DNA Replication Mechanism Quiz
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Questions and Answers

Which enzyme replaces the RNA primer with a DNA sequence during DNA replication?

  • DNA helicase
  • DNA primase
  • DNA polymerase I (correct)
  • DNA ligase

What is the role of DNA ligase in DNA replication?

  • Cuts the sugar-phosphate backbone
  • Initiates DNA replication
  • Joins Okazaki fragments on the lagging strand (correct)
  • Separates DNA strands

Which enzyme relaxes supercoils by cutting the sugar-phosphate backbone during DNA replication?

  • DNA polymerase I
  • DNA helicase
  • DNA primase
  • DNA topoisomerase (correct)

What is the direction of DNA synthesis during replication?

<p>5' to 3' (B)</p> Signup and view all the answers

What initiates the process of DNA replication?

<p>DNA primase (B)</p> Signup and view all the answers

Which protein binds to the origin of replication during the initiation of DNA replication?

<p>Initiator protein (C)</p> Signup and view all the answers

What is the function of Primase in DNA replication?

<p>Synthesizes RNA primers (C)</p> Signup and view all the answers

Which enzyme catalyzes the formation of phosphodiester bonds between adjacent nucleotides in the new DNA strand?

<p>DNA polymerase III (A)</p> Signup and view all the answers

In DNA replication, which strand has continuous synthesis in the direction of the replication fork?

<p>Leading strand (C)</p> Signup and view all the answers

What are Okazaki fragments in the context of DNA replication?

<p>Short DNA fragments on the lagging strand (B)</p> Signup and view all the answers

Which type of DNA synthesis direction is characteristic of the lagging strand during DNA replication?

<p>3'-to-5' (B)</p> Signup and view all the answers

In the Meselson-Stahl experiment, how did they label the DNA in E. coli initially?

<p>With 15N heavy isotope (C)</p> Signup and view all the answers

What forms a tight band at the position where its density equals the CsCl density in the Meselson-Stahl experiment?

<p>DNA (D)</p> Signup and view all the answers

Where does DNA synthesis proceed from during replication?

<p>5′ to 3′ direction (A)</p> Signup and view all the answers

What provides the energy for DNA synthesis during replication?

<p>High-energy phosphate bonds associated with dNTPs (D)</p> Signup and view all the answers

Which enzyme catalyzes the formation of new phosphodiester bonds during DNA replication?

<p>DNA polymerase (B)</p> Signup and view all the answers

Which stage of DNA replication involves opening up the double helix and preparing it for base pairing?

<p>Initiation (D)</p> Signup and view all the answers

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