Podcast
Questions and Answers
What does a 99.9% probability of paternity indicate?
What does a 99.9% probability of paternity indicate?
- There is insufficient evidence regarding paternity.
- The man is definitely not the father.
- The man is likely the father.
- The man is the father. (correct)
Which of the following is NOT a consideration in the ethical use of paternity testing?
Which of the following is NOT a consideration in the ethical use of paternity testing?
- Financial compensation for the testing (correct)
- Informed consent from all parties
- Confidentiality of test results
- The right to privacy
Before DNA testing, which of the following methods was used to assess paternity?
Before DNA testing, which of the following methods was used to assess paternity?
- Psychological evaluations
- Physical resemblance assessment
- X-ray analysis
- Blood group testing (correct)
How has DNA testing impacted family law?
How has DNA testing impacted family law?
What is the primary reason for maintaining confidentiality of paternity test results?
What is the primary reason for maintaining confidentiality of paternity test results?
What probability of paternity can be attributed to HLA typing alone?
What probability of paternity can be attributed to HLA typing alone?
What is a significant limitation of using polymorphic enzymes for paternity testing?
What is a significant limitation of using polymorphic enzymes for paternity testing?
Which technique is most commonly used for modern paternity testing due to its high accuracy?
Which technique is most commonly used for modern paternity testing due to its high accuracy?
What method is considered the most non-invasive for DNA collection?
What method is considered the most non-invasive for DNA collection?
Which of the following statements about Y-Chromosome Testing is true?
Which of the following statements about Y-Chromosome Testing is true?
What is a primary use of mitochondrial DNA (mtDNA) testing?
What is a primary use of mitochondrial DNA (mtDNA) testing?
What major drawback does HLA typing possess in comparison to other testing methods?
What major drawback does HLA typing possess in comparison to other testing methods?
What is the primary purpose of paternity testing?
What is the primary purpose of paternity testing?
Which of the following is NOT a scenario where paternity testing might be utilized?
Which of the following is NOT a scenario where paternity testing might be utilized?
Which aspect of paternity testing gives it low accuracy on its own?
Which aspect of paternity testing gives it low accuracy on its own?
Before modern genetic testing, which methods were commonly used to resolve disputes regarding paternity?
Before modern genetic testing, which methods were commonly used to resolve disputes regarding paternity?
What is a characteristic of Non-Invasive Prenatal Paternity Testing (NIPT)?
What is a characteristic of Non-Invasive Prenatal Paternity Testing (NIPT)?
According to Mendel's law of inheritance, what factors can a legitimate child inherit from parents?
According to Mendel's law of inheritance, what factors can a legitimate child inherit from parents?
What blood group is considered the commonest based on the given inheritance factors?
What blood group is considered the commonest based on the given inheritance factors?
When a homozygous Rh -ve woman marries a homozygous Rh +ve man, what blood group will their child likely have?
When a homozygous Rh -ve woman marries a homozygous Rh +ve man, what blood group will their child likely have?
What method carries a higher risk to the fetus during prenatal testing?
What method carries a higher risk to the fetus during prenatal testing?
Which of the following statements best captures the importance of informed consent in paternity testing?
Which of the following statements best captures the importance of informed consent in paternity testing?
Which of these methods is NOT categorized under Pre-DNA Testing Methods?
Which of these methods is NOT categorized under Pre-DNA Testing Methods?
What remains central to the use of paternity testing in society, particularly concerning the rights of individuals?
What remains central to the use of paternity testing in society, particularly concerning the rights of individuals?
What significant advantage does modern DNA testing provide over previous paternity assessment methods?
What significant advantage does modern DNA testing provide over previous paternity assessment methods?
Which of the following best describes the primary role of paternity testing in legal contexts?
Which of the following best describes the primary role of paternity testing in legal contexts?
What is a major advantage of using STR (Short Tandem Repeat) Analysis in paternity testing?
What is a major advantage of using STR (Short Tandem Repeat) Analysis in paternity testing?
Which scenario would most likely require court-ordered paternity testing?
Which scenario would most likely require court-ordered paternity testing?
In which scenario is mitochondrial DNA (mtDNA) testing most applicable?
In which scenario is mitochondrial DNA (mtDNA) testing most applicable?
What was a major limitation of early paternity assessment methods?
What was a major limitation of early paternity assessment methods?
Which method is considered the most conclusive for resolving paternity disputes?
Which method is considered the most conclusive for resolving paternity disputes?
What does NIPT stand for and what is its primary method of operation?
What does NIPT stand for and what is its primary method of operation?
What is a key limitation of HLA typing in paternity testing?
What is a key limitation of HLA typing in paternity testing?
According to Mendel's law, which blood group combinations indicate an ABO group inheritance?
According to Mendel's law, which blood group combinations indicate an ABO group inheritance?
What common factor does blood group inheritance between a heterozygous Rh +ve couple lead to?
What common factor does blood group inheritance between a heterozygous Rh +ve couple lead to?
Which of the following is NOT typically a consideration when using polymorphic enzymes for paternity testing?
Which of the following is NOT typically a consideration when using polymorphic enzymes for paternity testing?
What type of prenatal testing poses a risk to the fetus?
What type of prenatal testing poses a risk to the fetus?
Which factor does a child inherit from parents, based on Mendel's law?
Which factor does a child inherit from parents, based on Mendel's law?
Which paternity testing technique is considered difficult and costly but provides high accuracy?
Which paternity testing technique is considered difficult and costly but provides high accuracy?
What is the primary purpose of paternity testing in immigration cases?
What is the primary purpose of paternity testing in immigration cases?
When analyzing antigens on the surface of white blood cells, which testing method is being utilized?
When analyzing antigens on the surface of white blood cells, which testing method is being utilized?
Inherited blood group combinations can lead to which of the following scenarios?
Inherited blood group combinations can lead to which of the following scenarios?
Explain the significance of the 0% probability in paternity testing.
Explain the significance of the 0% probability in paternity testing.
What ethical considerations must be addressed when conducting paternity tests?
What ethical considerations must be addressed when conducting paternity tests?
How did pre-DNA testing methods impact the legal context of paternity disputes?
How did pre-DNA testing methods impact the legal context of paternity disputes?
What role does confidentiality play in the context of paternity testing results?
What role does confidentiality play in the context of paternity testing results?
Discuss the impact of modern DNA testing on family law.
Discuss the impact of modern DNA testing on family law.
What are the potential blood types of a child from two heterozygous Rh +ve parents (Dd)?
What are the potential blood types of a child from two heterozygous Rh +ve parents (Dd)?
What does HLA typing analyze and what is its probability of paternity alone?
What does HLA typing analyze and what is its probability of paternity alone?
Why are polymorphic enzyme assays considered to have moderate exclusion power?
Why are polymorphic enzyme assays considered to have moderate exclusion power?
What is the primary advantage of using PCR in paternity testing?
What is the primary advantage of using PCR in paternity testing?
In what scenario is Y-Chromosome Testing particularly useful?
In what scenario is Y-Chromosome Testing particularly useful?
What makes STR (Short Tandem Repeat) testing preferred in modern paternity testing?
What makes STR (Short Tandem Repeat) testing preferred in modern paternity testing?
Describe a limitation of using buccal swabs for DNA collection.
Describe a limitation of using buccal swabs for DNA collection.
How does mitochondrial DNA (mtDNA) testing contribute to understanding paternity?
How does mitochondrial DNA (mtDNA) testing contribute to understanding paternity?
What are two possible scenarios that could lead to disputes over paternity?
What are two possible scenarios that could lead to disputes over paternity?
Describe the primary difference between Non-Invasive Prenatal Testing (NIPT) and invasive prenatal testing methods.
Describe the primary difference between Non-Invasive Prenatal Testing (NIPT) and invasive prenatal testing methods.
According to Mendel's law of inheritance, which two blood groups are dominant and which one is recessive?
According to Mendel's law of inheritance, which two blood groups are dominant and which one is recessive?
What is the inheritance pattern for a child born to a homozygous Rh -ve woman and a homozygous Rh +ve man?
What is the inheritance pattern for a child born to a homozygous Rh -ve woman and a homozygous Rh +ve man?
What were the major limitations of blood typing compared to modern genetic testing for paternity disputes?
What were the major limitations of blood typing compared to modern genetic testing for paternity disputes?
List two uses of paternity testing beyond establishing familial relationships.
List two uses of paternity testing beyond establishing familial relationships.
What is a significant risk factor associated with invasive prenatal testing methods?
What is a significant risk factor associated with invasive prenatal testing methods?
Name one reason why paternity testing might be court-ordered.
Name one reason why paternity testing might be court-ordered.
What is the main ethical consideration highlighted in the use of paternity testing regarding the individuals involved?
What is the main ethical consideration highlighted in the use of paternity testing regarding the individuals involved?
How did the introduction of DNA testing improve the process of determining paternity compared to pre-DNA methods?
How did the introduction of DNA testing improve the process of determining paternity compared to pre-DNA methods?
What does a 99.9% probability of paternity typically indicate about the alleged father's biological relationship to the child?
What does a 99.9% probability of paternity typically indicate about the alleged father's biological relationship to the child?
What was one limitation of pre-DNA testing methods that made them less effective than modern DNA testing?
What was one limitation of pre-DNA testing methods that made them less effective than modern DNA testing?
In what ways does the modern paternity testing landscape influence the legal rights of the child?
In what ways does the modern paternity testing landscape influence the legal rights of the child?
What are two primary uses of Y-Chromosome Testing?
What are two primary uses of Y-Chromosome Testing?
What is one limitation of using polymorphic enzymes for paternity testing?
What is one limitation of using polymorphic enzymes for paternity testing?
How does HLA typing differ from traditional blood type testing in terms of paternity probability?
How does HLA typing differ from traditional blood type testing in terms of paternity probability?
What is a significant advantage of STR analysis in modern paternity testing?
What is a significant advantage of STR analysis in modern paternity testing?
What sample collection method is preferred for non-invasive DNA testing?
What sample collection method is preferred for non-invasive DNA testing?
Describe a main characteristic of mitochondrial DNA (mtDNA) testing.
Describe a main characteristic of mitochondrial DNA (mtDNA) testing.
What is the probability of paternity when using PCR and STR analysis together?
What is the probability of paternity when using PCR and STR analysis together?
What is one drawback of HLA typing besides its cost?
What is one drawback of HLA typing besides its cost?
What genetic testing method is used to analyze fetal DNA during pregnancy without posing risks to the fetus?
What genetic testing method is used to analyze fetal DNA during pregnancy without posing risks to the fetus?
Explain why blood group O is considered recessive in inheritance.
Explain why blood group O is considered recessive in inheritance.
In a situation where a father denies paternity, what type of testing may be ordered by the court?
In a situation where a father denies paternity, what type of testing may be ordered by the court?
What is a major drawback of invasive prenatal testing methods like amniocentesis?
What is a major drawback of invasive prenatal testing methods like amniocentesis?
According to Mendel's law, how many inheritable factors can a legitimate child receive from their parents?
According to Mendel's law, how many inheritable factors can a legitimate child receive from their parents?
What testing method uses the mother's blood to analyze fetal DNA fragments in non-invasive procedures?
What testing method uses the mother's blood to analyze fetal DNA fragments in non-invasive procedures?
What are the potential blood types for a child born to a heterozygous Rh +ve father and a homozygous Rh -ve mother?
What are the potential blood types for a child born to a heterozygous Rh +ve father and a homozygous Rh -ve mother?
Name one application of paternity testing beyond determining parentage.
Name one application of paternity testing beyond determining parentage.
Flashcards
Disputed Paternity
Disputed Paternity
Determining biological parentage using genetic tests, often in cases of dispute or uncertainty
Paternity Testing
Paternity Testing
Use of genetic analysis to identify the biological father of a child
Blood Typing
Blood Typing
A method for determining blood groups (e.g., ABO, Rh), which can be used in paternity cases, but is not fully conclusive on its own.
ABO Blood Group System
ABO Blood Group System
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Rh Factor
Rh Factor
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Non-Invasive Prenatal Paternity Testing (NIPT)
Non-Invasive Prenatal Paternity Testing (NIPT)
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Invasive Prenatal Testing
Invasive Prenatal Testing
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Prenatal Testing
Prenatal Testing
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Postnatal Testing
Postnatal Testing
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Mendel's Law of Inheritance
Mendel's Law of Inheritance
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Blood Typing
Blood Typing
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Blood Typing Exclusion
Blood Typing Exclusion
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Polymorphic Enzymes
Polymorphic Enzymes
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HLA Typing
HLA Typing
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PCR (Polymerase Chain Reaction)
PCR (Polymerase Chain Reaction)
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STR (Short Tandem Repeat) Analysis
STR (Short Tandem Repeat) Analysis
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Y-Chromosome Testing
Y-Chromosome Testing
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Mitochondrial DNA (mtDNA) Testing
Mitochondrial DNA (mtDNA) Testing
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Buccal Swabs
Buccal Swabs
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Blood Samples
Blood Samples
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DNA Extraction
DNA Extraction
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Paternity Testing
Paternity Testing
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Paternity Testing
Paternity Testing
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DNA Profile
DNA Profile
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Inclusion (paternity)
Inclusion (paternity)
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Exclusion (paternity)
Exclusion (paternity)
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Informed Consent
Informed Consent
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Privacy Rights (paternity tests)
Privacy Rights (paternity tests)
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Pre-DNA Paternity Methods
Pre-DNA Paternity Methods
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Ethical Considerations (paternity testing)
Ethical Considerations (paternity testing)
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Disputed Paternity
Disputed Paternity
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Paternity Testing
Paternity Testing
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Blood Typing
Blood Typing
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ABO Blood Group
ABO Blood Group
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Rh Factor
Rh Factor
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Non-Invasive Prenatal Testing (NIPT)
Non-Invasive Prenatal Testing (NIPT)
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Invasive Prenatal Testing
Invasive Prenatal Testing
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Prenatal Testing
Prenatal Testing
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Postnatal Testing
Postnatal Testing
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Mendel's Law of Inheritance
Mendel's Law of Inheritance
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Blood Typing Exclusion
Blood Typing Exclusion
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DNA Profile
DNA Profile
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Paternity Testing Methods
Paternity Testing Methods
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Blood Typing Exclusion
Blood Typing Exclusion
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Polymorphic Enzymes
Polymorphic Enzymes
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HLA Typing
HLA Typing
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PCR
PCR
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STR Analysis
STR Analysis
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Y-Chromosome Testing
Y-Chromosome Testing
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Mitochondrial DNA (mtDNA)
Mitochondrial DNA (mtDNA)
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Buccal Swabs
Buccal Swabs
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Blood Sample in Paternity Testing
Blood Sample in Paternity Testing
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DNA Extraction
DNA Extraction
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Paternity Testing
Paternity Testing
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DNA Profile
DNA Profile
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Inclusion (Paternity)
Inclusion (Paternity)
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Exclusion (Paternity)
Exclusion (Paternity)
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Pre-DNA Paternity Methods
Pre-DNA Paternity Methods
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Informed Consent
Informed Consent
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Privacy Rights (Paternity Tests)
Privacy Rights (Paternity Tests)
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Ethical Considerations
Ethical Considerations
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Blood Typing
Blood Typing
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Disputed Paternity
Disputed Paternity
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Disputed Paternity
Disputed Paternity
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Paternity Testing
Paternity Testing
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ABO Blood Group
ABO Blood Group
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Rh Factor
Rh Factor
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Non-Invasive Prenatal Testing (NIPT)
Non-Invasive Prenatal Testing (NIPT)
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Invasive Prenatal Testing
Invasive Prenatal Testing
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Prenatal Testing
Prenatal Testing
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Postnatal Testing
Postnatal Testing
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Mendel's Law of Inheritance
Mendel's Law of Inheritance
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Blood Typing Exclusion
Blood Typing Exclusion
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DNA Profile
DNA Profile
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Inclusion (Paternity)
Inclusion (Paternity)
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Exclusion (Paternity)
Exclusion (Paternity)
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Heterozygous Rh+ve woman
Heterozygous Rh+ve woman
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Heterozygous Rh+ve man
Heterozygous Rh+ve man
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Blood Typing Exclusion
Blood Typing Exclusion
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Polymorphic Enzymes
Polymorphic Enzymes
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HLA Typing
HLA Typing
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PCR (Polymerase Chain Reaction)
PCR (Polymerase Chain Reaction)
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STR Analysis
STR Analysis
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Y-Chromosome Testing
Y-Chromosome Testing
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Mitochondrial DNA (mtDNA) Testing
Mitochondrial DNA (mtDNA) Testing
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Buccal Swabs
Buccal Swabs
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Blood Sample in Paternity Testing
Blood Sample in Paternity Testing
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Paternity Testing
Paternity Testing
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DNA Profile
DNA Profile
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Inclusion (Paternity)
Inclusion (Paternity)
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Exclusion (Paternity)
Exclusion (Paternity)
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Pre-DNA Paternity Methods
Pre-DNA Paternity Methods
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Informed Consent
Informed Consent
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Privacy Rights (Paternity Tests)
Privacy Rights (Paternity Tests)
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Ethical Considerations (Paternity Testing)
Ethical Considerations (Paternity Testing)
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Blood Typing
Blood Typing
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Disputed Paternity
Disputed Paternity
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Disputed Paternity
Disputed Paternity
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Paternity Testing
Paternity Testing
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ABO Blood Group
ABO Blood Group
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Rh Factor
Rh Factor
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Non-Invasive Prenatal Testing (NIPT)
Non-Invasive Prenatal Testing (NIPT)
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Invasive Prenatal Testing
Invasive Prenatal Testing
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Prenatal Testing
Prenatal Testing
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Postnatal Testing
Postnatal Testing
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Mendel's Law of inheritance
Mendel's Law of inheritance
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Blood Typing Exclusion
Blood Typing Exclusion
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DNA Profile
DNA Profile
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Inclusion (Paternity)
Inclusion (Paternity)
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Exclusion (Paternity)
Exclusion (Paternity)
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Heterozygous Rh+ve woman (Dd)
Heterozygous Rh+ve woman (Dd)
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Paternity Testing
Paternity Testing
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DNA Profile
DNA Profile
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Heterozygous Rh+ve man (Dd)
Heterozygous Rh+ve man (Dd)
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Inclusion (Paternity)
Inclusion (Paternity)
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Blood Typing
Blood Typing
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Blood Typing Exclusion
Blood Typing Exclusion
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Exclusion (Paternity)
Exclusion (Paternity)
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Pre-DNA Paternity Methods
Pre-DNA Paternity Methods
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Polymorphic Enzymes
Polymorphic Enzymes
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HLA Typing
HLA Typing
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Informed Consent
Informed Consent
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Privacy Rights
Privacy Rights
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PCR (Polymerase Chain Reaction)
PCR (Polymerase Chain Reaction)
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STR Analysis
STR Analysis
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Ethical Considerations (Paternity Testing)
Ethical Considerations (Paternity Testing)
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Blood Typing
Blood Typing
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Y-Chromosome Testing
Y-Chromosome Testing
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Disputed Paternity
Disputed Paternity
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Mitochondrial DNA (mtDNA) Testing
Mitochondrial DNA (mtDNA) Testing
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Paternity Testing Methods
Paternity Testing Methods
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Buccal Swabs
Buccal Swabs
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Blood Sample in Paternity Testing
Blood Sample in Paternity Testing
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DNA Profile
DNA Profile
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Paternity Testing
Paternity Testing
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Study Notes
Disputed Paternity
-
Definition of Paternity: Parenthood; paternity testing uses genetic methods to determine relationships, often between parents and children.
-
Methods of Disputed Paternity (Pre-DNA Era): Witness testimony, cultural norms, and early scientific methods like blood typing, which were less accurate and often inconclusive.
Human Genetically Controlled Markers Systems
- Pre-DNA Era Markers:
- Blood groups (ABO system, Rh factor)
- Enzyme groups
- Protein groups
- HLA (Human Leukocytes Antigen)
- DNA Fingerprints
Types of Paternity Testing
-
Prenatal Testing:
- Non-Invasive: Uses the mother's blood to analyze fetal DNA fragments; no risk to the fetus.
- Invasive: Amniocentesis and chorionic villus sampling (CVS) used to collect fetal DNA, carries a risk to the fetus.
-
Postnatal Testing: Performed after birth.
Inheritance of Blood Groups (ABO System)
- Mendel's Law: Three inheritable factors: A and B are dominant, O is recessive.
- Inheritance Pattern: Children inherit one factor from each parent, determining their blood type.
- Blood Group Combinations:
- AB (rarest)
- O (most common)
- AO/BO - A or B group
Rh Factor
- Inheritance: Inherited by two genes: D (dominant) and d (recessive).
- Homozygous Rh-ve Woman and Homozygous Rh+ve Man: Child will be Rh+.
- Heterozygous Rh+ve Woman and Heterozygous Rh+ve Man: Child may be Rh+ or Rh-.
Advantages and Disadvantages of Blood Typing
- Advantages: Easy, rapid, cheap, 100% exclusion.
- Disadvantages: Inaccurate for conclusive proof, multiple matches possible (many people can share the same blood type).
Enzyme Groups
- Definition: Polymorphic enzymes (e.g., lactate dehydrogenase) primarily in red blood cells, tested through electrophoresis.
- Advantages: Rapid, non-expensive.
- Disadvantages: Non-conclusive alone.
3-Protein Groups
- Definition: Analyzes inherited proteins and enzymes (e.g., haptoglobins, immunoglobulins).
- Advantages: Moderate exclusion power.
- Disadvantages: Could not definitively prove paternity; low accuracy.
HLA (Human Leukocytes Antigen)
- Definition: Analyzes antigens on white blood cells.
- Advantages: Alone, 98% probability of paternity.
- Disadvantages: Difficult, expensive technique.
DNA Typing (DNA Profiling)
- PCR (Polymerase Chain Reaction): Amplifies small DNA samples.
- STR (Short Tandem Repeat) Analysis: Compares short DNA sequences at specific locations. Highly accurate and the most common method for modern paternity testing.
- Y-Chromosome Testing: For testing relationships between males; Y-chromosome passes from father to son.
- Mitochondrial DNA (mtDNA) Testing: Primarily for maternal lineage; can assist in complex paternity cases
DNA Testing Process
- Sample Collection: Buccal (cheek) swabs, blood samples, hair follicles, other tissues.
- Analysis (STR): Analyzing DNA sequences.
- Report Generation: Reporting results.
Probability of Paternity
- Results expressed as a probability. 99.9% probability indicates inclusion (the man is the father). 0% indicates exclusion.
Paternity Testing Inclusion/Exclusion
- Inclusion: DNA profile of alleged father matches the child's, confirming biological relationship.
- Exclusion: DNA profile of alleged father does not match, excluding that person as the biological father.
Ethical Considerations
- Informed consent required for all parties.
- Privacy and confidentiality of test results must be maintained.
- Rights of the child central to the use of paternity testing.
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