase releases the DNA template and the RNA transcript (B)</p> Signup and view all the answers

What is the primary function of RNA primer in DNA replication?

<p>To serve as a starting point for DNA synthesis (A)</p> Signup and view all the answers

What type of RNA is involved in the initiation step of DNA replication?

<p>RNA primer (D)</p> Signup and view all the answers

During DNA replication, what is the function of Helicase?

<p>To unwind the DNA double helix by breaking hydrogen bonds (A)</p> Signup and view all the answers

What is the process called when old and new DNA strands are mixed during DNA replication?

<p>Semi-conservative replication (C)</p> Signup and view all the answers

What is the name of the enzyme that prevents the DNA double helix from re-annealing after DNA helicase unwinds?

<p>Single-Strand Binding (SSB) Proteins (D)</p> Signup and view all the answers

What is the name of the point on the DNA molecule where the replication process starts?

<p>Origin of Replication (C)</p> Signup and view all the answers

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