DNA Replication Overview
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Questions and Answers

What is the main function of tRNA during cell division and transformation?

  • Synthesizing proteins
  • Transporting daughter cells
  • Reading of mRNA by ribosomes (correct)
  • Splicing in eukaryotes
  • How long are transfer RNA (tRNA) molecules on average?

  • 50-60 bases
  • 73-93 bases (correct)
  • 100-120 bases
  • 10-20 bases
  • Where is small nuclear RNA (snRNA) primarily located after transcription?

  • Endoplasmic reticulum
  • Mitochondria
  • Cytoplasm
  • Nucleus (correct)
  • Which RNA polymerase is responsible for the synthesis of mRNA?

    <p>RNA polymerase II</p> Signup and view all the answers

    Where does transcription primarily take place in prokaryotes?

    <p>Cytoplasm</p> Signup and view all the answers

    What determines the termination of RNA synthesis in prokaryotes?

    <p>High levels of gene product</p> Signup and view all the answers

    What is the direction in which DNA polymerase reads the template strand during DNA replication?

    <p>3' to 5'</p> Signup and view all the answers

    What is the key to maintaining the sequence of nucleotides in DNA through new generations?

    <p>Semi-conservative replication</p> Signup and view all the answers

    What is the purpose of the replication apparatus during cell division?

    <p>All of the above</p> Signup and view all the answers

    What is the relationship between the two strands of the DNA double helix?

    <p>Complementary and antiparallel</p> Signup and view all the answers

    What is the purpose of the hydrogen bonds formed between the two complementary strands of DNA?

    <p>To stabilize the DNA double helix structure</p> Signup and view all the answers

    Why is it important that the DNA sequence information be transferred faithfully during cell division?

    <p>All of the above</p> Signup and view all the answers

    Which of the following statements is correct about eukaryotic ribosomal RNA (rRNA)?

    <p>The 5S rRNA is transcribed separately from the 45S precursor RNA.</p> Signup and view all the answers

    What is the primary function of messenger RNA (mRNA) in both prokaryotes and eukaryotes?

    <p>To carry genetic information from DNA to the translation machinery for protein synthesis.</p> Signup and view all the answers

    Which of the following statements is true about prokaryotic mRNA?

    <p>It is synthesized and translated simultaneously.</p> Signup and view all the answers

    What is the primary function of plasmids in prokaryotic cells?

    <p>To provide additional genetic information beyond the main chromosome.</p> Signup and view all the answers

    Which of the following statements accurately describes large plasmids?

    <p>They carry genes for their own transfer and propagation, and occur in low copy numbers.</p> Signup and view all the answers

    What is the primary difference between large and small plasmids in prokaryotic cells?

    <p>Large plasmids carry genes for their own maintenance, while small plasmids do not.</p> Signup and view all the answers

    What is the main function of DNA polymerase I (pol I)?

    <p>It has a large subunit called the Klenow fragment that is used for in vitro DNA synthesis.</p> Signup and view all the answers

    What is the key difference between sexual and asexual recombination?

    <p>Sexual recombination involves the pairing and crossing over of DNA duplexes during meiosis, while asexual recombination involves the transfer of genetic information between bacteria through conjugation.</p> Signup and view all the answers

    What is the role of the F factor in bacterial conjugation?

    <p>The F factor is an extrachromosomal circle of double-stranded DNA or plasmid that carries the genes coding for the construction of the mating bridge during conjugation.</p> Signup and view all the answers

    What is the purpose of recombination in DNA?

    <p>Recombination is the process by which new genetic combinations are created through the mixture and assembly of existing DNA sequences.</p> Signup and view all the answers

    What is the significance of the recombination process during meiosis?

    <p>Recombination during meiosis creates genetic diversity by randomly assorting the recombined chromosomes into the gametes.</p> Signup and view all the answers

    What is the key difference between F+ and F- bacteria in the context of bacterial conjugation?

    <p>F+ bacteria can transfer genetic information to F- bacteria, but F- bacteria cannot transfer genetic information to F+ bacteria.</p> Signup and view all the answers

    What is the primary role of RNA polymerase during transcription?

    <p>To copy the DNA template with complementary bases</p> Signup and view all the answers

    Which of the following enzymes is responsible for cleaving double-stranded RNA?

    <p>RNase III</p> Signup and view all the answers

    What is the primary function of RNase P?

    <p>To remove precursor nucleotides from tRNA molecules</p> Signup and view all the answers

    Which of the following is a characteristic of RNA helicases?

    <p>They catalyze the unwinding of double-stranded RNA</p> Signup and view all the answers

    What is the primary difference between endoribonucleases and exoribonucleases?

    <p>Endoribonucleases degrade the internal part of RNA, while exoribonucleases degrade RNA from the 3' or 5' ends</p> Signup and view all the answers

    Which of the following enzymes is responsible for digesting the RNA strand in a DNA-RNA hybrid?

    <p>RNase H</p> Signup and view all the answers

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