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DNA Extraction & PCR Identification of E. coli in Diarrheagenic Stool

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DNA Extraction & PCR Identification of E. coli in Diarrheagenic Stool

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Questions and Answers

What is the primary advantage of using specific primers in PCR?

To increase the length of the DNA strands

To reduce the stability of the primers

To decrease the number of copies of the target region

To selectively amplify particular DNA fragments (correct)

What is the effect of the primer's short length on its binding?

It makes the primer more prone to interactions with other sequences

It has no effect on the primer's binding

It makes the primer less prone to nonspecific binding (correct)

It increases the likelihood of nonspecific binding

During which step of the PCR program do the primers bind to their complementary sequences on the DNA template?

Denaturation

Amplification

Annealing (correct)

Extension

What is the purpose of the PCR program in this experiment?

To identify the presence of virulent genes in E. coli Signup and view all the answers

What is the length of the primers typically used in PCR?

18-25 nucleotides Signup and view all the answers

What is the concentration of the primers used in this experiment?

10 pmol Signup and view all the answers

What is the purpose of the housekeeping gene uidA in this experiment?

To act as a control gene in all E. coli isolates Signup and view all the answers

What is the result of the amplification step in PCR?

Millions of copies of the target region are produced Signup and view all the answers

Why is the temperature lowered during the annealing step?

To allow the primers to anneal to their complementary sequences Signup and view all the answers

What is the purpose of dividing the students into four groups?

To perform a PCR for each pair of primers Signup and view all the answers