Diagnostic Microbiology Quiz
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Questions and Answers

What is the primary reason for the prompt transport of specimens to the laboratory?

  • To preserve the viability of fastidious microorganisms. (correct)
  • To facilitate easier labeling of samples.
  • To ensure samples are available for additional testing.
  • To allow for more time in specimen collection.
  • What is the consequence of improper specimen transport methods?

  • Decreased need for laboratory examination.
  • Increased likelihood of microbial growth.
  • Accurate identification of microorganisms.
  • Contamination of the specimen. (correct)
  • Which of the following is NOT a reason for using sterile, leakproof containers for specimen transport?

  • Enhance the visibility of specimens during transport. (correct)
  • Avoid exposure to potentially hazardous materials.
  • Prevent loss of sample.
  • Prevent extrinsic contamination.
  • Which statement about stool samples is most accurate?

    <p>They must be sent to the laboratory in sterile containers immediately.</p> Signup and view all the answers

    What is the correct procedure for collecting a throat swab?

    <p>Remove the swab from the tube and swab the back of the throat without touching the mouth.</p> Signup and view all the answers

    How should skin scrapings be collected and stored?

    <p>Swab with sterile gauze, scrape the lesion, and keep in sterile containers.</p> Signup and view all the answers

    Study Notes

    Diagnostic Microbiology

    • Diagnostic microbiology aims to identify microorganisms in samples.
    • Samples are collected from humans, animals, and the environment.

    Specimen Collection

    • Specimens should be collected from the suspected site of infection.
    • Sterile tools are used to avoid contamination.
    • Samples should be labeled with patient information.

    Tools for Specimen Collection

    • Swabs, syringes, tubes, petridishes, surgical blades, tweezers, anaerobic transport tubes, and fecal transport systems.

    Clinical Specimen Types

    • Blood: Collected with a sterile syringe after cleaning the skin.
    • Urine: Collected midstream in sterile containers.
    • Stool and rectal swabs: Collected in sterile containers.
    • Throat swab: Swab the back of the throat, avoiding other parts of the mouth.
    • Mouth and vagina swabs: Collected from mucosal surfaces.
    • Skin scrapings: Scraped with a sterile surgical blade from the edge of a lesion.
    • Hairs: Plucked from the site of a lesion.
    • Nail: Affected nail and scrapings from the nail bed.
    • Other samples: Spinal fluid, sputum, exudate from sinuses and abscesses, sternal marrow, and biopsies.

    Specimen Transport

    • Prompt transport is crucial to maintain viability and prevent overgrowth of microorganisms.
    • Specimens should be transported in sterile, leakproof containers.
    • Virus isolation specimens should be transported in a medium and kept chilled.
    • Anaerobic cultures should be collected in vials with an oxygen-free atmosphere.

    Plant and Soil Specimens

    • Proper sampling is crucial for accurate plant disease diagnosis.
    • Plant samples should be fresh and labelled, with leaf samples stored in plastic bags with moist paper towels.
    • Root systems should be placed in a separate bag.
    • Soil samples should be collected from the root zone with some root matter.
    • Different patterns are used for sampling based on the location and disease.

    Soil Sampling Patterns

    • Star pattern: Five "x" markings within a star, used for damaged areas.
    • Circle pattern: "x" markings on the perimeter of a circle, used for sampling a shrub.
    • Zig-zag pattern: Checkered pattern with "x" markings within a rectangle, used for fallow fields.

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    Description

    Test your knowledge on the essentials of diagnostic microbiology, including specimen collection techniques, tools used, and various clinical specimen types. This quiz covers the critical practices needed to identify microorganisms from different sources.

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