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Dilution Calculations and Preparation

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Questions and Answers

What is the formula for calculating dilution factor (DF)?

DF = Final volume - Solute volume

DF = Solute volume * Final volume

DF = Solute volume / Final volume

DF = Final volume / Solute volume (correct)

In the Bradford assay, at what pH does the Coomassie Brilliant Blue G-250 dye have a blue color?

1 < pH < 2

pH > 2 (correct)

pH = 7

pH < 1

What is the wavelength of maximum absorption of the Coomassie Brilliant Blue G-250 dye when it is not yet bound to protein?

420 nm (correct)

It is not specified in the text

650 nm

550 nm

What is the equation for the relationship between protein standard concentration (x) and absorbance (y) in the Bradford assay?

y = 0.2869x - 0.0007 Signup and view all the answers

If the absorbance (y) is 0.2137, what is the protein standard concentration (x) according to the Bradford assay equation?

0.7208 ug/uL Signup and view all the answers

What is the absorbance for a protein standard concentration of 1.5 ug/uL according to the Bradford assay equation?

0.4645 Signup and view all the answers

What is the function of the UV-visible spectrophotometer?

To measure the absorbance or transmittance of light in the ultraviolet and visible range of the electromagnetic radiation spectrum Signup and view all the answers

What happens if the absorbance of a protein sample is higher than the absorbance of the standard solution with the highest concentration?

Another standard solution with a higher concentration should be prepared Signup and view all the answers

What is the minimum molecular weight required for proteins to be detected by the Bradford assay?

3000 Daltons Signup and view all the answers

Why should absorbance be measured immediately after mixing the dye with the protein sample?

To avoid precipitation of the dye-protein complex Signup and view all the answers