DNA Replication Mechanism Quiz
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Questions and Answers

Which enzyme replaces the RNA primer with a DNA sequence during DNA replication?

  • DNA helicase
  • DNA primase
  • DNA polymerase I (correct)
  • DNA ligase
  • What is the role of DNA ligase in DNA replication?

  • Cuts the sugar-phosphate backbone
  • Initiates DNA replication
  • Joins Okazaki fragments on the lagging strand (correct)
  • Separates DNA strands
  • Which enzyme relaxes supercoils by cutting the sugar-phosphate backbone during DNA replication?

  • DNA polymerase I
  • DNA helicase
  • DNA primase
  • DNA topoisomerase (correct)
  • What is the direction of DNA synthesis during replication?

    <p>5' to 3'</p> Signup and view all the answers

    What initiates the process of DNA replication?

    <p>DNA primase</p> Signup and view all the answers

    Which protein binds to the origin of replication during the initiation of DNA replication?

    <p>Initiator protein</p> Signup and view all the answers

    What is the function of Primase in DNA replication?

    <p>Synthesizes RNA primers</p> Signup and view all the answers

    Which enzyme catalyzes the formation of phosphodiester bonds between adjacent nucleotides in the new DNA strand?

    <p>DNA polymerase III</p> Signup and view all the answers

    In DNA replication, which strand has continuous synthesis in the direction of the replication fork?

    <p>Leading strand</p> Signup and view all the answers

    What are Okazaki fragments in the context of DNA replication?

    <p>Short DNA fragments on the lagging strand</p> Signup and view all the answers

    Which type of DNA synthesis direction is characteristic of the lagging strand during DNA replication?

    <p>3'-to-5'</p> Signup and view all the answers

    In the Meselson-Stahl experiment, how did they label the DNA in E. coli initially?

    <p>With 15N heavy isotope</p> Signup and view all the answers

    What forms a tight band at the position where its density equals the CsCl density in the Meselson-Stahl experiment?

    <p>DNA</p> Signup and view all the answers

    Where does DNA synthesis proceed from during replication?

    <p>5′ to 3′ direction</p> Signup and view all the answers

    What provides the energy for DNA synthesis during replication?

    <p>High-energy phosphate bonds associated with dNTPs</p> Signup and view all the answers

    Which enzyme catalyzes the formation of new phosphodiester bonds during DNA replication?

    <p>DNA polymerase</p> Signup and view all the answers

    Which stage of DNA replication involves opening up the double helix and preparing it for base pairing?

    <p>Initiation</p> Signup and view all the answers

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