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What is the purpose of the disk-diffusion assay method?
What is the purpose of the disk-diffusion assay method?
- To measure the diameter of the agar plate
- To assess the effectiveness of chemicals on inhibiting microbial growth (correct)
- To determine the growth rate of bacteria
- To study the diffusion of agar into filter paper disks
In the disk-diffusion assay, what contributes to the size of the zone of inhibition?
In the disk-diffusion assay, what contributes to the size of the zone of inhibition?
- The number of bacteria present on the agar plate
- The presence of zones of growth
- The ability of the chemical agent to diffuse in the agar (correct)
- The color of the filter paper disks
How are zones of inhibition observed in a disk-diffusion assay?
How are zones of inhibition observed in a disk-diffusion assay?
- As colored regions on the agar plate
- As cloudy patches on the filter paper disks
- As areas with increased microbial growth
- As cleared areas or halos around the paper disks (correct)
What is typically associated with larger zones of inhibition in a disk-diffusion assay?
What is typically associated with larger zones of inhibition in a disk-diffusion assay?
How is the diameter of the zone of inhibition measured in a disk-diffusion assay?
How is the diameter of the zone of inhibition measured in a disk-diffusion assay?
What is the purpose of applying different chemicals to sterile filter paper disks in the disk-diffusion assay?
What is the purpose of applying different chemicals to sterile filter paper disks in the disk-diffusion assay?
In the disk-diffusion assay, what contributes to the appearance of zones of inhibition?
In the disk-diffusion assay, what contributes to the appearance of zones of inhibition?
How does the size of the zone of inhibition typically correlate with the effectiveness of a chemical agent in the disk-diffusion assay?
How does the size of the zone of inhibition typically correlate with the effectiveness of a chemical agent in the disk-diffusion assay?
What does the diameter measurement of the zone of inhibition represent in a disk-diffusion assay?
What does the diameter measurement of the zone of inhibition represent in a disk-diffusion assay?
What effect do water-soluble agents have on the size of the zone of inhibition in a disk-diffusion assay?
What effect do water-soluble agents have on the size of the zone of inhibition in a disk-diffusion assay?
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Study Notes
Disk-Diffusion Assay
- The disk-diffusion assay involves applying different chemicals (e.g., disinfectants or antiseptics) to individual sterile filter paper disks.
- The disks are placed on an agar plate that has been inoculated with a bacterium of interest and incubated for 24-72 hours.
- During incubation, chemicals diffuse into the agar, and zones of inhibition of microbial growth appear as cleared areas or halos around the paper disk.
- The size of the zone of inhibition is influenced by factors such as whether the agent is water soluble and able to diffuse in the agar.
- Larger zones of inhibition typically correlate to increased inhibition effectiveness of the chemical agent.
- The diameter of the zone of inhibition is measured in millimeters using a ruler to quantify the effectiveness of the chemical agent.
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