DNA and Gene Expression Quiz

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Questions and Answers

What role do transcription factors play in the initiation of transcription?

  • They elongate the RNA strand during transcription.
  • They assist RNA polymerase in recognizing and binding to the promoter. (correct)
  • They directly synthesize mRNA from the coding strand.
  • They help to process the pre-mRNA into mature mRNA.

What role does RNA play in the process of gene expression?

  • RNA acts as an intermediary between genes and proteins. (correct)
  • RNA is a direct template for amino acid synthesis.
  • RNA serves solely as a structural component of ribosomes.
  • RNA carries genetic information from proteins to DNA.

During RNA elongation, which strand is used as the template for mRNA synthesis?

  • The non-template strand.
  • The coding strand.
  • The template strand. (correct)
  • Both strands are used.

Which of the following characteristics of the genetic code indicates that it contains repeated sequences for coding the same amino acid?

<p>Redundancy (C)</p> Signup and view all the answers

What is the primary function of the spliceosome in eukaryotic cells?

<p>To remove introns from pre-mRNA. (D)</p> Signup and view all the answers

Which sequence correctly reflects the central dogma of molecular biology?

<p>DNA is transcribed into RNA, which is then translated into proteins. (C)</p> Signup and view all the answers

Which modification is crucial for mRNA to exit the nucleus and bind to ribosomes?

<p>Addition of a modified guanosine cap to the 5’ end. (D)</p> Signup and view all the answers

What is the primary function of the start codon during protein synthesis?

<p>To initiate the binding of tRNA to mRNA. (D)</p> Signup and view all the answers

Which of the following statements about alternative splicing is true?

<p>It allows for the formation of multiple proteins from a single gene by including various combinations of exons. (A)</p> Signup and view all the answers

Which feature of mRNA allows it to bind specifically to the tRNA anticodon?

<p>The complementary base pairing of codons and anticodons. (A)</p> Signup and view all the answers

Which amino acid corresponds to the anticodon U G?

<p>Trytophan (A)</p> Signup and view all the answers

What type of bond is represented between the amino acids in the diagram?

<p>Peptide bond (A)</p> Signup and view all the answers

In the sequence 5' C A U C U G 3', which nucleotide appears first?

<p>Cytosine (A)</p> Signup and view all the answers

Which amino acid is incorrectly matched with its codon based on the provided sequence?

<p>Alanine with G U A (B)</p> Signup and view all the answers

What is the direction of transcription as indicated by the notation in the sequence?

<p>From 5' to 3' (B)</p> Signup and view all the answers

Which of the following pairs of amino acids are represented in the provided diagram?

<p>Ser and Ala (B)</p> Signup and view all the answers

In the context of tRNA, what does the 'G' nucleotide represent in the anticodon G U A?

<p>Valine (B)</p> Signup and view all the answers

Which sequence represents an incorrect 5' to 3' layout based on common base pairing rules?

<p>5' A U G C 3' (A)</p> Signup and view all the answers

Which region of tRNA is specifically responsible for binding to the amino acid?

<p>The 3′ single-stranded region (A)</p> Signup and view all the answers

What is the role of the release factor during translation?

<p>It triggers termination at the stop codon. (B)</p> Signup and view all the answers

What role does the anticodon of tRNA play during protein synthesis?

<p>Binds to the corresponding codon in mRNA (B)</p> Signup and view all the answers

Which statement accurately describes a common feature of all tRNAs?

<p>They all contain a stem-loop structure (B)</p> Signup and view all the answers

At which site does the tRNA–amino acid initially bind during elongation?

<p>A site (D)</p> Signup and view all the answers

How many tRNAs can be accommodated at the ribosome during the elongation process?

<p>Two tRNAs simultaneously. (C)</p> Signup and view all the answers

In the context of tRNA, what does the term 'codon' refer to?

<p>A sequence of three nucleotides on mRNA (D)</p> Signup and view all the answers

Which component is NOT part of the tRNA structure?

<p>Ribosome binding site (C)</p> Signup and view all the answers

What happens to the tRNA that is considered 'empty' during the elongation phase?

<p>It exits from the E site of the ribosome. (A)</p> Signup and view all the answers

What is the function of the anticodons in tRNA?

<p>To pair with codons on the mRNA. (A)</p> Signup and view all the answers

What determines the specific amino acid that tRNA carries?

<p>The sequence of the anticodon (D)</p> Signup and view all the answers

Which step follows the formation of a peptide bond between an amino acid and the growing polypeptide chain?

<p>The ribosome moves forward along the mRNA. (A)</p> Signup and view all the answers

What is the primary function of the hydrogen bonds in the tRNA structure?

<p>To form the 3D shape required for function (C)</p> Signup and view all the answers

Which of the following correctly characterizes the relationship between tRNA and protein synthesis?

<p>tRNA brings amino acids to the ribosome for protein assembly (C)</p> Signup and view all the answers

What is indicated by the presence of a stop codon on the mRNA during translation?

<p>Termination of protein synthesis. (A)</p> Signup and view all the answers

Which part of the ribosome accommodates the growing peptide chain?

<p>P site (D)</p> Signup and view all the answers

What role does the P site play in a ribosome during translation?

<p>It holds the tRNA that carries the growing polypeptide chain. (A)</p> Signup and view all the answers

During which stage of translation does the ribosome disassemble and release the completed polypeptide?

<p>Termination (D)</p> Signup and view all the answers

Which of the following statements is correct about the initiation stage of translation?

<p>The first tRNA carries the amino acid methionine. (D)</p> Signup and view all the answers

What describes the function of the A site in the ribosome?

<p>It is the site for the next tRNA to bind. (D)</p> Signup and view all the answers

Which of the following accurately describes the components of ribosomes?

<p>They contain large and small subunits composed of rRNA and proteins. (D)</p> Signup and view all the answers

What occurs during the elongation stage of translation?

<p>Synthesis of the polypeptide chain from the start codon to the stop codon. (B)</p> Signup and view all the answers

What triggers the release of the completed polypeptide during termination?

<p>Recognition of the stop codon. (D)</p> Signup and view all the answers

Which factor contributes to the accuracy of translation at the ribosome?

<p>The complementary base pairing between tRNA and codon. (C)</p> Signup and view all the answers

Flashcards

Genetic Code

The sequence of bases in mRNA molecules that determines the sequence of amino acids in proteins.

Codon

A sequence of three mRNA nucleotides that specifies a particular amino acid.

Transcription

The process of creating an RNA copy of a DNA gene.

Translation

The process of synthesizing a protein from an mRNA molecule.

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Central Dogma

The fundamental concept that genetic information flows from DNA to RNA to protein.

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Transcription Initiation

The beginning phase of gene expression where RNA polymerase binds to the promoter region of DNA to begin synthesizing RNA

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Transcription Elongation

The middle stage of transcription where RNA polymerase synthesizes a complementary RNA molecule from a DNA template

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Transcription Termination

The phase of transcription where RNA polymerase detaches from the DNA and releases the newly synthesized mRNA

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Eukaryotic Pre-mRNA Processing

Eukaryotic genes undergo additional steps, like splicing out non-coding regions (introns) and adding a 5' cap and 3' poly-A tail, before the mRNA can be used for translation

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Translation Machinery

The complex process of assembling proteins using mRNA, tRNA, ribosomes, and various factors. It's energy intensive

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tRNA structure

tRNA is a folded single strand of RNA with specific sites for amino acid attachment and codon recognition.

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Amino acid attachment site

The 3' end of tRNA where an amino acid binds during protein synthesis.

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Anticodon

A three-nucleotide sequence on tRNA that is complementary to a codon on mRNA.

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tRNA function

tRNA carries specific amino acids to the ribosome during protein synthesis, ensuring the correct amino acid sequence.

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mRNA codon

Three-nucleotide sequence in mRNA that codes for a specific amino acid.

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tRNA molecule variety

Different tRNA molecules exist for every amino acid.

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tRNA role in protein synthesis

tRNA brings amino acids to the ribosome, where they're linked together to form proteins.

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Structure-function relationship (tRNA)

The specific 3-D shape of tRNA allows it to perform its crucial role in protein synthesis, matching amino acids to mRNA codons.

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Ribosome Structure

Ribosomes are made of proteins and ribosomal RNA (rRNA). They have three sites (P, A, and E) for transfer RNA (tRNA).

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tRNA Binding Sites

Ribosomes have three tRNA binding sites: P (growing polypeptide), A (next amino acid), and E (exit).

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Translation Initiation

mRNA, first tRNA, and ribosomal subunits join to start protein synthesis.

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Translation Elongation

Protein synthesis continues, adding amino acids from the start codon to the stop codon.

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Translation Termination

Protein synthesis stops at the stop codon and the complex breaks down.

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Ribosome Function

Ribosomes read mRNA instructions to assemble amino acids into proteins.

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mRNA Role

mRNA carries the genetic code from DNA to the ribosome.

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Start Codon

The codon (AUG) that signals the start of protein synthesis.

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Amino Acid Sequence

The order in which amino acids are linked together to form a protein.

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tRNA Anticodon

A three-base sequence on tRNA that is complementary to a codon on mRNA.

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Codon-Anticodon Pairing

The specific interaction between a codon on mRNA and an anticodon on tRNA, determining which amino acid is added to the growing polypeptide chain.

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Amino Acid Bond Formation

Peptide bond formation linking amino acids during protein synthesis.

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mRNA Codon

A three-base sequence on messenger RNA that codes for a particular amino acid.

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Genetic Code

The set of rules determining how codons are translated into amino acids during protein synthesis.

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tRNA Structure

Transfer RNA molecules have an anticodon loop and an amino acid attachment site.

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Protein Synthesis

The overall process of building a protein using information from mRNA.

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Ribosome Movement

The ribosome moves along the mRNA to read the genetic code and assemble the polypeptide chain.

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Multiple tRNAs

Two tRNAs can be at the ribosome at the same time, binding to different codons.

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Peptide Bond Formation

The formation of a bond between amino acids in a polypeptide chain.

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tRNA Exit (E Site)

The tRNA that has already contributed its amino acid to the chain releases and exits the ribosome using the E Site.

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Stop Codon Recognition

Translation terminates when a stop codon on mRNA is reached and a release factor binds.

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A site (Ribosome)

The place on the ribosome where a new aminoacyl-tRNA binds during protein synthesis.

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Amino Acid Arrival

The next amino acid linked to its tRNA approaches and binds to the ribosome in the A site.

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Elongation Cycle

The process of adding amino acids one by one to the growing polypeptide chain.

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Study Notes

DNA and Genes

  • DNA information is in specific nucleotide sequences.
  • Genes control protein synthesis, linking genotype to phenotype.
  • Proteins affect traits.

Gene Expression

  • Gene expression is the process where DNA directs protein synthesis.
  • It involves two stages: transcription and translation.
  • RNA is an intermediary between genes and proteins.

Transcription

  • DNA has regions: a promoter (start signal) and a terminator (end signal).
  • The transcribed region has the amino acid sequence information.
  • Regulatory sequences affect transcription rate, regulating protein binding.
  • mRNA is synthesized 5' to 3', using the template strand of DNA, with uracil replacing thymine.

Genetic Code

  • The genetic code is the sequence of bases in mRNA (triplets).
  • Codon groups are read for specific amino acids.
  • Characteristics of the genetic code: Linear triplet, 64 possible codons (61 sense, 3 stop), unambiguous, redundant, non-overlapping coding sequences.

mRNA and tRNA

  • mRNA carries the genetic code as codons (three RNA nucleotides).
  • tRNA carries amino acids, recognizing mRNA codons with anticodons.

The Genetic Code Table

  • Shows which codons specify which amino acids.
  • Contains 64 codons.
  • Includes start (AUG) and stop codons (UAA, UAG, UGA).

mRNA in Translation

  • mRNA has a ribosomal-binding site.
  • The primary start codon is AUG.
  • Polypeptides usually contain many amino acids.
  • Stop codons signify the end of the polypeptide sequence (UAA, UAG, or UGA).

Ribosomes in Translation

  • Ribosomes are made of ribosomal RNA (rRNA) and proteins.
  • Ribosomes have P, A, and E sites for tRNA binding and polypeptide synthesis.

Stages of Translation

  • Initiation: mRNA, first tRNA, and ribosome subunits combine.
  • Elongation: Polypeptide synthesis from the start to stop codon.
  • Termination: The complex disassembles at the stop codon, releasing polypeptide, and the mRNA.

RNA Processing in Eukaryotes

  • Bacterial mRNA is directly translated into protein.
  • Eukaryotic pre-mRNA undergoes processing to mRNA via splicing, capping, and poly A tail addition.
  • Introns are non-coding sequences removed, while exons are coding sequences.

Splicing

  • Spliceosome (snRNPs, small nuclear RNAs, and proteins) remove introns.
  • Alternative splicing gives one gene multiple protein products.
  • Exon shuffling allows combination of exons from different genes.

RNA Processing Additional Steps

  • Capping: Added to the 5' end of mRNA preventing degradation and ribosome binding.
  • Poly A tail: Added to the 3' end enhancing mRNA stability.

Transcription in Eukaryotes

  • Eukaryotic promoters have TATA boxes.
  • Transcription factors aid recognition and binding of RNA polymerase.
  • Initiation complex assembles at the promoter.

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