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DNA Replication Process

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Questions and Answers

What is the function of RNase H during DNA replication?

To degrade RNA primers and replace them with DNA nucleotides (correct)

To synthesize RNA primers

To unwind DNA and break H-bonds

To connect Okazaki fragments to one another

During the elongation step of DNA replication, which enzyme is responsible for synthesizing the matching strand in the 5’ to 3’ direction?

DNA polymerase (correct)

RNase H

DNA primase

Helicase

During the termination step of DNA replication, what is the role of the protein that binds to the termination site sequence?

To connect Okazaki fragments to one another

To unwind DNA and break H-bonds

To physically stop DNA replication (correct)

To synthesize the matching strand in the 5’ to 3’ direction

What is the function of DNA primase during DNA replication?

To create RNA primers Signup and view all the answers

What is the characteristic of Okazaki fragments synthesized during DNA replication?

Short, discontinuous fragments Signup and view all the answers

What is the purpose of the RNA primer during DNA replication?

To provide a free 3’-OH group for DNA synthesis Signup and view all the answers

What is the role of sigma factor in RNA synthesis in E. coli?

To recognize the promoter of a gene Signup and view all the answers

What is the function of the TATA-binding protein (TBP) in eukaryotic cells?

To recognize the TATA box Signup and view all the answers

What happens when RNA polymerase encounters a terminator sequence in E. coli?

It releases the DNA template and the RNA transcript Signup and view all the answers

What is the role of general transcription factors in eukaryotic cells?

To assemble at the promoter Signup and view all the answers

What is the first step in messenger RNA synthesis in both E. coli and eukaryotes?

Initiation Signup and view all the answers

What is the result of the termination step in E. coli?

The RNA polymerase releases the DNA template and the RNA transcript Signup and view all the answers

What is the primary function of RNA primer in DNA replication?

To serve as a starting point for DNA synthesis Signup and view all the answers

What type of RNA is involved in the initiation step of DNA replication?

RNA primer Signup and view all the answers

During DNA replication, what is the function of Helicase?

To unwind the DNA double helix by breaking hydrogen bonds Signup and view all the answers

What is the process called when old and new DNA strands are mixed during DNA replication?

Semi-conservative replication Signup and view all the answers

What is the name of the enzyme that prevents the DNA double helix from re-annealing after DNA helicase unwinds?

Single-Strand Binding (SSB) Proteins Signup and view all the answers

What is the name of the point on the DNA molecule where the replication process starts?

Origin of Replication Signup and view all the answers