DNA and Proteins: Structure and Function

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Questions and Answers

Which statement accurately describes the diagram, representing a portion of a nucleic acid?

  • It is an RNA molecule, as RNA exclusively contains adenine.
  • It is a DNA molecule, because DNA exclusively contains thymine.
  • It is a DNA molecule, as DNA exclusively contains adenine.
  • It is an RNA molecule, because only RNA contains thymine.

In a DNA molecule, which diagram correctly shows the arrangement of components in a nucleotide pairing with a guanine-containing nucleotide?

  • Sugar - Phosphate - Cytosine
  • Phosphate - Sugar - Cytosine
  • Phosphate - Sugar - Adenine
  • Sugar - Phosphate - Adenine

Which option correctly identifies the sugar type, the structure, and the nitrogenous bases present in a DNA molecule?

  • Deoxyribose, single-stranded, A, U, C, G
  • Ribose, double-stranded, A, T, C, G
  • Ribose, single-stranded, A, U, C, G
  • Deoxyribose, double-stranded, A, T, C, G

In the segment of DNA depicted, which combination correctly identifies the labeled components?

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Which cellular process involves the pairing of cytosine, thymine, and adenine with guanine, adenine, and thymine, respectively?

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Which option correctly identifies the packaging, shape, and location of a chromosome in a prokaryotic cell?

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A cell with non-radioactive DNA replicates in a medium with radioactive nucleotides. Which diagram represents DNA composition in the resulting daughter cells?

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Which combination correctly identifies the cell type, DNA structure, and DNA location in a cell?

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Which statement accurately describes DNA in cells?

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A strand of DNA has the base sequence ATGTCCG. What base sequence would result after semi-conservative replication?

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Which row identifies the result and the name of the essential process involved in the synthesis of new cells in an organism?

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What is the role of restriction enzymes and probes in genetic engineering?

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Erythromycin interferes with protein synthesis in bacterial cells. What process is likely affected by erythromycin?

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In an mRNA strand, 30% of bases are uracil, 10% are cytosine, and 20% are adenine. What percentage of bases on the correlating DNA strand would be cytosine?

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Given the mRNA sequence UAC CCG AAU UAG, what DNA sequence would be transcribed to produce this mRNA?

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A length of mRNA attached to a ribosome codes for a polypeptide that is 120 amino acids long. How many mRNA nucleotides code for the amino acids in this polypeptide?

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Small RNA pieces, called RNA interference (RNAi), prevent specific gene expression. What modification enable RNAi to enter cells for this?

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The sequence AUAGCCCUAGGC was transcribed from a nucleic acid. Which sequence is the template for this transcription?

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A length of DNA codes for part of a polypeptide and contains 633 base pairs. What is the most likely base pair count in associated codons and amino acids?

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The table shows combinations of a gene section and how it is processed. Which combination is correct?

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Which statement about ribosomal RNA (rRNA) is not correct?

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The diagram shows process A, compound B, and structures 1 and 2 in a cell. Identify process A, compound B, and structures 1 and 2.

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The diagram shows protein manufacture in a eukaryotic cell. Which combination correctly matches name of chemical structure A, process 1 location, and macromolecule D?

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A small protein section has the amino acid sequence glutamine-lysine-proline-valine-threonine. Which DNA base codes to produce this section of protein?

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A scientist identified a sequence of bases on a fragment: AGA UGU GCC GAA, coding for arginine, cysteine, alanine, and glutamic acid. What alternatives identify the nucleic acid type, its complementary DNA base sequence, and tRNA anticodon?

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Which statements correctly identifies presence or absence of introns and exons in a number, and the function of a gene?

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The coding strand of a gene is 15% adenine and 35% cytosine. What best describes composition of the transcribed mRNA strand?

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Refer to the following diagram that shows the enzyme aminoacyl-tRNA synthetase binding to a tRNA molecule and an amino acid. What process is shown?

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Scientists needed to identify genes w/ different DNA methylation patterns in dairy cows to test milk production. What cell location has the first step in gene product synthesis?

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A genetic code, based on reading the mRNA sequence four bases at a time, was recently developed. How many different codons are possible in the quadruplet codon system?

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Flashcards

What is DNA?

A molecule that carries genetic information in all living organisms.

What are DNMTs?

The enzyme that adds methyl groups to DNA, affecting gene expression.

What is DNA replication?

A process where one strand acts as a template, resulting in two identical DNA molecules.

What is mRNA?

A single-stranded nucleic acid that carries genetic information from DNA to ribosomes for protein synthesis.

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What is Transcription?

A process where genetic information is copied from DNA to mRNA.

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What is a ribosome?

A cellular structure where proteins are synthesized.

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What is Translation?

A process in which ribosomes use mRNA to assemble amino acids into a polypeptide chain.

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What are restriction enzymes?

Enzymes that cut DNA at specific sequences, used in genetic engineering.

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What are heat-resistant enzymes?

Enzymes able to withstand high temperatures and used in PCR.

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What is Polymerase Chain Reaction (PCR)?

A laboratory technique used to amplify specific DNA regions.

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What is Gel electrophoresis?

Technique that separates DNA fragments based on size.

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What is a probe?

A molecule that binds to a specific DNA sequence to locate a gene.

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What is a mutation?

Alteration in the nucleotide sequence of DNA.

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What is Genetic Engineering?

A technique used to modify genes in living organisms.

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What is a vector?

A plasmid, used to insert foreign DNA into host cells.

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What is Transgenic?

An organism whose genetic material has been altered using genetic engineering techniques.

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What are Caspases?

Enzymes that bind to DNA and assist with controlled cell self-destruction

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What is an active site?

The region on an enzyme that binds to the substrate.

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What is CRISPR cas9?

A method of in-activating selected gene.

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What is Enzyme catalysis?

The force by an enzyme to lower activation energy to improve reactions.

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Study Notes

  • The following outlines DNA and proteins.

DNA Structure, Function, and Replication

  • Diagrams can represent parts of nucleic acids showing components like cytosine, sugar, adenine, and thymine.
  • The diagram represents part of a DNA molecule because DNA contains thymine. Diagrams showing components of a nucleotide feature a sugar, phosphate and a nitrogenous base.
  • The arrangement shows how components pair in a DNA molecule with a nucleotide containing guanine
  • Key components: deoxyribose sugar, double stranded structure, and the bases adenine, thymine, cytosine, and guanine define DNA.
  • Symbols in diagrams denote sugar, phosphate, thymine (T), adenine (A), cytosine (C), and Guanine (G).
  • Replication of DNA involves pairing bases such as cytosine, thymine, and adenine with guanine, adenine, and thymine.

Packaging, Shape, and Location of Genetic Material

  • A chromosome in a prokaryotic cell in unbound, circular and it's located in the cytosol.

Conservative Replication

  • DNA information passes between generations through semi-conservative replication.
  • Given a DNA sequence ATGTCCG, a final strand features the sequence TACAGGC.

Replication Processes

  • The process is semi-conservative replication and it results in identical sister chromatids.

DNA Methylation

  • Semi-conservative replication, absent DNMTs, fails to maintain cell DNA methylation patterns.

Directionality of DNA in Transcription

  • The arrangement of the molecule and transcription relies on understanding directionality.

Genes and Protein Synthesis

  • Erythromycin interferes with protein bacterial cell synthesis by affecting the movement of ribosomes along mRNA.
  • Given an mRNA strand with 30% uracil (U), 10% cytosine (C), and 20% adenine (A), the DNA transcribed features 20% cytosine.

mRNA Molecule Sequencing

  • mRNA sequence: UAC CCG AAU UAG.
  • DNA sequence: ATG GGC TTA ATC.

Polypeptide Coding

  • A 120 amino acid polypeptide is coded by 360 mRNA nucleotides that are attached to a ribosome .

Expressing Specific Genes

  • Expressing specific genes involves the use of small pieces of the RNA called RNA interference (RNAi),.
  • Modified RNAi, as opposed to other forms of it, could enter a cell through the membrane.

Template-Based Transcription

  • Template = where sequence of bases transcribes from a nucleic acid.
  • Sequence: AUAGCCCUAGGC.
  • Sequence: TATCGGGATCCG.

DNA Length, Codons, and Amino Acids

  • For a DNA length with 633 base pairs codes for a polypeptide, 211 codons and 211 amino acids are likely involved.
  • An exon features transcription and translation.

Ribosomal RNA (rRNA)

  • Ribosomal RNA, or rRNA, possesses these characteristics:
  • Is found in both prokaryotic and eukaryotic cells
  • Help binds to the formation of poly peptides
  • mRNA (messenger RNA) is produced during transcription, then it binds to the bases.

diagrammatic Representation Cellular activity

  • diagrams show representation cellulare activity and chemical composition activity
  • Process A Transcription and Compound BmRNA both Ribosomes is the result/structure2, DNA the structure1.

Mammalian Protein Manufacture

  • The base sequence U A U will have results to a tRNA code of tyrosine by B15.

Gene Expression Mutations

  • Mutations prevented by gene one would then mostly affect or prevent this action from happening.

Coding Strand Anticipation and corresponding action

  • The anticodon then can give of in from this strand with base a sequence AGC is UGC.

Protein Coding

  • Genes = codes for a particular RNA

Coding strand base composition

  • Coding with an adeniase base of with 15%, cytosine composes about 35%.

Transferring Protein enzyme binding

  • Transferring an enzyme from the amino acide with an acid bind synthetase and transfer a molecule into it
  • As tRNA combines with molecule acids with amino then synthetrase is matched is its acids.

Genetics in connection to the enzyme and molecules

  • The acid chain at the start is then what the acid or synthetase and the atp will connect with before an amino

Genetically testing

  • Identify where these cells have been change over the gene production and also check if there are cells from the cells or any particular test
  • Cell division or change needs to occuor in the correct order

Genetic codes and test

  • Scientists codes can base of there mRNA to test and code for the quadruped

Tithin for cell composition

  • titithin is the largest function in of moleclecuar the humna or other animal like the rat, which then can can result to mutation

The cell cycle

  • The cell cycle needs the production of energy the cells needs the the molecelaur enegy needs also

Protein Structure and Function

  • Biochemical is a pathway in some human and then the process may results
  • Reaction catalyzed enzymes like result products

Protein and Enzyme Reaction

  • Drug used to describe a blood the protein

Altering Shapes

  • Altering to moleculaur for the the shape change

Temperatures enzyme

  • Denaturs or can also not form the the structure needed for other structures

MethylAmine Enzymes

  • The cell can react with molecule or may create some

Compund blocking enzmes

  • Blocking moleculaur enzyme is needed for the growth rate may act a reaction

Following graph which shows temp effects to an Enzy reaction

  • Rate reaction for a reaction by enzyme

Aminoacid structure

  • 4 aminobenzioc are with molecules needed or a part of with

Specificity

  • Specificity substrate molecule is a determined one

Enzymic reaction

  • Activatin energy and can come from heat or radiation

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