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Questions and Answers
What is the primary purpose of DNA replication?
What is the primary purpose of DNA replication?
DNA replication is essential only during organism growth, not during tissue repair.
DNA replication is essential only during organism growth, not during tissue repair.
False (B)
What is meant by the term 'semi-conservative' in DNA replication?
What is meant by the term 'semi-conservative' in DNA replication?
It means each new DNA double helix contains one original strand and one newly synthesized strand.
During DNA replication, Adenine pairs with _____ and Cytosine pairs with _____.
During DNA replication, Adenine pairs with _____ and Cytosine pairs with _____.
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Match the following stages of life processes with their descriptions:
Match the following stages of life processes with their descriptions:
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How long does it typically take for DNA replication to occur in human cells?
How long does it typically take for DNA replication to occur in human cells?
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Fast DNA replication ensures trillions of identical cells can develop from one fertilized egg.
Fast DNA replication ensures trillions of identical cells can develop from one fertilized egg.
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What mnemonic can help remember the processes that depend on DNA replication?
What mnemonic can help remember the processes that depend on DNA replication?
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What term describes the phenomenon where multiple codons code for the same amino acid?
What term describes the phenomenon where multiple codons code for the same amino acid?
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The sequence of DNA varies significantly among all organisms.
The sequence of DNA varies significantly among all organisms.
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What is the function of haemoglobin in red blood cells?
What is the function of haemoglobin in red blood cells?
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The __________ is the site in the ribosome where tRNA first enters carrying its amino acid.
The __________ is the site in the ribosome where tRNA first enters carrying its amino acid.
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How many nucleotides are required to code for 240 amino acids?
How many nucleotides are required to code for 240 amino acids?
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Match the following ribosome sites with their functions:
Match the following ribosome sites with their functions:
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Conserved sequences are those that change rapidly across generations.
Conserved sequences are those that change rapidly across generations.
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Which proteins, besides haemoglobin, are not required in red blood cells?
Which proteins, besides haemoglobin, are not required in red blood cells?
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What is the primary purpose of gel electrophoresis in DNA profiling?
What is the primary purpose of gel electrophoresis in DNA profiling?
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Larger DNA fragments move farther in gel electrophoresis compared to smaller fragments.
Larger DNA fragments move farther in gel electrophoresis compared to smaller fragments.
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What method is used to amplify DNA from minute samples?
What method is used to amplify DNA from minute samples?
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The number of times a tandem repeat is repeated can vary widely between __________.
The number of times a tandem repeat is repeated can vary widely between __________.
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Match the following types of tandem repeats with their lengths:
Match the following types of tandem repeats with their lengths:
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What is the function of a DNA ladder in gel electrophoresis?
What is the function of a DNA ladder in gel electrophoresis?
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Restriction enzymes are used to amplify DNA fragments for analysis.
Restriction enzymes are used to amplify DNA fragments for analysis.
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In DNA profiling, what is compared to determine biological relationships?
In DNA profiling, what is compared to determine biological relationships?
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During __________, DNA moves toward the positive electrode due to its negative charge.
During __________, DNA moves toward the positive electrode due to its negative charge.
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Match the following terms with their descriptions:
Match the following terms with their descriptions:
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Why are tandem repeats useful in forensic investigations?
Why are tandem repeats useful in forensic investigations?
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Ethidium bromide is used to visualize DNA bands under infrared light.
Ethidium bromide is used to visualize DNA bands under infrared light.
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What role do restriction endonucleases play in DNA profiling?
What role do restriction endonucleases play in DNA profiling?
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The process of comparing the distance traveled by sample fragments to a DNA ladder is part of determining the __________ of DNA fragments.
The process of comparing the distance traveled by sample fragments to a DNA ladder is part of determining the __________ of DNA fragments.
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What is the main function of helicase in DNA replication?
What is the main function of helicase in DNA replication?
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DNA polymerase can initiate DNA synthesis without a primer.
DNA polymerase can initiate DNA synthesis without a primer.
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What base pairs with adenine (A) in DNA?
What base pairs with adenine (A) in DNA?
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The _____ enzyme synthesizes new DNA strands using the template.
The _____ enzyme synthesizes new DNA strands using the template.
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Match the following phases of PCR with their descriptions:
Match the following phases of PCR with their descriptions:
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Which phase of PCR is characterized by the temperature being raised to 98°C?
Which phase of PCR is characterized by the temperature being raised to 98°C?
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PCR can amplify DNA from minute samples to millions of copies.
PCR can amplify DNA from minute samples to millions of copies.
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What is the purpose of gel electrophoresis in PCR?
What is the purpose of gel electrophoresis in PCR?
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In eukaryotic DNA, the first step of replication involves the _____ of the double helix.
In eukaryotic DNA, the first step of replication involves the _____ of the double helix.
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Match the following enzymes with their roles:
Match the following enzymes with their roles:
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What does the acronym STR stand for in DNA profiling?
What does the acronym STR stand for in DNA profiling?
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In the PCR process, the extension phase uses Taq polymerase, which is heat-stable.
In the PCR process, the extension phase uses Taq polymerase, which is heat-stable.
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What is the significance of proof-reading in DNA polymerase?
What is the significance of proof-reading in DNA polymerase?
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Each cycle of PCR results in _____ the amount of DNA.
Each cycle of PCR results in _____ the amount of DNA.
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What role do primers play in PCR?
What role do primers play in PCR?
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What is the main function of RNA polymerase during transcription?
What is the main function of RNA polymerase during transcription?
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Transcription occurs in the cytoplasm in eukaryotic cells.
Transcription occurs in the cytoplasm in eukaryotic cells.
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What is the role of mRNA in protein synthesis?
What is the role of mRNA in protein synthesis?
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The start codon on mRNA is always _____ and it codes for _____ as the first amino acid.
The start codon on mRNA is always _____ and it codes for _____ as the first amino acid.
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Match the RNA structures to their functions:
Match the RNA structures to their functions:
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What distinguishes RNA from DNA?
What distinguishes RNA from DNA?
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Transcription can occur simultaneously on both strands of DNA.
Transcription can occur simultaneously on both strands of DNA.
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How many nucleotides are needed in the coding strand to produce a polypeptide of 240 amino acids?
How many nucleotides are needed in the coding strand to produce a polypeptide of 240 amino acids?
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The _____ gene may only be switched on during the summer.
The _____ gene may only be switched on during the summer.
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Match the following terms with their correct definitions:
Match the following terms with their correct definitions:
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During translation, which site on the ribosome is responsible for peptide bond formation?
During translation, which site on the ribosome is responsible for peptide bond formation?
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A single mRNA molecule can only be translated once.
A single mRNA molecule can only be translated once.
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What happens to the DNA after RNA polymerase leaves during transcription?
What happens to the DNA after RNA polymerase leaves during transcription?
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The _____ strand of DNA serves as the template during transcription.
The _____ strand of DNA serves as the template during transcription.
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What is the primary purpose of the ribosome in translation?
What is the primary purpose of the ribosome in translation?
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Flashcards
DNA replication
DNA replication
The process of copying DNA for cell division.
Cell division
Cell division
The process that results in two or more daughter cells from a parent cell.
Importance of accurate DNA copies
Importance of accurate DNA copies
Ensures new cells inherit correct genetic information.
Speed of DNA replication
Speed of DNA replication
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Semi-conservative replication
Semi-conservative replication
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Template strand
Template strand
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Complementary base pairing
Complementary base pairing
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Result of DNA replication
Result of DNA replication
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Role of Helicase
Role of Helicase
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Role of DNA Polymerase
Role of DNA Polymerase
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Base Pairing Rule
Base Pairing Rule
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PCR (Polymerase Chain Reaction)
PCR (Polymerase Chain Reaction)
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Denaturation Phase
Denaturation Phase
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Annealing Phase
Annealing Phase
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Extension Phase
Extension Phase
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Taq Polymerase
Taq Polymerase
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Exponential Growth in PCR
Exponential Growth in PCR
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Gel Electrophoresis
Gel Electrophoresis
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DNA Profiling
DNA Profiling
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Applications of PCR
Applications of PCR
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Applications in Forensics
Applications in Forensics
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Overview of DNA Replication Enzymes
Overview of DNA Replication Enzymes
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Triplet Code
Triplet Code
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Degenerate Code
Degenerate Code
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tRNA Function
tRNA Function
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Ribosome Sites
Ribosome Sites
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Peptide Bond Formation
Peptide Bond Formation
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Universal DNA
Universal DNA
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Conserved DNA
Conserved DNA
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Haemoglobin Role
Haemoglobin Role
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Transcription
Transcription
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RNA polymerase
RNA polymerase
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Coding strand
Coding strand
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Transcriptomes
Transcriptomes
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Translation
Translation
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Codons
Codons
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Start codon
Start codon
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Stop codon
Stop codon
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Ribosomal complex
Ribosomal complex
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tRNA
tRNA
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Amino acids
Amino acids
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Nucleotide requirement
Nucleotide requirement
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Gene expression regulation
Gene expression regulation
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DNA Identification
DNA Identification
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Trace Evidence
Trace Evidence
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Paternity Testing
Paternity Testing
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PCR Amplification
PCR Amplification
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DNA Patterns
DNA Patterns
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DNA Ladder
DNA Ladder
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Electric Current in Gel
Electric Current in Gel
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Restriction Enzymes
Restriction Enzymes
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Tandem Repeats
Tandem Repeats
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Microsatellites
Microsatellites
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Minisatellites
Minisatellites
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Macrosatellites
Macrosatellites
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Visualization in Gel
Visualization in Gel
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Size-Based Separation
Size-Based Separation
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Study Notes
DNA Replication Overview
- DNA replication is crucial for cell division (growth and repair).
- Ensures new cells inherit the correct genetic information for proper function.
- Happens at a high speed (approximately one hour in human cells) while maintaining accuracy.
- Allows for multicellular organism development from a single cell.
- Essential for proper cell differentiation during repair.
Semi-Conservative Replication
- DNA replication is semi-conservative.
- Each new DNA molecule has one original (parent) strand and one newly synthesized strand.
- Parental strands act as templates.
- Complementary base pairing (A-T, C-G) ensures accuracy.
- Results in two identical DNA molecules.
Helicase and DNA Polymerase in Replication
- Helicase unwinds the DNA double helix by breaking hydrogen bonds between bases.
- This action exposes the template strands for replication.
- DNA Polymerase builds new strands using free nucleotides and follows the 5' to 3' direction, matching bases (A-T, C-G).
- DNA polymerase has a proofreading mechanism to ensure accuracy.
Polymerase Chain Reaction (PCR)
- PCR amplifies specific DNA sequences.
- Key Steps: Denaturation, Annealing, Extension.
- Denaturation (98°C): Separates DNA strands using heat.
- Annealing (60°C): Allows primers to bind to specific DNA targets.
- Extension (72°C): Taq polymerase adds nucleotides to extend strands.
- Each cycle doubles the DNA amount, resulting in exponential growth.
- Essential for sufficient DNA in further analyses (e.g., gel electrophoresis).
Gel Electrophoresis
- Separates DNA fragments by size.
- Smaller fragments migrate further through the gel than larger fragments.
- DNA ladders (known fragment lengths) used to determine sample fragment sizes.
- Crucial for visualizing DNA profiles and confirming PCR success.
- Crucial in applications of DNA profiling.
Tandem Repeats in DNA Profiling
- Tandem repeats (short, repeated sequences) show variation between individuals.
- Restriction enzymes cut DNA at specific locations creating fragments of different sizes.
- PCR amplifies the fragments.
- Separation occurs via Gel Electrophoresis.
- Useful for forensic investigations and paternity testing.
Transcription
- Synthesizes RNA from DNA template.
- Occurs in the nucleus.
- Uses one DNA strand as a template.
- RNA polymerase unwinds DNA and brings RNA nucleotides for complementary pairing.
- Creates a new RNA strand complementary to the template.
- Replicated parts of DNA can be copied many times until needed.
Translation
- Synthesis of polypeptides (proteins).
- Uses mRNA as a guide.
- Codons (3-nucleotide sequences) on mRNA specify amino acid order.
- Ribosomes assemble polypeptides.
- tRNAs deliver amino acids.
- Start codon (AUG) begins translation and codes for methionine (Met).
- Stop codons end translation.
Degeneracy and Conserved DNA
- Degeneracy: Multiple codons can code for the same amino acid.
- Conserved DNA sequences remain unchanged over time due to their essential functions.
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Description
Explore the fundamental process of DNA replication, its significance in cell division, and how it maintains genetic accuracy. The semi-conservative nature of replication ensures that each new DNA molecule consists of one parent strand and one newly synthesized strand. Key enzymes like helicase and DNA polymerase play crucial roles in unwinding the DNA and synthesizing new strands.