30 Questions
Where does DNA replication occur in prokaryotes and eukaryotes?
In the cytoplasm for prokaryotes and in the nucleus for eukaryotes
What is the main implication of the Watson and Crick DNA model for replication?
Make a complementary copy for each strand
Which model of DNA replication involves separating the two strands and making a complementary copy for each strand?
Semiconservative model
What is the result of DNA replication in terms of the old and new DNA strands?
Both strands of one copy would be entirely old DNA, while the other copy would have both strands of new DNA
What was the outcome of the Matthew Meselson & Frank Stahl experiment involving E. coli cultured in 15N and 14N mediums?
DNA replicated with a mixture of 15N and 14N isotopes
Which isotope of nitrogen is by far the most abundant?
14N
In which step of the Matthew Meselson & Frank Stahl experiment was E. coli cultured in a 15N medium?
Step 1
What does the term 'bidirectional' mean in the context of DNA replication?
'Bidirectional' means replication occurs in both directions along the DNA strand
What does the term 'conservative model' propose about DNA replication?
'Conservative model' proposes that one strand of the parent molecule is conserved in each new DNA molecule
What is the purpose of centrifuging the extracted DNA samples on a salt density gradient?
To separate DNA based on its density
In which medium did the DNA synthesized after 1 replication cycle have a higher density?
15N containing medium
After 2 replication cycles in a 14N medium, how many bands of DNA were observed?
2
Which model did the Meselson-Stahl experiment not fit, and why?
Conservative model, due to the presence of a single intermediate band after one generation
What did the dispersive model predict, and how did it compare to the observed results?
Predicted no change in DNA density over generations, but observed gradual decrease in density
Which type of DNA replication did the data from the Meselson-Stahl experiment support?
Semiconservative replication
What is the purpose of using CsCl salt solution in centrifugation?
To separate DNA based on its density
After 3 cycles in a 14N medium, how many bands of DNA were observed?
2
"Which type of replication did the dispersive model predict, and how did it compare to the observed results?"
"Predicted no change in DNA density over generations; observed gradual decrease in density"
Which of the following is NOT a proposed model for DNA replication?
Mosaic model
What is the main implication of the Watson and Crick DNA model for replication?
Make a complementary copy for each strand
Which step of the Matthew Meselson & Frank Stahl experiment involved culturing E. coli in a 15N medium?
Step 1
What is the result of DNA replication in terms of the old and new DNA strands?
DNA strands separate and a complementary strand is synthesized for each, so that sibling chromatids have one old and one new strand.
What is the main implication of the Meselson-Stahl experiment?
DNA replication is conservative
What does the dispersive model predict, and how did it compare to the observed results?
It predicted a single band of DNA of intermediate density in each generation, which was not observed
What was the outcome of the Matthew Meselson & Frank Stahl experiment involving E. coli cultured in 15N and 14N mediums?
After 2 replication cycles in a 14N medium, there were 2 bands, one of intermediate density and one of lighter density corresponding with 14N
What is the purpose of using CsCl salt solution in centrifugation?
To separate DNA based on its density
Which model did the Meselson-Stahl experiment not fit, and why?
The dispersive model because it predicted a single band of DNA of intermediate density, which was not observed
Which type of replication did the dispersive model predict, and how did it compare to the observed results?
The dispersive model predicted semi-conservative replication, but the observed results did not match this prediction
In which medium did the DNA synthesized after 1 replication cycle have a higher density?
15N medium
Which type of DNA replication did the data from the Meselson-Stahl experiment support?
Semiconservative replication
Test your knowledge of DNA replication in prokaryotes and eukaryotes with this quiz. Learn about the bidirectional nature of replication, Watson and Crick's model, and the three proposed models for DNA replication.
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