DNA Replication Models Flashcards

In the dispersive model, DNA replication results in two DNA molecules that are mixtures, or hybrids, of parental and daughter DNA. Each strand is a patchwork of original and new DNA.

In the conservative model, one DNA molecule consists of both original strands and another molecule consists of two new strands that have the same sequences as the original.

In the Semi-Conservative model, the two strands of DNA unwind and each acts as a template for a new complementary strand, resulting in two DNA molecules, each containing one original strand and one new strand.

<p>E. coli bacteria.</p> Signup and view all the answers

<p>They grew E. coli in isotopes of Nitrogen, specifically 15N and 14N.</p> Signup and view all the answers

<p>An isotope is a version of an element that differs by the number of neutrons in its nucleus.</p> Signup and view all the answers

<ol> <li>Grow E. coli in medium containing 15N. 2. Allow bacterial cells to grow. 3. Measure DNA density after switching to 14N medium.</li> </ol> Signup and view all the answers

<p>15N is more dense than 14N.</p> Signup and view all the answers

<p>The bacteria synthesized new biological molecules, including DNA, using heavy 15N.</p> Signup and view all the answers

<p>It separates molecules into bands by spinning them in a density gradient formed by cesium chloride.</p> Signup and view all the answers

<p>A continuous density gradient is created.</p> Signup and view all the answers

<p>The molecules banded at equal densities, creating a 14N/15N duplex medium density.</p> Signup and view all the answers

<p>Nucleotide triphosphates.</p> Signup and view all the answers

<p>A highly reactive phosphate located on the 5' carbon.</p> Signup and view all the answers

<p>The oxygen on the 3' carbon.</p> Signup and view all the answers

<p>Any process where small molecules combine to create a large chainlike or network molecule, called a polymer.</p> Signup and view all the answers

<p>The polymerase enzyme.</p> Signup and view all the answers

<p>It creates the template that determines new strand order.</p> Signup and view all the answers

<p>It moves in a 5' to 3' direction.</p> Signup and view all the answers

<p>Incoming dNTPS.</p> Signup and view all the answers

<p>An RNA primer.</p> Signup and view all the answers

<p>5' to 3'.</p> Signup and view all the answers

<p>True</p> Signup and view all the answers

<p>DnaA, DnaB, and DnaC.</p> Signup and view all the answers

<p>They attach to a specific origin sequence and cause local denaturation.</p> Signup and view all the answers

<p>Helicase and primase.</p> Signup and view all the answers

<p>By DnaB and DnaC combining.</p> Signup and view all the answers

<p>It untwists the strands of DNA.</p> Signup and view all the answers

<p>The RNA primer that provides a 3' OH.</p> Signup and view all the answers

<p>Single strand binding proteins.</p> Signup and view all the answers

<p>They bind to exposed template strands and prevent degradation and renaturation.</p> Signup and view all the answers

<p>Reduces coiling ahead of the fork.</p> Signup and view all the answers