DNA Replication in Eukaryotes vs Prokaryotes Lecture

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What is the function of the origin recognition complex (ORC) in eukaryotic DNA replication?

It achieves origin recognition

Which protein is responsible for unwinding and separating the two DNA strands during eukaryotic DNA replication?

MCM (minichromosome maintenance complex)

What is a characteristic feature of the origins of replication in eukaryotic DNA?

They result in multiple replication forks or bubbles

Which type of DNA polymerase is involved in eukaryotic DNA replication?

DNA polymerase α, δ, and ε

What is the role of replication protein A (RPA) in eukaryotic DNA replication?

To keep the two DNA strands separated and protected from nucleases

What is the molecular mechanism responsible for addressing the end replication problem in eukaryotic DNA?

Telomerase activity

Which DNA polymerase is responsible for mitochondrial DNA synthesis in eukaryotes?

DNA polymerase γ

What is the function of DNA polymerase δ in eukaryotes?

Synthesis of the lagging strand

Which enzyme is responsible for the removal of RNA primers in eukaryotic DNA replication?

FEN1

What is the function of telomerase in eukaryotic cells?

Elongation of telomeres

What happens when telomeres are shortened beyond a critical length in eukaryotic cells?

The cell can no longer divide

Which enzyme is responsible for joining ends of two segments of DNA by catalyzing the formation of phosphodiester bond?

DNA Ligase 1

What is the function of PCNA (proliferating cell nuclear antigen) in eukaryotic DNA replication?

Acts as a sliding clamp

What is the role of DNA topoisomerases in DNA replication?

Catalyzing removal of supercoils

What is the function of DNA polymerase ε in eukaryotic cells?

Synthesis of the leading strand

What happens when telomeres are shortened beyond a critical length in eukaryotic cells?

The cell can no longer divide

Explore the differences in DNA replication mechanisms between eukaryotic and prokaryotic organisms in this informative lecture. Learn about the variations in DNA polymerases and other factors influencing the replication process.

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