Decalcification Quiz
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Questions and Answers

What is the purpose of decalcification in histotechnologist's work?

The purpose of decalcification is to enable the histotechnologist to cut soft sections of the bone using the microtome.

How does decalcification assist in the cutting process of bone sections?

Decalcification softens the bone, allowing the histotechnologist to cut thin sections using the microtome.

What tool does the histotechnologist use to cut soft sections of the bone?

The histotechnologist uses a microtome to cut soft sections of the bone after decalcification.

Study Notes

Decalcification in Histotechnology

  • Decalcification removes calcium salts from bone tissue, facilitating the process of sectioning.
  • This allows for the preparation of thin, microscopic bone sections for analysis.
  • Decalcification converts hard bone tissue into a softer, more pliable state.

The Cutting Process

  • The removal of calcium enables the bone to be cut with a microtome, a specialized tool for cutting tissue sections.
  • Microtomes are equipped with extremely sharp blades that slice through the softened bone tissue.
  • The thin slices, known as sections, are then mounted on slides for microscopic examination.

Microtomes

  • Microtomes are essential equipment in histotechnology, specifically designed for precise tissue sectioning.
  • They come in different types, with rotary microtomes being commonly used for bone tissue processing.
  • The rotary microtome allows for the creation of uniform sections, enabling accurate visualization of bone structures under a microscope.

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Description

Test your knowledge on decalcification with this quiz! Learn about the histological process of removing calcium ions from bones and calcified tissues, making them more flexible and easier to cut.

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