DNA Replication Mechanism Quiz

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Which enzyme replaces the RNA primer with a DNA sequence during DNA replication?

DNA helicase

DNA primase

DNA polymerase I (correct)

DNA ligase

What is the role of DNA ligase in DNA replication?

Cuts the sugar-phosphate backbone

Initiates DNA replication

Joins Okazaki fragments on the lagging strand (correct)

Separates DNA strands

Which enzyme relaxes supercoils by cutting the sugar-phosphate backbone during DNA replication?

DNA polymerase I

DNA helicase

DNA primase

DNA topoisomerase (correct)

What is the direction of DNA synthesis during replication?

5' to 3' Signup and view all the answers

What initiates the process of DNA replication?

DNA primase Signup and view all the answers

Which protein binds to the origin of replication during the initiation of DNA replication?

Initiator protein Signup and view all the answers

What is the function of Primase in DNA replication?

Synthesizes RNA primers Signup and view all the answers

Which enzyme catalyzes the formation of phosphodiester bonds between adjacent nucleotides in the new DNA strand?

DNA polymerase III Signup and view all the answers

In DNA replication, which strand has continuous synthesis in the direction of the replication fork?

Leading strand Signup and view all the answers

What are Okazaki fragments in the context of DNA replication?

Short DNA fragments on the lagging strand Signup and view all the answers

Which type of DNA synthesis direction is characteristic of the lagging strand during DNA replication?

3'-to-5' Signup and view all the answers

In the Meselson-Stahl experiment, how did they label the DNA in E. coli initially?

With 15N heavy isotope Signup and view all the answers

What forms a tight band at the position where its density equals the CsCl density in the Meselson-Stahl experiment?

DNA Signup and view all the answers

Where does DNA synthesis proceed from during replication?

5′ to 3′ direction Signup and view all the answers

What provides the energy for DNA synthesis during replication?

High-energy phosphate bonds associated with dNTPs Signup and view all the answers

Which enzyme catalyzes the formation of new phosphodiester bonds during DNA replication?

DNA polymerase Signup and view all the answers

Which stage of DNA replication involves opening up the double helix and preparing it for base pairing?

Initiation Signup and view all the answers

DNA Replication Mechanism Quiz

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