DNA Replication Overview
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Questions and Answers

What is the primary purpose of DNA replication?

  • To ensure new cells receive exact copies of the DNA (correct)
  • To generate energy for the cell
  • To repair damaged DNA
  • To create proteins for the cell
  • DNA replication is essential only during organism growth, not during tissue repair.

    False (B)

    What is meant by the term 'semi-conservative' in DNA replication?

    It means each new DNA double helix contains one original strand and one newly synthesized strand.

    During DNA replication, Adenine pairs with _____ and Cytosine pairs with _____.

    <p>Thymine, Guanine</p> Signup and view all the answers

    Match the following stages of life processes with their descriptions:

    <p>Growth = Adding new cells Tissue Repair = Replacing damaged cells Cell Differentiation = Specialization of cells Cell Division = Process of replicating DNA for new cells</p> Signup and view all the answers

    How long does it typically take for DNA replication to occur in human cells?

    <p>One hour (C)</p> Signup and view all the answers

    Fast DNA replication ensures trillions of identical cells can develop from one fertilized egg.

    <p>True (A)</p> Signup and view all the answers

    What mnemonic can help remember the processes that depend on DNA replication?

    <p>&quot;GROWTH Gap Repair&quot;</p> Signup and view all the answers

    What term describes the phenomenon where multiple codons code for the same amino acid?

    <p>Degeneracy (C)</p> Signup and view all the answers

    The sequence of DNA varies significantly among all organisms.

    <p>False (B)</p> Signup and view all the answers

    What is the function of haemoglobin in red blood cells?

    <p>To transport oxygen and carbon dioxide.</p> Signup and view all the answers

    The __________ is the site in the ribosome where tRNA first enters carrying its amino acid.

    <p>A site</p> Signup and view all the answers

    How many nucleotides are required to code for 240 amino acids?

    <p>720 nucleotides (C)</p> Signup and view all the answers

    Match the following ribosome sites with their functions:

    <p>A site = Holds the incoming tRNA with new amino acid P site = Where peptide bond formation occurs E site = Exit site for tRNA after amino acid transfer</p> Signup and view all the answers

    Conserved sequences are those that change rapidly across generations.

    <p>False (B)</p> Signup and view all the answers

    Which proteins, besides haemoglobin, are not required in red blood cells?

    <p>Actin, myosin, immunoglobulins, lactase.</p> Signup and view all the answers

    What is the primary purpose of gel electrophoresis in DNA profiling?

    <p>To separate DNA fragments by size (B)</p> Signup and view all the answers

    Larger DNA fragments move farther in gel electrophoresis compared to smaller fragments.

    <p>False (B)</p> Signup and view all the answers

    What method is used to amplify DNA from minute samples?

    <p>PCR (Polymerase Chain Reaction)</p> Signup and view all the answers

    The number of times a tandem repeat is repeated can vary widely between __________.

    <p>individuals</p> Signup and view all the answers

    Match the following types of tandem repeats with their lengths:

    <p>Microsatellites = 1-6 base pairs Minisatellites = 10-60 base pairs Macrosatellites = Larger than 100 base pairs</p> Signup and view all the answers

    What is the function of a DNA ladder in gel electrophoresis?

    <p>To provide a reference for estimating fragment sizes (B)</p> Signup and view all the answers

    Restriction enzymes are used to amplify DNA fragments for analysis.

    <p>False (B)</p> Signup and view all the answers

    In DNA profiling, what is compared to determine biological relationships?

    <p>DNA fragments from the child, mother, and alleged father</p> Signup and view all the answers

    During __________, DNA moves toward the positive electrode due to its negative charge.

    <p>electrophoresis</p> Signup and view all the answers

    Match the following terms with their descriptions:

    <p>PCR = Amplifies DNA samples Gel electrophoresis = Separates DNA by size Restriction enzymes = Cut DNA at specific sites</p> Signup and view all the answers

    Why are tandem repeats useful in forensic investigations?

    <p>They exhibit a high degree of variability between individuals. (D)</p> Signup and view all the answers

    Ethidium bromide is used to visualize DNA bands under infrared light.

    <p>False (B)</p> Signup and view all the answers

    What role do restriction endonucleases play in DNA profiling?

    <p>They cut DNA into smaller fragments at specific sequences.</p> Signup and view all the answers

    The process of comparing the distance traveled by sample fragments to a DNA ladder is part of determining the __________ of DNA fragments.

    <p>size</p> Signup and view all the answers

    What is the main function of helicase in DNA replication?

    <p>Unwinds the DNA double helix (A)</p> Signup and view all the answers

    DNA polymerase can initiate DNA synthesis without a primer.

    <p>False (B)</p> Signup and view all the answers

    What base pairs with adenine (A) in DNA?

    <p>thymine (T)</p> Signup and view all the answers

    The _____ enzyme synthesizes new DNA strands using the template.

    <p>DNA polymerase</p> Signup and view all the answers

    Match the following phases of PCR with their descriptions:

    <p>Denaturation = Separates DNA into single strands Annealing = Primers bind to the template Extension = Taq polymerase extends the DNA strand</p> Signup and view all the answers

    Which phase of PCR is characterized by the temperature being raised to 98°C?

    <p>Denaturation Phase (C)</p> Signup and view all the answers

    PCR can amplify DNA from minute samples to millions of copies.

    <p>True (A)</p> Signup and view all the answers

    What is the purpose of gel electrophoresis in PCR?

    <p>To visualize and analyze amplified DNA fragments.</p> Signup and view all the answers

    In eukaryotic DNA, the first step of replication involves the _____ of the double helix.

    <p>unwinding</p> Signup and view all the answers

    Match the following enzymes with their roles:

    <p>Helicase = Unwinds DNA DNA Polymerase = Synthesizes new strands Taq Polymerase = Extends DNA at high temperature</p> Signup and view all the answers

    What does the acronym STR stand for in DNA profiling?

    <p>Short Tandem Repeats (A)</p> Signup and view all the answers

    In the PCR process, the extension phase uses Taq polymerase, which is heat-stable.

    <p>True (A)</p> Signup and view all the answers

    What is the significance of proof-reading in DNA polymerase?

    <p>To correct mismatched bases and ensure replication accuracy.</p> Signup and view all the answers

    Each cycle of PCR results in _____ the amount of DNA.

    <p>doubling</p> Signup and view all the answers

    What role do primers play in PCR?

    <p>They bind to their complementary sequences (D)</p> Signup and view all the answers

    What is the main function of RNA polymerase during transcription?

    <p>To unwind the DNA double helix (B)</p> Signup and view all the answers

    Transcription occurs in the cytoplasm in eukaryotic cells.

    <p>False (B)</p> Signup and view all the answers

    What is the role of mRNA in protein synthesis?

    <p>To deliver genetic information from the nucleus to the ribosomes for translation.</p> Signup and view all the answers

    The start codon on mRNA is always _____ and it codes for _____ as the first amino acid.

    <p>AUG; methionine</p> Signup and view all the answers

    Match the RNA structures to their functions:

    <p>mRNA = Carries genetic information to ribosomes tRNA = Brings amino acids to ribosome rRNA = Forms part of the ribosome structure RNA polymerase = Synthesizes RNA from the DNA template</p> Signup and view all the answers

    What distinguishes RNA from DNA?

    <p>RNA contains uracil while DNA contains thymine (C)</p> Signup and view all the answers

    Transcription can occur simultaneously on both strands of DNA.

    <p>False (B)</p> Signup and view all the answers

    How many nucleotides are needed in the coding strand to produce a polypeptide of 240 amino acids?

    <p>720 nucleotides</p> Signup and view all the answers

    The _____ gene may only be switched on during the summer.

    <p>melanin</p> Signup and view all the answers

    Match the following terms with their correct definitions:

    <p>Codon = A sequence of three nucleotides on mRNA Polypeptide = A chain of amino acids Gene expression = The process of turning genes on or off Transcriptome = The full range of RNA types expressed in a cell</p> Signup and view all the answers

    During translation, which site on the ribosome is responsible for peptide bond formation?

    <p>P site (A)</p> Signup and view all the answers

    A single mRNA molecule can only be translated once.

    <p>False (B)</p> Signup and view all the answers

    What happens to the DNA after RNA polymerase leaves during transcription?

    <p>The DNA rewinds back into a double helix.</p> Signup and view all the answers

    The _____ strand of DNA serves as the template during transcription.

    <p>anti-sense</p> Signup and view all the answers

    What is the primary purpose of the ribosome in translation?

    <p>To facilitate the assembly of polypeptides (D)</p> Signup and view all the answers

    Flashcards

    DNA replication

    The process of copying DNA for cell division.

    Cell division

    The process that results in two or more daughter cells from a parent cell.

    Importance of accurate DNA copies

    Ensures new cells inherit correct genetic information.

    Speed of DNA replication

    Can duplicate billions of base pairs in about one hour.

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    Semi-conservative replication

    Each new DNA molecule has one old and one new strand.

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    Template strand

    Parent DNA strand used to create a new complementary strand.

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    Complementary base pairing

    A pairs with T, C pairs with G during replication.

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    Result of DNA replication

    Two identical DNA molecules ensuring genetic continuity.

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    Role of Helicase

    Helicase unwinds the DNA double helix by breaking hydrogen bonds between base pairs.

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    Role of DNA Polymerase

    DNA polymerase synthesizes new DNA strands by pairing complementary nucleotides.

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    Base Pairing Rule

    Adenine (A) pairs with Thymine (T), and Cytosine (C) pairs with Guanine (G).

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    PCR (Polymerase Chain Reaction)

    Technique to amplify specific DNA sequences, producing millions of copies from small samples.

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    Denaturation Phase

    First step of PCR where DNA is heated to separate into single strands.

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    Annealing Phase

    Second step of PCR where temperature lowers, enabling primers to bind to DNA.

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    Extension Phase

    Third step in PCR where Taq polymerase adds nucleotides to synthesize new DNA strands.

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    Taq Polymerase

    Heat-stable enzyme used in the extension phase of PCR for adding nucleotides.

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    Exponential Growth in PCR

    Each cycle of PCR doubles the amount of DNA, leading to billions of copies after multiple cycles.

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    Gel Electrophoresis

    Technique used to separate DNA fragments by size after PCR amplification.

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    DNA Profiling

    Analyzing variable DNA regions to create a unique genetic fingerprint for individuals.

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    Applications of PCR

    PCR can verify results, analyze genetic variations, and isolate DNA fragments for study.

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    Applications in Forensics

    Using DNA analysis from crime scenes for investigations, often via PCR and gel electrophoresis.

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    Overview of DNA Replication Enzymes

    Helicase unwinds DNA; DNA polymerase synthesizes new strands ensuring accurate base pairing.

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    Triplet Code

    A genetic code comprising three nucleotides that specifies an amino acid.

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    Degenerate Code

    Multiple codons can encode the same amino acid, providing genetic redundancy.

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    tRNA Function

    tRNA transports amino acids to the ribosome and helps form peptide bonds.

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    Ribosome Sites

    The ribosome has three sites: A site (aminoacyl), P site (peptidyl), E site (exit).

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    Peptide Bond Formation

    The chemical bond formed between amino acids during protein synthesis.

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    Universal DNA

    The genetic structure of DNA is consistent across all living organisms.

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    Conserved DNA

    DNA sequences that remain unchanged over generations due to essential functions.

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    Haemoglobin Role

    Hemoglobin is the protein in red blood cells that transports oxygen.

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    Transcription

    The synthesis of RNA using DNA as a template, occurring in the nucleus.

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    RNA polymerase

    An enzyme that synthesizes RNA by binding to DNA and unwinding it.

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    Coding strand

    The DNA strand that matches the RNA sequence except for T and U.

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    Transcriptomes

    The complete set of RNA transcripts produced by a cell.

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    Translation

    The synthesis of polypeptides from mRNA in the ribosomes.

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    Codons

    Sequences of three nucleotides on mRNA that specify amino acids.

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    Start codon

    The first codon in mRNA (AUG) that signals the beginning of translation.

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    Stop codon

    The codon that signals the end of translation.

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    Ribosomal complex

    The structure made of ribosomal subunits where translation occurs.

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    tRNA

    Transfer RNA that carries amino acids to the ribosome during translation.

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    Amino acids

    The building blocks of proteins, linked in specific sequences.

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    Nucleotide requirement

    To code for a polypeptide, 3 nucleotides are needed for each amino acid.

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    Gene expression regulation

    Not all genes are active at all times; some can be switched on or off.

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    DNA Identification

    Using DNA profiles to identify victims by matching samples.

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    Trace Evidence

    Small biological materials like hair amplified for identification.

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    Paternity Testing

    Comparing DNA from child, mother, and alleged father to establish relationships.

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    PCR Amplification

    Process that increases DNA quantity from small samples.

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    DNA Patterns

    Unique arrangements of DNA fragments visualized after separation.

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    DNA Ladder

    Reference mix of DNA fragments used for measuring sizes.

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    Electric Current in Gel

    Drives DNA migration during gel electrophoresis.

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    Restriction Enzymes

    Enzymes that cut DNA into smaller fragments.

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    Tandem Repeats

    Short sequences of DNA repeated in the genome, varying by individual.

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    Microsatellites

    Short tandem repeats of 1-6 base pairs in DNA.

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    Minisatellites

    Repeat sequences of 10-60 base pairs.

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    Macrosatellites

    Long tandem repeats of greater than 100 base pairs.

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    Visualization in Gel

    Using dyes to make DNA bands visible under UV light.

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    Size-Based Separation

    Smaller DNA fragments move faster in the gel than larger ones.

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    Study Notes

    DNA Replication Overview

    • DNA replication is crucial for cell division (growth and repair).
    • Ensures new cells inherit the correct genetic information for proper function.
    • Happens at a high speed (approximately one hour in human cells) while maintaining accuracy.
    • Allows for multicellular organism development from a single cell.
    • Essential for proper cell differentiation during repair.

    Semi-Conservative Replication

    • DNA replication is semi-conservative.
    • Each new DNA molecule has one original (parent) strand and one newly synthesized strand.
    • Parental strands act as templates.
    • Complementary base pairing (A-T, C-G) ensures accuracy.
    • Results in two identical DNA molecules.

    Helicase and DNA Polymerase in Replication

    • Helicase unwinds the DNA double helix by breaking hydrogen bonds between bases.
    • This action exposes the template strands for replication.
    • DNA Polymerase builds new strands using free nucleotides and follows the 5' to 3' direction, matching bases (A-T, C-G).
    • DNA polymerase has a proofreading mechanism to ensure accuracy.

    Polymerase Chain Reaction (PCR)

    • PCR amplifies specific DNA sequences.
    • Key Steps: Denaturation, Annealing, Extension.
    • Denaturation (98°C): Separates DNA strands using heat.
    • Annealing (60°C): Allows primers to bind to specific DNA targets.
    • Extension (72°C): Taq polymerase adds nucleotides to extend strands.
    • Each cycle doubles the DNA amount, resulting in exponential growth.
    • Essential for sufficient DNA in further analyses (e.g., gel electrophoresis).

    Gel Electrophoresis

    • Separates DNA fragments by size.
    • Smaller fragments migrate further through the gel than larger fragments.
    • DNA ladders (known fragment lengths) used to determine sample fragment sizes.
    • Crucial for visualizing DNA profiles and confirming PCR success.
    • Crucial in applications of DNA profiling.

    Tandem Repeats in DNA Profiling

    • Tandem repeats (short, repeated sequences) show variation between individuals.
    • Restriction enzymes cut DNA at specific locations creating fragments of different sizes.
    • PCR amplifies the fragments.
    • Separation occurs via Gel Electrophoresis.
    • Useful for forensic investigations and paternity testing.

    Transcription

    • Synthesizes RNA from DNA template.
    • Occurs in the nucleus.
    • Uses one DNA strand as a template.
    • RNA polymerase unwinds DNA and brings RNA nucleotides for complementary pairing.
    • Creates a new RNA strand complementary to the template.
    • Replicated parts of DNA can be copied many times until needed.

    Translation

    • Synthesis of polypeptides (proteins).
    • Uses mRNA as a guide.
    • Codons (3-nucleotide sequences) on mRNA specify amino acid order.
    • Ribosomes assemble polypeptides.
    • tRNAs deliver amino acids.
    • Start codon (AUG) begins translation and codes for methionine (Met).
    • Stop codons end translation.

    Degeneracy and Conserved DNA

    • Degeneracy: Multiple codons can code for the same amino acid.
    • Conserved DNA sequences remain unchanged over time due to their essential functions.

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    Description

    Explore the fundamental process of DNA replication, its significance in cell division, and how it maintains genetic accuracy. The semi-conservative nature of replication ensures that each new DNA molecule consists of one parent strand and one newly synthesized strand. Key enzymes like helicase and DNA polymerase play crucial roles in unwinding the DNA and synthesizing new strands.

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