Denaturation of Proteins and Nucleic Acids
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Questions and Answers

What is the importance of denaturation in DNA replication or transcription process?

  • It partially 'opens' the DNA, allowing replication or transcription to proceed (correct)
  • It helps in the polymerization of amino acids
  • It enhances the expression of neurotransmitters in the brain
  • It increases the synthesis of non-essential amino acids
  • What is the role of denaturation in the human body's defense against pathogens?

  • It aids in the digestion of proteins
  • It promotes the expression of essential amino acids
  • It helps in the synthesis of neurotransmitters
  • It is used to kill pathogens through pH regulation and biochemical secretions (correct)
  • What is the significance of denaturation in food digestion?

  • It plays a role in the expression of essential amino acids
  • It helps in the synthesis of neurotransmitters
  • It increases the synthesis of non-essential amino acids
  • It aids in the denaturation of proteins by digestive enzymes (correct)
  • What is the primary function of the anticodon arm in tRNA?

    <p>Base pairing with the codon of the mRNA</p> Signup and view all the answers

    What is the application of denaturation in a polymerase chain reaction?

    <p>It is used to produce several in vitro copies of DNA quickly</p> Signup and view all the answers

    What is the role of denaturation in the medical field?

    <p>It is used to kill bacteria, viruses, and other diseased cells</p> Signup and view all the answers

    What is the result of protein hydrolysis?

    <p>Breakdown of proteins into free amino acids</p> Signup and view all the answers

    What is the role of the ribosome in cells?

    <p>Protein synthesis</p> Signup and view all the answers

    What is the significance of denaturation in laboratory research?

    <p>It is a favored process in a polymerase chain reaction</p> Signup and view all the answers

    What is the consequence of protein denaturation?

    <p>Loss of biological function</p> Signup and view all the answers

    What is the term for the tRNA that holds the growing polypeptide chain?

    <p>Peptidyl-tRNA</p> Signup and view all the answers

    What is the purpose of amino acid analysis in protein hydrolysis?

    <p>To determine the amino acid composition and concentration of proteins</p> Signup and view all the answers

    What is the result of a protein being exposed to a denaturant?

    <p>Its structure is altered resulting in the loss of its innate biological activity and function</p> Signup and view all the answers

    Which of the following can cause denaturation of DNA?

    <p>Exposure to heat</p> Signup and view all the answers

    What is the effect of violent whipping or shaking on protein structure?

    <p>It disrupts hydrogen bonds and causes the protein to denature</p> Signup and view all the answers

    What is the purpose of denaturation in cellular processes?

    <p>To regulate DNA processes</p> Signup and view all the answers

    What is the effect of reducing agents on protein structure?

    <p>They reduce disulfide linkages to produce –SH groups</p> Signup and view all the answers

    What is the mode of action of salts of heavy metals as denaturants?

    <p>They disrupt hydrogen bonds and salt bridges</p> Signup and view all the answers

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