17 Questions
Which enzyme replaces the RNA primer with a DNA sequence during DNA replication?
DNA polymerase I
What is the role of DNA ligase in DNA replication?
Joins Okazaki fragments on the lagging strand
Which enzyme relaxes supercoils by cutting the sugar-phosphate backbone during DNA replication?
DNA topoisomerase
What is the direction of DNA synthesis during replication?
5' to 3'
What initiates the process of DNA replication?
DNA primase
Which protein binds to the origin of replication during the initiation of DNA replication?
Initiator protein
What is the function of Primase in DNA replication?
Synthesizes RNA primers
Which enzyme catalyzes the formation of phosphodiester bonds between adjacent nucleotides in the new DNA strand?
DNA polymerase III
In DNA replication, which strand has continuous synthesis in the direction of the replication fork?
Leading strand
What are Okazaki fragments in the context of DNA replication?
Short DNA fragments on the lagging strand
Which type of DNA synthesis direction is characteristic of the lagging strand during DNA replication?
3'-to-5'
In the Meselson-Stahl experiment, how did they label the DNA in E. coli initially?
With 15N heavy isotope
What forms a tight band at the position where its density equals the CsCl density in the Meselson-Stahl experiment?
DNA
Where does DNA synthesis proceed from during replication?
5′ to 3′ direction
What provides the energy for DNA synthesis during replication?
High-energy phosphate bonds associated with dNTPs
Which enzyme catalyzes the formation of new phosphodiester bonds during DNA replication?
DNA polymerase
Which stage of DNA replication involves opening up the double helix and preparing it for base pairing?
Initiation
Test your knowledge about the mechanism of DNA replication, including the strict requirements for DNA polymerase action, the role of dNTPs, single-stranded template, and the initiation process involving initiator proteins and DNA helicase.
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