Recent Lessons

Show all results for ""

Feature Overview

Ace your exams with our all-in-one platform for creating and sharing quizzes and tests.

Quizzes

Create quizzes and tests automatically from your content using AI.

Flashcards

Automatically turn your notes into digital flashcards.

Share, Export & Embed

Share with classmates or export to Excel and your learning management system.

Stats & Reporting

Auto-grading quizzes and tests with detailed stats and reports.

Mobile Apps

The smarter way to study – wherever you are.

Pricing

Search...

10 Questions

4 Views

Dilution Calculations and Preparation

Choose a study mode

Play Quiz

Study Flashcards

Spaced Repetition

Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the formula for calculating dilution factor (DF)?

DF = Final volume - Solute volume

DF = Solute volume * Final volume

DF = Solute volume / Final volume

DF = Final volume / Solute volume (correct)

In the Bradford assay, at what pH does the Coomassie Brilliant Blue G-250 dye have a blue color?

1 < pH < 2

pH > 2 (correct)

pH = 7

pH < 1

What is the wavelength of maximum absorption of the Coomassie Brilliant Blue G-250 dye when it is not yet bound to protein?

420 nm (correct)

It is not specified in the text

650 nm

550 nm

What is the equation for the relationship between protein standard concentration (x) and absorbance (y) in the Bradford assay?

y = 0.2869x - 0.0007 Signup and view all the answers

If the absorbance (y) is 0.2137, what is the protein standard concentration (x) according to the Bradford assay equation?

0.7208 ug/uL Signup and view all the answers

What is the absorbance for a protein standard concentration of 1.5 ug/uL according to the Bradford assay equation?

0.4645 Signup and view all the answers

What is the function of the UV-visible spectrophotometer?

To measure the absorbance or transmittance of light in the ultraviolet and visible range of the electromagnetic radiation spectrum Signup and view all the answers

What happens if the absorbance of a protein sample is higher than the absorbance of the standard solution with the highest concentration?

Another standard solution with a higher concentration should be prepared Signup and view all the answers

What is the minimum molecular weight required for proteins to be detected by the Bradford assay?

3000 Daltons Signup and view all the answers

Why should absorbance be measured immediately after mixing the dye with the protein sample?

To avoid precipitation of the dye-protein complex Signup and view all the answers