DNA Replication Enzymes in Prokaryotes and Eukaryotes Part 1 Quiz
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Questions and Answers

What is the primary function of DNA polymerase I in E. coli?

  • To proofread and correct errors during DNA synthesis
  • To synthesize new DNA strands in the 3'->5' direction
  • To remove RNA primers during DNA replication (correct)
  • To repair damaged DNA

Which DNA polymerase in E. coli is responsible for the bulk of DNA replication?

  • DNA polymerase II
  • DNA polymerase III (correct)
  • DNA polymerase I
  • DNA polymerase IV

What is the primary function of DNA polymerase IV in E. coli?

  • To remove RNA primers during DNA replication
  • To participate in DNA repair processes (correct)
  • To replicate DNA during normal cell division
  • To proofread and correct errors during DNA synthesis

Which DNA polymerase in E. coli has 3'->5' exonuclease (proofreading) activity?

<p>Both DNA polymerase I and DNA polymerase III (C)</p> Signup and view all the answers

What is the role of the $\epsilon$ (dnaQ) subunit in the E. coli DNA polymerase III holoenzyme?

<p>It provides the 3'-&gt;5' exonuclease (proofreading) activity (C)</p> Signup and view all the answers

Which DNA polymerase in E. coli is primarily responsible for DNA repair processes?

<p>DNA polymerase IV and DNA polymerase V (A)</p> Signup and view all the answers

Which pathway enables most organisms to synthesize nucleotides from nucleosides or bases available through diet or degradation of nucleic acids?

<p>Salvage pathway (B)</p> Signup and view all the answers

In animals, which route provides the major source of bases for nucleotide synthesis?

<p>Extracellular hydrolysis of ingested nucleic acids (A)</p> Signup and view all the answers

Which enzyme is involved in the de novo pathway of Pyrimidine Biosynthesis?

<p>Orotate phosphoriboxyltransferase (B)</p> Signup and view all the answers

What is the key function of DHO dehydrogenase in nucleotide biosynthesis?

<p>Oxidation of DHO to form orotate (C)</p> Signup and view all the answers

Which enzyme catalyzes the formation of Carbamoyl phosphate in the de novo pathway of Pyrimidine Biosynthesis?

<p>Carbamoyl phosphate synthase II (C)</p> Signup and view all the answers

What is the primary difference between de novo and salvage pathways in nucleotide biosynthesis?

<p>De novo pathways use precursors directly, while salvage pathways recycle nucleotide breakdown products. (B)</p> Signup and view all the answers

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