18 Questions
What does a straight-line fit of the data for the average number of A's bound per macromolecule vs. total concentration of A indicate?
The macromolecule binds A molecules in a linear manner
Why does the experimental data indicate that [A]T,in > [A]T,out in all cases?
The macromolecule binds some A molecules
What does the Scatchard equation predict about the slope of the straight-line fit of the data?
The slope is equal to -K
In the context of the experiment, what does the term 'A' represent?
Free A molecules
How is the average number of A's bound per macromolecule calculated?
([A]in - [A]out) / [M]
What can be inferred from the fact that the total concentration of A on the side without the macromolecule is greater than that on the side with the macromolecule?
The macromolecule binds some A molecules
What does the quantity [M] represent in the given text?
Total concentration of macromolecule
In the context of ligand binding, what does the equilibrium constant K represent?
Binding constant for the ligand
What does the quantity $ u$ represent in the ligand binding equation?
Average number of ligands bound per macromolecule
If a macromolecule has N equivalent binding sites, what is the average number of sites bound per macromolecule as per the given text?
$\frac{NK[A]}{1 + K[A]}$
Which statement best describes the role of Kj in ligand binding at site j?
Binding constant for the ligand at site j
What does the equation $K[A]\nu = \sum\nu_j = \sum(1 + K[A]) = NK[A] (1 + K[A])$ represent in the ligand binding context?
Average number of ligands bound per macromolecule
What is the assumption that the Scatchard equation depends on?
Identical and independent binding sites
Which method is often used to evaluate the degree of cooperativity in a binding process?
Hill plot
What is the primary reason more advanced treatments are required if the assumption of identical and independent binding sites is violated?
To consider the Donnan effect
What is the condition for electrical neutrality in solutions inside and outside the membrane in the Donnan effect context?
[Na+]out = [Cl-]out
In the Donnan effect scenario, what does equation (6) yield when using the relationship from equations (2) and (5)?
[Cl-]in = c2/[Na+]in
What is implied by the charge on the macromolecule (zM) in the context of the Donnan effect situation?
Being independent of the ligands
Test your knowledge on measuring total concentrations of substances across a dialysis membrane at equilibrium. Analyze concentration values on different sides of the membrane and calculate the equilibrium constant based on the provided data.
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