Microbiology: Specimen Collection and Diagnosis

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Questions and Answers

Which of the following is NOT a crucial step in the proper diagnosis of an infection?

  • Reviewing the patient's medical history
  • Performing a physical examination
  • Evaluating the patient's signs and symptoms
  • Ordering non-specific laboratory tests (correct)

Collecting a specimen after antimicrobial therapy has begun is the most effective approach for accurate pathogen identification.

False (B)

Why is proper transport of a specimen to the laboratory important?

To maintain specimen integrity and prevent degradation or contamination.

According to the content, the _ stage of a disease is the most opportune time to collect specimens.

<p>acute</p> Signup and view all the answers

Match each clinical specimen with a potential source of that specimen:

<p>Blood = Vein Feces = Digestive tract Urine = Bladder Sputum = Lungs</p> Signup and view all the answers

Which of the following outcomes is LEAST likely to result from improper specimen collection?

<p>Accurate identification of the causative agent (D)</p> Signup and view all the answers

A specimen container does not require a label if it is delivered to the lab immediately after collection.

<p>False (B)</p> Signup and view all the answers

What is the primary goal of obtaining a high-quality specimen?

<p>To achieve accurate and clinically relevant laboratory results.</p> Signup and view all the answers

According to the content, a pathologist is a physician with extensive training in _.

<p>pathology</p> Signup and view all the answers

Match each specimen type with the most appropriate container type for collection:

<p>Blood = Sterile tube with anticoagulant Urine = Sterile urine container Swab = Sterile transport swab Sputum = Sterile sputum container</p> Signup and view all the answers

Which of the following best describes 'anatomical pathology'?

<p>The performance of autopsies and examination of diseased organs (A)</p> Signup and view all the answers

Clinical pathology primarily deals with autopsies and examining diseased organs.

<p>False (B)</p> Signup and view all the answers

Why is it important to handle hazardous specimens with extra care?

<p>To avoid contamination of the courier, patients, and health care professionals.</p> Signup and view all the answers

According to the content, a specimen should be collected from the site where the suspected _ is most likely to be found.

<p>pathogen</p> Signup and view all the answers

Match each type of specimen with the appropriate method of collection listed below:

<p>Scotch tape prep = Pressing tape against skin to collect surface parasites Blood Draw = Venipuncture Urine sample = Clean catch midstream Throat Swab = Swabbing tonsils and posterior pharynx</p> Signup and view all the answers

Which of the following is the BEST reason for placing a specimen in a sterile container?

<p>To present contamination of the specimen (A)</p> Signup and view all the answers

Keeping a specimen cold is always the best way to preserve it until it reaches the lab.

<p>False (B)</p> Signup and view all the answers

What is "clinical pathology?"

<p>Clinical Pathology consists of blood bank, clinical microscopy, hematology, and the immunology lab.</p> Signup and view all the answers

According to the content, an etiologic agent may not be found or may be _ due to imoporer specimen collections.

<p>destroyed</p> Signup and view all the answers

Match each term with their definition:

<p>Pathology = The study of the structural and functional manifestation of disease. Pathologist = A physician who has had an extensive, specialized training in pathology. Anatomical Pathology = Where pathologist performed autopsies in the morgue and examine diseased organs, stained tissue sections and cytology specimen. Clinical pathology = Consist of blood bank, clinical microscopy, hematology , immunology lab</p> Signup and view all the answers

Flashcards

Physical Examination

Examination of a patient's body to detect signs of disease.

Clinical Specimens

Blood, feces, sputum, wound.

Importance of High Quality Specimen

Achieving accurate, clinically relevant laboratory results.

Specimen Quality Components

Proper selection, collection, and transport.

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Effects of Improper Specimen Collection

Etiologic agent may be missed; overgrowth by microflora may mask the pathogen; contaminants may interfere.

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Specimen Collection Site

Collect from site where pathogen is most likely to be found.

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Specimen Before Antimicrobials

Collect a specimen before initiating antimicrobial therapy.

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Best Time for Specimen Collection

Collect during the acute phase of the disease.

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Sterile Specimen Containers

Specimens placed/collected in a sterile container prevent contamination.

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Pathology

Study of structural and functional disease manifestations.

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Anatomical Pathologist

Performs autopsies and examines diseased tissues.

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Clinical Pathology

Consists of blood bank, clinical microscopy, hematology, and immunology lab.

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Study Notes

  • Proper diagnosis of infection is vital in microbiology and parasitology.
  • It requires patient history, physical examination, evaluation of signs and symptoms, and appropriate clinical specimen collection.

Clinical Specimens

  • Blood, feces, sputum, and wound specimens may be taken
  • Urine, cerebrospinal fluids and throat swabs may be taken

High Quality Specimens

  • High quality specimens are critical for accurate, clinically relevant laboratory results.
  • They provide information to properly diagnose a patient's infectious disease.

Specimen Quality Components

  • Proper specimen selection, collection, and transport to the laboratory are crucial.

Effects of Improper Collection

  • The etiologic agent may be missed or destroyed if not taken properly.
  • Indigenous microflora overgrowth can mask the pathogen.
  • Contaminants can interfere with results.

Specimen Selection, Collection, and Transport:

  • Specimens must be properly and carefully selected and collected.
  • Collect material from the most likely site of the suspected pathogen.
  • Obtain specimens ideally before starting antimicrobial therapy.
  • The acute stage of the disease is the best for specimen collection.
  • Collect specimens with care to avoid harming the patient.
  • Get a sufficient specimen quantity for all required diagnostic tests.
  • Use sterile containers to prevent contamination.
  • Protect specimens from heat and cold, deliver them to the lab promptly.
  • Handle hazardous specimens with extra care to avoid contamination.
  • Use sterile/disposable specimen containers as much as possible.
  • Specimen containers should be labeled properly.

Terminologies:

  • Pathology is the study of the structural and functional manifestations of disease.
  • A pathologist is a physician with extensive, specialized pathology training.
  • Anatomical pathology involves pathologists performing autopsies and examining diseased organs, tissue sections, and cytology specimens.
  • Clinical pathology includes blood bank, clinical microscopy, hematology, and immunology lab work.

Specimens for Bacteria

  • Blood, CSF, conjunctival swab, nasal swab, and synovial fluid may be taken for specimens
  • Bone marrow, cervical & vaginal swab, feces & rectal swab, pus from wound, throat swab may be taken for specimens
  • Sputum, urogenital secretion, and tissue specimen may be taken for specimens

Specimens for Virus

  • Blood, conjunctival swab, and feces & rectal swab may be taken for specimens
  • Bronchial washes, tissue specimen, and throat swab may be taken for specimens
  • CSF and urine may be taken for specimens

Specimens for Fungi

  • Blood and sputum may be taken for specimens
  • Hair clipping and nail clipping may be taken for specimens
  • CSF and tissue specimen may be taken for specimens
  • Skin scraping may be taken for specimens

Specimens for Parasite

  • Blood, scotch tape prep, skin snip, and tissue specimen may be taken for specimens
  • CSF and urogenital secretion may be taken for specimens
  • Sputum and urine may be taken for specimens
  • Feces/rectal swab may be taken for specimens

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