Podcast
Questions and Answers
What is the formula for calculating dilution factor (DF)?
What is the formula for calculating dilution factor (DF)?
- DF = Final volume - Solute volume
- DF = Solute volume * Final volume
- DF = Solute volume / Final volume
- DF = Final volume / Solute volume (correct)
In the Bradford assay, at what pH does the Coomassie Brilliant Blue G-250 dye have a blue color?
In the Bradford assay, at what pH does the Coomassie Brilliant Blue G-250 dye have a blue color?
- 1 < pH < 2
- pH > 2 (correct)
- pH = 7
- pH < 1
What is the wavelength of maximum absorption of the Coomassie Brilliant Blue G-250 dye when it is not yet bound to protein?
What is the wavelength of maximum absorption of the Coomassie Brilliant Blue G-250 dye when it is not yet bound to protein?
- 420 nm (correct)
- It is not specified in the text
- 650 nm
- 550 nm
What is the equation for the relationship between protein standard concentration (x) and absorbance (y) in the Bradford assay?
What is the equation for the relationship between protein standard concentration (x) and absorbance (y) in the Bradford assay?
If the absorbance (y) is 0.2137, what is the protein standard concentration (x) according to the Bradford assay equation?
If the absorbance (y) is 0.2137, what is the protein standard concentration (x) according to the Bradford assay equation?
What is the absorbance for a protein standard concentration of 1.5 ug/uL according to the Bradford assay equation?
What is the absorbance for a protein standard concentration of 1.5 ug/uL according to the Bradford assay equation?
What is the function of the UV-visible spectrophotometer?
What is the function of the UV-visible spectrophotometer?
What happens if the absorbance of a protein sample is higher than the absorbance of the standard solution with the highest concentration?
What happens if the absorbance of a protein sample is higher than the absorbance of the standard solution with the highest concentration?
What is the minimum molecular weight required for proteins to be detected by the Bradford assay?
What is the minimum molecular weight required for proteins to be detected by the Bradford assay?
Why should absorbance be measured immediately after mixing the dye with the protein sample?
Why should absorbance be measured immediately after mixing the dye with the protein sample?
