DNA Replication in Eukaryotes vs Prokaryotes Lecture
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Questions and Answers

What is the function of the origin recognition complex (ORC) in eukaryotic DNA replication?

  • It keeps the two DNA strands separated and protected from nucleases
  • It decreases the time needed for replication
  • It is responsible for unwinding and separating the two DNA strands
  • It achieves origin recognition (correct)

    • Which protein is responsible for unwinding and separating the two DNA strands during eukaryotic DNA replication?

  • SSB (single strand binding) proteins
  • RPA (replication protein A)
  • MCM (minichromosome maintenance complex) (correct)
  • ORC (origin recognition complex)

    • What is a characteristic feature of the origins of replication in eukaryotic DNA?

  • They are achieved by SSB proteins
  • They result in multiple replication forks or bubbles (correct)
  • They decrease the time needed for replication
  • They are rich in G-C base pairs

    • Which type of DNA polymerase is involved in eukaryotic DNA replication?

    <p>DNA polymerase α, δ, and ε</p> Signup and view all the answers

    What is the role of replication protein A (RPA) in eukaryotic DNA replication?

    <p>To keep the two DNA strands separated and protected from nucleases</p> Signup and view all the answers

    What is the molecular mechanism responsible for addressing the end replication problem in eukaryotic DNA?

    <p>Telomerase activity</p> Signup and view all the answers

    Which DNA polymerase is responsible for mitochondrial DNA synthesis in eukaryotes?

    <p>DNA polymerase γ</p> Signup and view all the answers

    What is the function of DNA polymerase δ in eukaryotes?

    <p>Synthesis of the lagging strand</p> Signup and view all the answers

    Which enzyme is responsible for the removal of RNA primers in eukaryotic DNA replication?

    <p>FEN1</p> Signup and view all the answers

    What is the function of telomerase in eukaryotic cells?

    <p>Elongation of telomeres</p> Signup and view all the answers

    What happens when telomeres are shortened beyond a critical length in eukaryotic cells?

    <p>The cell can no longer divide</p> Signup and view all the answers

    Which enzyme is responsible for joining ends of two segments of DNA by catalyzing the formation of phosphodiester bond?

    <p>DNA Ligase 1</p> Signup and view all the answers

    What is the function of PCNA (proliferating cell nuclear antigen) in eukaryotic DNA replication?

    <p>Acts as a sliding clamp</p> Signup and view all the answers

    What is the role of DNA topoisomerases in DNA replication?

    <p>Catalyzing removal of supercoils</p> Signup and view all the answers

    What is the function of DNA polymerase ε in eukaryotic cells?

    <p>Synthesis of the leading strand</p> Signup and view all the answers

    What happens when telomeres are shortened beyond a critical length in eukaryotic cells?

    <p>The cell can no longer divide</p> Signup and view all the answers

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