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Questions and Answers
What is the main purpose of mass spectrometry?
What is the main purpose of mass spectrometry?
- To analyze neuronal cells exposed to toxicity
- To convert molecules into gas-phase ions
- To measure the mass of molecules accurately (correct)
- To identify post-translational modifications
Which of the following fields can benefit from mass spectrometry?
Which of the following fields can benefit from mass spectrometry?
- Astrophysics
- Biochemistry research (correct)
- Sociology
- Agriculture
What happens to a molecule during the mass spectrometry technique?
What happens to a molecule during the mass spectrometry technique?
- It is deflected by a magnetic field (correct)
- It is converted into a liquid-phase ion
- It is analyzed through enzymatic cleavage
- It is separated by electrophoresis
How can post-translational modifications be identified using mass spectrometry?
How can post-translational modifications be identified using mass spectrometry?
In what field can mass spectrometry be used to differentiate neuronal cells exposed to toxicity?
In what field can mass spectrometry be used to differentiate neuronal cells exposed to toxicity?
What is a good practice when using mass spectrometry in follow-up experiments?
What is a good practice when using mass spectrometry in follow-up experiments?
What are some types of ionization methods available in mass spectrometry?
What are some types of ionization methods available in mass spectrometry?
What are some methods for analyzing proteins separated by electrophoresis?
What are some methods for analyzing proteins separated by electrophoresis?
What can image analysis software be used for in analyzing proteins separated by electrophoresis?
What can image analysis software be used for in analyzing proteins separated by electrophoresis?
What is peptide mass fingerprinting?
What is peptide mass fingerprinting?
What areas of research has mass spectrometry contributed significantly to?
What areas of research has mass spectrometry contributed significantly to?
What is the size range of molecules that can be accurately measured using mass spectrometry?
What is the size range of molecules that can be accurately measured using mass spectrometry?
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Study Notes
- Mass spectrometry is used to measure the mass of molecules ranging from large proteins to small sugars accurately.
- It has many potential applications in scientific research, including drug discovery, biochemistry research, clinical chemistry, food safety, and environmental research.
- The technique involves converting the molecule into a gas-phase ion, which is then deflected by a magnetic field and accelerated towards negative plates with speed depending on mass.
- Proteins can be analyzed through enzymatic cleavage and electrophoresis, and post-translational modifications can be identified.
- Mass spectrometry can differentiate neuronal cells exposed to organophosphate toxicity by analyzing biomarkers and changes in protein expression levels.
- Alternative approaches should be used in follow-up experiments for good practice.
- Different types of ionization methods are available, such as electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI).
- Proteins separated by electrophoresis can be analyzed through SDS-PAGE or 2D-PAGE, and image analysis software can be used to highlight spots showing more than two-fold changes.
- Peptide mass fingerprinting is a method that is effective in identifying proteins from mass spectrometry and liquid chromatography.
- Mass spectrometry has contributed significantly to the study and discovery of new drugs, studies into protein adduct formation, metabolism, and other vital areas of research.
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