DNA Recombination Process Quiz
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Questions and Answers

Who invented the microscope and described the first microbes?

  • Lazzaro Spallanzani
  • Francesco Redi
  • Robert Hooke (correct)
  • Antonie van Leeuwenhoek

Which scientist observed the first single-celled organisms, such as bacteria?

  • Francesco Redi
  • Antonie van Leeuwenhoek (correct)
  • Lazzaro Spallanzani
  • Robert Hooke

Who conducted the 'meat/flies experiment' that disproved the idea of spontaneous generation for large organisms?

  • Robert Hooke
  • Francesco Redi (correct)
  • Lazzaro Spallanzani
  • Antonie van Leeuwenhoek

Which scientist sterilized meat broth by boiling, demonstrating that it failed to grow microbes?

<p>Lazzaro Spallanzani (C)</p> Signup and view all the answers

What theory proposed that living creatures could arise without parents?

<p>Spontaneous Generation (D)</p> Signup and view all the answers

'Spontaneous Generations' was disproved by which scientist's experiment involving meat and flies?

<p>Francesco Redi (D)</p> Signup and view all the answers

Who disproved the theory of spontaneous generation through the Swan-necked flask experiment?

<p>Louis Pasteur (D)</p> Signup and view all the answers

What does each colony/spot of bacterial growth represent?

<p>A clonal population replicating from one original cell (A)</p> Signup and view all the answers

Who discovered the solid medium using agar for bacterial culture to grow?

<p>Angelina &amp; Walther Hesse (A)</p> Signup and view all the answers

Which postulate of Koch's postulates states that the suspected organism should be present in ALL cases of the disease & absent from healthy individuals?

<p>Postulate #1 (A)</p> Signup and view all the answers

What is the main limitation of Koch's postulates in identifying pathogens and diseases?

<p>Pathogens may not be culturable with known techniques (A)</p> Signup and view all the answers

What is stromatolite clear fossilized evidence of?

<p>Bacterial communities (A)</p> Signup and view all the answers

What is the prebiotic soup a hypothetical set of conditions for?

<p>Formation of small organic molecules on early Earth (D)</p> Signup and view all the answers

What was produced by the Miller-Urey experiment under simulated prehistoric Earth conditions?

<p>&quot;Molecules of life&quot; like amino acids and their derivatives (B)</p> Signup and view all the answers

What model suggests self-sufficient abiotic chemical reactions formed cellular metabolism?

<p>Metabolist Model (B)</p> Signup and view all the answers

Which molecule is suggested to be a precursor to DNA and has catalytic activity?

<p>RNA (C)</p> Signup and view all the answers

What does the molecular clock measure?

<p>Time information contained in macromolecular sequence (A)</p> Signup and view all the answers

What does the Shine-Dalgarno sequence allow on mRNA?

<p>Binding to ribosomes in prokaryotes (B)</p> Signup and view all the answers

What is the main point of the endosymbiont theory?

<p>Eukaryotic cells originated from prokaryotic organisms (B)</p> Signup and view all the answers

What is a characteristic of bacterial genomes?

<p>Contain a significant amount of non-coding DNA (D)</p> Signup and view all the answers

Which process involves the transfer of DNA from the environment into a bacterial cell?

<p>Transformation (C)</p> Signup and view all the answers

What is the main function of bacteriophages?

<p>Inject viral DNA into bacterial cells (B)</p> Signup and view all the answers

Which statement best describes horizontal gene transfer in bacteria?

<p>Genes are acquired from the environment by the bacteria themselves (B)</p> Signup and view all the answers

What is a characteristic of gram-negative bacteria?

<p>Enhanced competence due to low temperature (B)</p> Signup and view all the answers

What is the fate of most foreign DNA entering a bacterial cell?

<p>Degradation by restriction endonucleases (B)</p> Signup and view all the answers

What is the role of Recombination proteins (Rec) in bacteria?

<p>Mediate the combination of two DNA molecules with specific homologous sequences (B)</p> Signup and view all the answers

What is the primary function of operons in bacteria?

<p>Regulate the expression of structural genes through DNA control sequences (B)</p> Signup and view all the answers

What is an example of genomic island regions in bacterial genomes?

<p>Regions with signs of horizontal gene transfer (B)</p> Signup and view all the answers

What is a characteristic feature of the nucleoid in bacterial cells?

<p>Forms loops of chromosomes called domains (A)</p> Signup and view all the answers

How do mitochondria behave like endosymbiotic organisms?

<p>Live inside another organism and reproduce independently (B)</p> Signup and view all the answers

During DNA replication, which enzyme is responsible for synthesizing new DNA strands?

<p>DNA polymerase III (D)</p> Signup and view all the answers

What is the primary function of RecA during DNA recombination?

<p>Invading recipient &amp; donor to homologous stretch of recipient (D)</p> Signup and view all the answers

Which enzyme catalyzes the breaking and rejoining event that passes the chromosomes at the termination of DNA replication?

<p>Topoisomerase IV (A)</p> Signup and view all the answers

What is the function of RNase H during the completion of DNA replication?

<p>Removes the RNA primers (D)</p> Signup and view all the answers

What is the primary role of DNA primase in DNA replication?

<p>Producing RNA primers (C)</p> Signup and view all the answers

Which type of mutation involves a change in a single base?

<p>Transition (D)</p> Signup and view all the answers

What is the function of photolyase enzyme in DNA repair?

<p>Binding to pyrimidine dimer and cleaving the cyclobutane ring under UV radiation (B)</p> Signup and view all the answers

What is the primary function of RecBCD protein machine in DNA recombination?

<p>Unwinding donor DNA (A)</p> Signup and view all the answers

What type of replication occurs for cellular DNA in most cases?

<p>Semiconservative (D)</p> Signup and view all the answers

Which enzyme is responsible for keeping the single DNA strands apart during DNA synthesis?

<p>SSBs (single-stranded DNA binding proteins) (A)</p> Signup and view all the answers

What do base analogs do during DNA replication?

<p>Introduce mutations due to incorrect pairing (C)</p> Signup and view all the answers

What type of mutation changes the amino acid sequence to a stop codon?

<p>Nonsense mutation (D)</p> Signup and view all the answers

What is the function of the O-specific polysaccharide in Lipopolysaccharide (LPS)?

<p>It forms a protective and stabilizing layer (C)</p> Signup and view all the answers

Which type of transport involves the movement of molecules against the gradient and requires energy?

<p>Active transport (C)</p> Signup and view all the answers

What is the main function of ABC Transporters?

<p>Provide energy for primary active transport (D)</p> Signup and view all the answers

What is the role of the phosphotransferase system (PTS) in bacterial cells?

<p>Drive entry of glucose by modifying it to G6P (C)</p> Signup and view all the answers

Which system is responsible for the transport of unfolded proteins requiring ATP hydrolysis to move out of the cell?

<p>Sec System (D)</p> Signup and view all the answers

What is the main function of the Sec-dependent secretion proteins?

<p>Move signal-peptide containing proteins into periplasm (B)</p> Signup and view all the answers

Which bacterial secretion system is used to inject toxins/harmful molecules into other cells?

<p>Type VI (C)</p> Signup and view all the answers

What is the main function of Glycocalyx?

<ul> <li>It serves as a network of polysaccharides from cell surface (B)</li> </ul> Signup and view all the answers

What does the Proton-Motive Force promote?

<ul> <li>Movement of protons across membranes downhill the electrochemical potential (B)</li> </ul> Signup and view all the answers

What is the function of Capsule in bacterial cells?

<ul> <li>It forms an organized glycocalyx that is difficult to remove (A)</li> </ul> Signup and view all the answers

What is the purpose of Type III bacterial secretion system?

<ul> <li>Inject toxins/harmful molecules into other cells (A)</li> </ul> Signup and view all the answers

Which repair mechanism recognizes and repairs bases that (mostly) do not distort DNA structure?

<p>Base-excision repair (A)</p> Signup and view all the answers

What is the function of DNA ligase in the excision repair process?

<p>Seals the broken ends of DNA back together (B)</p> Signup and view all the answers

In the SOS response, what is the role of RecA?

<p>Inactivates LexA when DNA is damaged (B)</p> Signup and view all the answers

Which process requires homology and is RecA mediated?

<p>Double-strand break repair (A)</p> Signup and view all the answers

What is the function of Rho in Rho-dependent termination?

<p>Binds mRNA &amp; moves along (C)</p> Signup and view all the answers

What is the role of Sigma70 factor in transcription?

<p>Needed for gene homeostasis (C)</p> Signup and view all the answers

Which structure serves as a permeability barrier in bacterial cells?

<p>Cell membrane (D)</p> Signup and view all the answers

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