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Denaturation of Proteins and Nucleic Acids

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Questions and Answers

What is the importance of denaturation in DNA replication or transcription process?

It partially 'opens' the DNA, allowing replication or transcription to proceed (correct)
It helps in the polymerization of amino acids
It enhances the expression of neurotransmitters in the brain
It increases the synthesis of non-essential amino acids

What is the role of denaturation in the human body's defense against pathogens?

It aids in the digestion of proteins
It promotes the expression of essential amino acids
It helps in the synthesis of neurotransmitters
It is used to kill pathogens through pH regulation and biochemical secretions (correct)

What is the significance of denaturation in food digestion?

It plays a role in the expression of essential amino acids
It helps in the synthesis of neurotransmitters
It increases the synthesis of non-essential amino acids
It aids in the denaturation of proteins by digestive enzymes (correct)

What is the primary function of the anticodon arm in tRNA?

Base pairing with the codon of the mRNA (C) Signup and view all the answers

What is the application of denaturation in a polymerase chain reaction?

It is used to produce several in vitro copies of DNA quickly (A) Signup and view all the answers

What is the role of denaturation in the medical field?

It is used to kill bacteria, viruses, and other diseased cells (A) Signup and view all the answers

What is the result of protein hydrolysis?

Breakdown of proteins into free amino acids (B) Signup and view all the answers

What is the role of the ribosome in cells?

Protein synthesis (D) Signup and view all the answers

What is the significance of denaturation in laboratory research?

It is a favored process in a polymerase chain reaction (D) Signup and view all the answers

What is the consequence of protein denaturation?

Loss of biological function (B) Signup and view all the answers

What is the term for the tRNA that holds the growing polypeptide chain?

Peptidyl-tRNA (C) Signup and view all the answers

What is the purpose of amino acid analysis in protein hydrolysis?

To determine the amino acid composition and concentration of proteins (B) Signup and view all the answers

What is the result of a protein being exposed to a denaturant?

Its structure is altered resulting in the loss of its innate biological activity and function (B) Signup and view all the answers

Which of the following can cause denaturation of DNA?

Exposure to heat (B) Signup and view all the answers

What is the effect of violent whipping or shaking on protein structure?

It disrupts hydrogen bonds and causes the protein to denature (D) Signup and view all the answers

What is the purpose of denaturation in cellular processes?

To regulate DNA processes (D) Signup and view all the answers

What is the effect of reducing agents on protein structure?

They reduce disulfide linkages to produce –SH groups (C) Signup and view all the answers

What is the mode of action of salts of heavy metals as denaturants?

They disrupt hydrogen bonds and salt bridges (D) Signup and view all the answers

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