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DNA Fingerprinting and Forensic Analysis

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DNA Fingerprinting and Forensic Analysis

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Questions and Answers

What is the year in which DNA Fingerprinting was introduced?

1990

1985 (correct)

1995

1980

What is the process by which DNA fragments are separated based on their size?

DNA transfer

DNA cutting

Gel electrophoresis (correct)

Electrophoresis

What is the term for the variations in DNA nucleotide sequences that generate fragment length differences?

Restriction Fragment Length Variation

Restriction Fragment Length Polymorphism (correct)

DNA Fragment Length Polymorphism

DNA Fragment Length Variation

What is the purpose of using restriction enzymes in DNA profiling?

To cut DNA into fragments Signup and view all the answers

What is the first step involved in DNA profiling?

DNA extraction Signup and view all the answers

What is the term that defines the intersection of law and science?

Forensic science Signup and view all the answers

What is the purpose of heating the sample to 94°C in a PCR reaction?

To denature the double-stranded template DNA Signup and view all the answers

What is the role of Taq DNA polymerase in PCR?

To add nucleotides to the growing strand of DNA Signup and view all the answers

What is the purpose of the thermal cycler in PCR?

To repeat the denaturing, annealing, and elongating temperatures Signup and view all the answers

What is the result of PCR amplification?

The number of copies of the target is doubled every cycle Signup and view all the answers

What is the purpose of DNA primers in PCR?

To flank the target region to be amplified Signup and view all the answers

What is one of the applications of PCR in forensic science?

Victim identification at a crime scene Signup and view all the answers