DNA Replication Overview

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Questions and Answers

What is the primary purpose of DNA replication?

  • To ensure new cells receive exact copies of the DNA (correct)
  • To generate energy for the cell
  • To repair damaged DNA
  • To create proteins for the cell

DNA replication is essential only during organism growth, not during tissue repair.

False (B)

What is meant by the term 'semi-conservative' in DNA replication?

It means each new DNA double helix contains one original strand and one newly synthesized strand.

During DNA replication, Adenine pairs with _____ and Cytosine pairs with _____.

<p>Thymine, Guanine</p> Signup and view all the answers

Match the following stages of life processes with their descriptions:

<p>Growth = Adding new cells Tissue Repair = Replacing damaged cells Cell Differentiation = Specialization of cells Cell Division = Process of replicating DNA for new cells</p> Signup and view all the answers

How long does it typically take for DNA replication to occur in human cells?

<p>One hour (C)</p> Signup and view all the answers

Fast DNA replication ensures trillions of identical cells can develop from one fertilized egg.

<p>True (A)</p> Signup and view all the answers

What mnemonic can help remember the processes that depend on DNA replication?

<p>&quot;GROWTH Gap Repair&quot;</p> Signup and view all the answers

What term describes the phenomenon where multiple codons code for the same amino acid?

<p>Degeneracy (C)</p> Signup and view all the answers

The sequence of DNA varies significantly among all organisms.

<p>False (B)</p> Signup and view all the answers

What is the function of haemoglobin in red blood cells?

<p>To transport oxygen and carbon dioxide.</p> Signup and view all the answers

The __________ is the site in the ribosome where tRNA first enters carrying its amino acid.

<p>A site</p> Signup and view all the answers

How many nucleotides are required to code for 240 amino acids?

<p>720 nucleotides (C)</p> Signup and view all the answers

Match the following ribosome sites with their functions:

<p>A site = Holds the incoming tRNA with new amino acid P site = Where peptide bond formation occurs E site = Exit site for tRNA after amino acid transfer</p> Signup and view all the answers

Conserved sequences are those that change rapidly across generations.

<p>False (B)</p> Signup and view all the answers

Which proteins, besides haemoglobin, are not required in red blood cells?

<p>Actin, myosin, immunoglobulins, lactase.</p> Signup and view all the answers

What is the primary purpose of gel electrophoresis in DNA profiling?

<p>To separate DNA fragments by size (B)</p> Signup and view all the answers

Larger DNA fragments move farther in gel electrophoresis compared to smaller fragments.

<p>False (B)</p> Signup and view all the answers

What method is used to amplify DNA from minute samples?

<p>PCR (Polymerase Chain Reaction)</p> Signup and view all the answers

The number of times a tandem repeat is repeated can vary widely between __________.

<p>individuals</p> Signup and view all the answers

Match the following types of tandem repeats with their lengths:

<p>Microsatellites = 1-6 base pairs Minisatellites = 10-60 base pairs Macrosatellites = Larger than 100 base pairs</p> Signup and view all the answers

What is the function of a DNA ladder in gel electrophoresis?

<p>To provide a reference for estimating fragment sizes (B)</p> Signup and view all the answers

Restriction enzymes are used to amplify DNA fragments for analysis.

<p>False (B)</p> Signup and view all the answers

In DNA profiling, what is compared to determine biological relationships?

<p>DNA fragments from the child, mother, and alleged father</p> Signup and view all the answers

During __________, DNA moves toward the positive electrode due to its negative charge.

<p>electrophoresis</p> Signup and view all the answers

Match the following terms with their descriptions:

<p>PCR = Amplifies DNA samples Gel electrophoresis = Separates DNA by size Restriction enzymes = Cut DNA at specific sites</p> Signup and view all the answers

Why are tandem repeats useful in forensic investigations?

<p>They exhibit a high degree of variability between individuals. (D)</p> Signup and view all the answers

Ethidium bromide is used to visualize DNA bands under infrared light.

<p>False (B)</p> Signup and view all the answers

What role do restriction endonucleases play in DNA profiling?

<p>They cut DNA into smaller fragments at specific sequences.</p> Signup and view all the answers

The process of comparing the distance traveled by sample fragments to a DNA ladder is part of determining the __________ of DNA fragments.

<p>size</p> Signup and view all the answers

What is the main function of helicase in DNA replication?

<p>Unwinds the DNA double helix (A)</p> Signup and view all the answers

DNA polymerase can initiate DNA synthesis without a primer.

<p>False (B)</p> Signup and view all the answers

What base pairs with adenine (A) in DNA?

<p>thymine (T)</p> Signup and view all the answers

The _____ enzyme synthesizes new DNA strands using the template.

<p>DNA polymerase</p> Signup and view all the answers

Match the following phases of PCR with their descriptions:

<p>Denaturation = Separates DNA into single strands Annealing = Primers bind to the template Extension = Taq polymerase extends the DNA strand</p> Signup and view all the answers

Which phase of PCR is characterized by the temperature being raised to 98°C?

<p>Denaturation Phase (C)</p> Signup and view all the answers

PCR can amplify DNA from minute samples to millions of copies.

<p>True (A)</p> Signup and view all the answers

What is the purpose of gel electrophoresis in PCR?

<p>To visualize and analyze amplified DNA fragments.</p> Signup and view all the answers

In eukaryotic DNA, the first step of replication involves the _____ of the double helix.

<p>unwinding</p> Signup and view all the answers

Match the following enzymes with their roles:

<p>Helicase = Unwinds DNA DNA Polymerase = Synthesizes new strands Taq Polymerase = Extends DNA at high temperature</p> Signup and view all the answers

What does the acronym STR stand for in DNA profiling?

<p>Short Tandem Repeats (A)</p> Signup and view all the answers

In the PCR process, the extension phase uses Taq polymerase, which is heat-stable.

<p>True (A)</p> Signup and view all the answers

What is the significance of proof-reading in DNA polymerase?

<p>To correct mismatched bases and ensure replication accuracy.</p> Signup and view all the answers

Each cycle of PCR results in _____ the amount of DNA.

<p>doubling</p> Signup and view all the answers

What role do primers play in PCR?

<p>They bind to their complementary sequences (D)</p> Signup and view all the answers

What is the main function of RNA polymerase during transcription?

<p>To unwind the DNA double helix (B)</p> Signup and view all the answers

Transcription occurs in the cytoplasm in eukaryotic cells.

<p>False (B)</p> Signup and view all the answers

What is the role of mRNA in protein synthesis?

<p>To deliver genetic information from the nucleus to the ribosomes for translation.</p> Signup and view all the answers

The start codon on mRNA is always _____ and it codes for _____ as the first amino acid.

<p>AUG; methionine</p> Signup and view all the answers

Match the RNA structures to their functions:

<p>mRNA = Carries genetic information to ribosomes tRNA = Brings amino acids to ribosome rRNA = Forms part of the ribosome structure RNA polymerase = Synthesizes RNA from the DNA template</p> Signup and view all the answers

What distinguishes RNA from DNA?

<p>RNA contains uracil while DNA contains thymine (C)</p> Signup and view all the answers

Transcription can occur simultaneously on both strands of DNA.

<p>False (B)</p> Signup and view all the answers

How many nucleotides are needed in the coding strand to produce a polypeptide of 240 amino acids?

<p>720 nucleotides</p> Signup and view all the answers

The _____ gene may only be switched on during the summer.

<p>melanin</p> Signup and view all the answers

Match the following terms with their correct definitions:

<p>Codon = A sequence of three nucleotides on mRNA Polypeptide = A chain of amino acids Gene expression = The process of turning genes on or off Transcriptome = The full range of RNA types expressed in a cell</p> Signup and view all the answers

During translation, which site on the ribosome is responsible for peptide bond formation?

<p>P site (A)</p> Signup and view all the answers

A single mRNA molecule can only be translated once.

<p>False (B)</p> Signup and view all the answers

What happens to the DNA after RNA polymerase leaves during transcription?

<p>The DNA rewinds back into a double helix.</p> Signup and view all the answers

The _____ strand of DNA serves as the template during transcription.

<p>anti-sense</p> Signup and view all the answers

What is the primary purpose of the ribosome in translation?

<p>To facilitate the assembly of polypeptides (D)</p> Signup and view all the answers

Flashcards

DNA replication

The process of copying DNA for cell division.

Cell division

The process that results in two or more daughter cells from a parent cell.

Importance of accurate DNA copies

Ensures new cells inherit correct genetic information.

Speed of DNA replication

Can duplicate billions of base pairs in about one hour.

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Semi-conservative replication

Each new DNA molecule has one old and one new strand.

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Template strand

Parent DNA strand used to create a new complementary strand.

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Complementary base pairing

A pairs with T, C pairs with G during replication.

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Result of DNA replication

Two identical DNA molecules ensuring genetic continuity.

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Role of Helicase

Helicase unwinds the DNA double helix by breaking hydrogen bonds between base pairs.

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Role of DNA Polymerase

DNA polymerase synthesizes new DNA strands by pairing complementary nucleotides.

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Base Pairing Rule

Adenine (A) pairs with Thymine (T), and Cytosine (C) pairs with Guanine (G).

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PCR (Polymerase Chain Reaction)

Technique to amplify specific DNA sequences, producing millions of copies from small samples.

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Denaturation Phase

First step of PCR where DNA is heated to separate into single strands.

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Annealing Phase

Second step of PCR where temperature lowers, enabling primers to bind to DNA.

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Extension Phase

Third step in PCR where Taq polymerase adds nucleotides to synthesize new DNA strands.

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Taq Polymerase

Heat-stable enzyme used in the extension phase of PCR for adding nucleotides.

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Exponential Growth in PCR

Each cycle of PCR doubles the amount of DNA, leading to billions of copies after multiple cycles.

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Gel Electrophoresis

Technique used to separate DNA fragments by size after PCR amplification.

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DNA Profiling

Analyzing variable DNA regions to create a unique genetic fingerprint for individuals.

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Applications of PCR

PCR can verify results, analyze genetic variations, and isolate DNA fragments for study.

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Applications in Forensics

Using DNA analysis from crime scenes for investigations, often via PCR and gel electrophoresis.

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Overview of DNA Replication Enzymes

Helicase unwinds DNA; DNA polymerase synthesizes new strands ensuring accurate base pairing.

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Triplet Code

A genetic code comprising three nucleotides that specifies an amino acid.

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Degenerate Code

Multiple codons can encode the same amino acid, providing genetic redundancy.

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tRNA Function

tRNA transports amino acids to the ribosome and helps form peptide bonds.

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Ribosome Sites

The ribosome has three sites: A site (aminoacyl), P site (peptidyl), E site (exit).

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Peptide Bond Formation

The chemical bond formed between amino acids during protein synthesis.

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Universal DNA

The genetic structure of DNA is consistent across all living organisms.

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Conserved DNA

DNA sequences that remain unchanged over generations due to essential functions.

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Haemoglobin Role

Hemoglobin is the protein in red blood cells that transports oxygen.

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Transcription

The synthesis of RNA using DNA as a template, occurring in the nucleus.

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RNA polymerase

An enzyme that synthesizes RNA by binding to DNA and unwinding it.

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Coding strand

The DNA strand that matches the RNA sequence except for T and U.

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Transcriptomes

The complete set of RNA transcripts produced by a cell.

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Translation

The synthesis of polypeptides from mRNA in the ribosomes.

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Codons

Sequences of three nucleotides on mRNA that specify amino acids.

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Start codon

The first codon in mRNA (AUG) that signals the beginning of translation.

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Stop codon

The codon that signals the end of translation.

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Ribosomal complex

The structure made of ribosomal subunits where translation occurs.

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tRNA

Transfer RNA that carries amino acids to the ribosome during translation.

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Amino acids

The building blocks of proteins, linked in specific sequences.

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Nucleotide requirement

To code for a polypeptide, 3 nucleotides are needed for each amino acid.

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Gene expression regulation

Not all genes are active at all times; some can be switched on or off.

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DNA Identification

Using DNA profiles to identify victims by matching samples.

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Trace Evidence

Small biological materials like hair amplified for identification.

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Paternity Testing

Comparing DNA from child, mother, and alleged father to establish relationships.

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PCR Amplification

Process that increases DNA quantity from small samples.

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DNA Patterns

Unique arrangements of DNA fragments visualized after separation.

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DNA Ladder

Reference mix of DNA fragments used for measuring sizes.

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Electric Current in Gel

Drives DNA migration during gel electrophoresis.

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Restriction Enzymes

Enzymes that cut DNA into smaller fragments.

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Tandem Repeats

Short sequences of DNA repeated in the genome, varying by individual.

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Microsatellites

Short tandem repeats of 1-6 base pairs in DNA.

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Minisatellites

Repeat sequences of 10-60 base pairs.

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Macrosatellites

Long tandem repeats of greater than 100 base pairs.

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Visualization in Gel

Using dyes to make DNA bands visible under UV light.

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Size-Based Separation

Smaller DNA fragments move faster in the gel than larger ones.

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Study Notes

DNA Replication Overview

  • DNA replication is crucial for cell division (growth and repair).
  • Ensures new cells inherit the correct genetic information for proper function.
  • Happens at a high speed (approximately one hour in human cells) while maintaining accuracy.
  • Allows for multicellular organism development from a single cell.
  • Essential for proper cell differentiation during repair.

Semi-Conservative Replication

  • DNA replication is semi-conservative.
  • Each new DNA molecule has one original (parent) strand and one newly synthesized strand.
  • Parental strands act as templates.
  • Complementary base pairing (A-T, C-G) ensures accuracy.
  • Results in two identical DNA molecules.

Helicase and DNA Polymerase in Replication

  • Helicase unwinds the DNA double helix by breaking hydrogen bonds between bases.
  • This action exposes the template strands for replication.
  • DNA Polymerase builds new strands using free nucleotides and follows the 5' to 3' direction, matching bases (A-T, C-G).
  • DNA polymerase has a proofreading mechanism to ensure accuracy.

Polymerase Chain Reaction (PCR)

  • PCR amplifies specific DNA sequences.
  • Key Steps: Denaturation, Annealing, Extension.
  • Denaturation (98°C): Separates DNA strands using heat.
  • Annealing (60°C): Allows primers to bind to specific DNA targets.
  • Extension (72°C): Taq polymerase adds nucleotides to extend strands.
  • Each cycle doubles the DNA amount, resulting in exponential growth.
  • Essential for sufficient DNA in further analyses (e.g., gel electrophoresis).

Gel Electrophoresis

  • Separates DNA fragments by size.
  • Smaller fragments migrate further through the gel than larger fragments.
  • DNA ladders (known fragment lengths) used to determine sample fragment sizes.
  • Crucial for visualizing DNA profiles and confirming PCR success.
  • Crucial in applications of DNA profiling.

Tandem Repeats in DNA Profiling

  • Tandem repeats (short, repeated sequences) show variation between individuals.
  • Restriction enzymes cut DNA at specific locations creating fragments of different sizes.
  • PCR amplifies the fragments.
  • Separation occurs via Gel Electrophoresis.
  • Useful for forensic investigations and paternity testing.

Transcription

  • Synthesizes RNA from DNA template.
  • Occurs in the nucleus.
  • Uses one DNA strand as a template.
  • RNA polymerase unwinds DNA and brings RNA nucleotides for complementary pairing.
  • Creates a new RNA strand complementary to the template.
  • Replicated parts of DNA can be copied many times until needed.

Translation

  • Synthesis of polypeptides (proteins).
  • Uses mRNA as a guide.
  • Codons (3-nucleotide sequences) on mRNA specify amino acid order.
  • Ribosomes assemble polypeptides.
  • tRNAs deliver amino acids.
  • Start codon (AUG) begins translation and codes for methionine (Met).
  • Stop codons end translation.

Degeneracy and Conserved DNA

  • Degeneracy: Multiple codons can code for the same amino acid.
  • Conserved DNA sequences remain unchanged over time due to their essential functions.

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